Gill developmental program in the teleost mandibular arch
- Eli and Edythe Broad California Institute for Regenerative Medicine Center for Regenerative Medicine and Stem Cell Research, Department of Stem Cell Biology and Regenerative Medicine, University of Southern California Keck School of Medicine, United States
- Marine Biological Laboratory, United States
- Department of Zoology, University of Cambridge, United Kingdom
Figures
Figure 1 with 1 supplement
The zebrafish pseudobranch derives from mandibular arch mesenchyme and first pouch epithelia.
(a), Schematic showing the pseudobranch (arrows), gill filaments (branched green structures) connected to gill bars (blue), teeth (purple), vasculature (pink), and jaw and jaw-support skeleton …
Figure 1—figure supplement 1
Development of zebrafish pseudobranch and lineage analysis of gill filament epithelia.
(a) In Sox10:Cre; acta2:loxP-BFP-Stop-loxP-dsRed fish at 5 dpf, the developing pseudobranch (white arrow) and gill buds (yellow arrow) consist of Cre-converted dsRed+ neural crest-derived mesenchyme …
Figure 2
Shared regulatory program for pseudobranch and gill development.
(a-c) In the pseudobranch (white arrows) and gill filaments (yellow arrows), gata3-p1:GFP labels growing buds, ucmaa-p1:GFP labels cellular cartilage (distinct from hyaline cartilage, arrowhead), …
Figure 3 with 2 supplements
Pseudobranch and gill development requires gata3 function.
(a) Similar expression of gata3 and gata2a in developing pseudobranch (white arrows) and gill regions (yellow arrows). (b) Sox10:Cre; acta2:loxP-BFP-Stop-loxP-dsRed labels Cre-converted dsRed+ …
Figure 3—figure supplement 1
Pseudobranch shares gene expression with gill filaments.
(a) Expression of ucmaa in pseudobranch cartilage from one-year-old fish. (b,c) Developing pseudobranch (white arrows) and gill buds (yellow arrows) express gata3 and gata2a at 5 dpf. DAPI labels …
Figure 3—figure supplement 2
The pseudobranch shares irx5a-p1 pillar cell enhancer activity with gill filaments.
(a) An intergenic irx5a-p1 region displays accessible chromatin specifically in pillar cells at 60 dpf. (b) irx5a-p1 drives GFP expression in pillar cells of the developing gills (yellow arrows) and …
Author response image 1
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Danio rerio) | ucmaa | Ensembl: ENSDARG00000027799 | ||
| Gene (Danio rerio) | gata3 | Ensembl: ENSDARG00000016526 | ||
| Gene (Danio rerio) | gata2a | Ensembl: ENSDARG00000059327 | ||
| Gene (Danio rerio) | irx5a | Ensembl: ENSDARG00000034043 | ||
| Genetic reagent (Danio rerio) | Tübingen | ZIRC | RRID:ZIRC_ZL57 | Wildtype strain of zebrafish |
| Genetic reagent (Danio rerio) | Tg(fli1a:eGFP)y1 | Lawson and Weinstein, 2002 | ||
| Genetic reagent (Danio rerio) | Tg(sox10:kikGR)el2 | Balczerski et al., 2012 | ||
| Genetic reagent (Danio rerio) | Tg(ucmaa_p1:GFP, cryaa:Cerulean)el851 | Fabian et al., 2022 | ||
| Genetic reagent (Danio rerio) | Tg(gata3_p1:GFP, cryaa:Cerulean)el858 | Fabian et al., 2022 | ||
| Genetic reagent (Danio rerio) | Tg(fgf10b:nEOS)el865 | Fabian et al., 2022 | ||
| Genetic reagent (Danio rerio) | Tg(–3.5ubb:loxP-STOP-loxP-mCherry)el818 | Fabian et al., 2020 | ||
| Genetic reagent (Danio rerio) | Tg(Mmu.Sox10-Mmu.Fos:Cre)zf384 | Kague et al., 2012 | ||
| Genetic reagent (Danio rerio) | Tg(actab2:loxP-BFP-STOP-loxP-dsRed)sd27 | Kobayashi et al., 2014 | ||
| Genetic reagent (Danio rerio) | Tg(−6.5kdrl:mCherry)ci5 | Proulx et al., 2010 | ||
| Genetic reagent (Danio rerio) | Tg(–5.0sox17:Cre-ERT2,myl7:DsRed)sid1Tg | Hockman et al., 2017 | ||
| Genetic reagent (Danio rerio) | Tg(cdh1:mlanYFP)xt17Tg | Cronan and Tobin, 2019 | ||
| Genetic reagent (Danio rerio) | gata3b1075 | Sheehan-Rooney et al., 2013 | ||
| Genetic reagent (Danio rerio) | Tg(irx5a-p1:GFP, cryaa:Cerulean)el859 | This paper | See Materials and Methods, Section Zebrafish Lines | |
| Recombinant DNA reagent | PCS2FA-transposase | Tol2Kit | PUBMED: 17937395 396.pCS2-transposase | |
| Recombinant DNA reagent | pDestTol2AB2-irx5a-p1-E1B:GFP_pA | This paper | See Materials and Methods, Section Zebrafish Lines | |
| Sequence-based reagent | ucmaa RNAScope probe (Danio rerio); Channel 1 | ACD Bio | ||
| Sequence-based reagent | gata2a RNAScope probe (Danio rerio); Channel 1 | ACD Bio | ||
| Sequence-based reagent | gata3 RNAScope probe (Danio rerio); Channel 2 | ACD Bio | ||
| Commercial assay or kit | In-Fusion HD Cloning Plus | Takara | Takara:638,910 | |
| Commercial assay or kit | RNAScope Multiplex Fluorescent v2 Assay | ACD Bio | ACD Bio:323,100 | |
| Other | Draq5 nuclear dye | Abcam | Abcam:Ab108410 | See Materials and Methods, Section Imaging |
