A timer gene network is spatially regulated by the terminal system in the Drosophila embryo
- University of Cambridge, United Kingdom
Abstract
In insect embryos, anteroposterior patterning is coordinated by the sequential expression of the 'timer' genes caudal, Dichaete and odd-paired, whose expression dynamics correlate with the mode of segmentation. In Drosophila, the timer genes are expressed broadly across much of the blastoderm, which segments simultaneously, but their expression is delayed in a small 'tail' region, just anterior to the hindgut, which segments during germband extension. Specification of the tail and the hindgut depends on the terminal gap gene tailless, but beyond this the regulation of the timer genes is poorly understood. We used a combination of multiplexed imaging, mutant analysis, and gene network modelling to resolve the regulation of the timer genes, identifying 11 new regulatory interactions and clarifying the mechanism of posterior terminal patterning. We propose that a dynamic Tailless expression gradient modulates the intrinsic dynamics of a timer gene cross-regulatorymodule, delineating the tail region and delaying its developmental maturation.
Data availability
All necessary data are included in the main text, appendices, and supplementary information. The confocal imaging dataset on which this study is based is freely available to download from the BioImage Archive (http://www.ebi.ac.uk/bioimage-archive) under accession number S-BIAD582. This 335 GB dataset contains multiplexed image stacks of more than 800 individual embryos, including 12 different genotypes and over 50 different genotype / gene product combinations. Image analysis code is provided Appendix 2-Figure 1-source data 1. A list of the corresponding image file(s) within the dataset for all figure panels within the main text, appendices, and supplementary information is provided in Appendix 2-Figure 1-source data 2. Source Data files are provided for the expression traces in the main and supplementary figures.
Article and author information
Author details
Matthew Alan Benton
Funding
Biotechnology and Biological Sciences Research Council (Research Grant BB/P009336/1)
- Erik Clark
Trinity College, University of Cambridge (Junior Research Fellowship)
- Erik Clark
European Molecular Biology Organization (Postodoctoral Fellowship ALTF 383-2018)
- Erik Clark
Deutsche Forschungsgemeinschaft (Research Fellowship BE 6732/1-1)
- Matthew Alan Benton
Isaac Newton Trust (Research Grant)
- Matthew Alan Benton
Department of Zoology, University of Cambridge
- Matthew Alan Benton
Wellcome Trust (PhD Studentship)
- Margherita Battistara
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2022, Clark et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 2,905
- views
-
- 298
- downloads
-
- 13
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 13
- citations for umbrella DOI https://doi.org/10.7554/eLife.78902
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
A timer gene network is spatially regulated by the terminal system in the Drosophila embryo
eLife 11:e78902.
https://doi.org/10.7554/eLife.78902
