Insulin sensitivity is preserved in mice made obese by feeding a high starch diet
Figures

High starch (Hi-ST) and high fat (Hi-F) similarly increase body weight, fat mass, and food intake without changes in lean mass or energy expenditure.
(A) Body weight, n=18–20, (B) lean mass, (C) fat mass, n=8–10, (D) food intake, n=4 (averaged within cages), (E) energy expenditure, (F) 24 hr average of energy expenditure, (G) relationship between energy expenditure and body weight, (H) ambulatory activity, (I) respiratory exchange ratio (RER), (J) 24 hr average of RER, n=8–10. Grey – chow, blue – Hi-ST, pink – Hi-F. Data presented as mean ± SEM. Data analysed with a one-way ANOVA or two-way repeated measures ANOVA where appropriate. Panel G analysed by linear regression. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 1—source data 1
Raw data for Figure 1.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig1-data1-v2.xlsx

Glucose tolerance of high starch (Hi-ST) mice is similar to chow despite increased adiposity and may be due to liver, but not β-cell, involvement.
(A) Oral glucose tolerance test (oGTT) after 4 weeks of feeding, (B) area under the curve for the oGTT after 4 weeks of feeding, n=45, (C) insulin released during the oGTT after 4 weeks of feeding, n=42–45. (D) oGTT after 12 weeks of feeding, (E) area under the curve for the oGTT after 12 weeks of feeding, (F) insulin released during the oGTT after 12 weeks of feeding, n=19–20. (G) oGTT with deuterated glucose after 14 weeks of feeding, n=10, (H) deuterated glucose enrichment curves, (I) endogenous glucose production during the oGTT, (J) endogenous glucose production as expressed as a change from baseline, n=10. (K) Glucose-stimulated insulin secretion, n=5–6, (L) insulin content of β-cell (53–60 islets). Grey – chow, blue – Hi-ST, pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a one-way ANOVA or two-way repeated measures ANOVA where appropriate. *p<0.05 different to chow; #p<0.05 different to Hi-ST; ‡p<0.05 different to 2.8 of the same dietary group.
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Figure 2—source data 1
Raw data for Figure 2.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig2-data1-v2.xlsx

Euglycaemic-hyperinsulinaemic clamp parameters show preservation of whole body, liver and skeletal muscle insulin resistance.
(A) Blood glucose, (B) plasma insulin, (C) glucose infusion rate, (D) average glucose infusion rate of 90–120 min, (E) whole body glucose disappearance, (F) hepatic glucose output (HGO), (G) percent suppression of HGO, (H) muscle glucose uptake, (I) adipose tissue glucose uptake, (J) phosphorylation of Akt in liver after the clamp, (K) phosphorylation of Akt in quadriceps muscle after the clamp, (L) phosphorylation of Akt in epididymal white adipose tissue (eWAT) after the clamp, (M) phosphorylation of Akt in subcutaneous white adipose tissue (sWAT) after the clamp. B, basal state; C, clamped state. Grey – chow, blue – high starch (Hi-ST), pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a one-way ANOVA or two-way repeated measures ANOVA where appropriate. n=8–11. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 3—source data 1
Raw data for Figure 3.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig3-data1-v2.xlsx
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Figure 3—source data 2
Raw western blot images for Figure 3.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig3-data2-v2.zip

Adipose tissue weights, and adipocyte size, were increased to a similar extent in high starch (Hi-ST) and high fat (Hi-F) as were plasma leptin and the level of triglycerides in liver and muscle.
(A) Tissue weights, n=9–11, (B) average adipocyte size in epididymal white adipose tissue (WAT), (C) average adipocyte size in subcutaneous WAT, n=3–5, plasma levels of (D) leptin and (E) adiponectin, n=10–11. (F) Liver triglyceride levels, n=5–8, (G) quadriceps muscle triglyceride levels, n=9–12. Grey – chow, blue – Hi-ST, pink – Hi-F. Data presented as mean ± SEM. Data analysed with a one-way ANOVA. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 4—source data 1
Raw data for Figure 4.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig4-data1-v2.xlsx

The increased liver triglycerides in the high starch (Hi-ST) mice was due to an upregulation of the de novo lipogenic pathway.
(A) Liver triglycerides, (B) liver lipogenic rate, (C) brown adipose tissue (BAT) lipogenic rate, (D) white adipose tissue (WAT) lipogenic rates, n=12–15, (E) western blots of acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD-1), (F) densitometry of protein levels of ACC, FAS, and SCD-1, (G) ATP citrate lysase (ACL) activity and (H) glucose-6-phosphate dehydrogenase (G6PDH) activity, n=9–10. Grey – chow, blue – Hi-ST, pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a one-way ANOVA. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 5—source data 1
Raw data for Figure 5.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig5-data1-v2.xlsx
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Figure 5—source data 2
Raw western blot images for Figure 5.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig5-data2-v2.zip

Reduction in total ceramide levels, as well as specific ceramide species that are correlated with insulin sensitivity, is found in the liver and quadriceps muscle of high starch (Hi-ST) fed mice.
(A) Total lipid levels, (B) diacylglycerol (DAG) species, (C) ceramide species, and (D) the relationship between insulin sensitivity and Cer18:0 levels in muscle. (E) Total lipid levels, (F) DAG species, (G) ceramide species, and (H) the relationship between insulin sensitivity and Cer22:0 levels in liver. Grey – chow, blue – Hi-ST, pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a Kruskal-Wallis test with pairwise Wilcoxon rank sum used for post hoc comparisons. Data were corrected for false discovery rate using the method of Benjamini-Hochberg. n=8–11. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 6—source data 1
Raw data for Figure 6.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig6-data1-v2.xlsx

Levels of sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylethanolamine (PE), or phosphatidylserine (PS) in muscle.
Grey – chow, blue – high starch (Hi-ST), pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a Kruskal-Wallis test with pairwise Wilcoxon rank sum used for post hoc comparisons. Data were corrected for false discovery rate using the method of Benjamini-Hochberg. n=9–11. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 6—figure supplement 1—source data 1
Raw data for Figure 6—figure supplement 1.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig6-figsupp1-data1-v2.xlsx

Levels of sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and cholesterol esters (CE) in liver.
Grey – chow, blue – high starch (Hi-ST), pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a Kruskal-Wallis test with pairwise Wilcoxon rank sum used for post hoc comparisons. Data were corrected for false discovery rate using the method of Benjamini-Hochberg. n=9–11. *p<0.05 different to chow; #p<0.05 different to Hi-ST.
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Figure 6—figure supplement 2—source data 1
Raw data for Figure 6—figure supplement 2.
- https://cdn.elifesciences.org/articles/79250/elife-79250-fig6-figsupp2-data1-v2.xlsx
Tables
Enzyme activities in liver and muscle.
Enzyme activityμmol/min/g protein | Chow | Hi-ST | Hi-F |
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Liver | |||
Citrate synthase | 110.8±7.3 | 140.1±3.0* | 119.9±6.1 |
βHAD | 100.4±5.2 | 117.0±2.1 | 122.7±7.0* |
Succinate dehydrogenase | 14.1±0.6 | 15.1±0.4 | 15.1±1.2 |
Pyruvate dehydrogenase | 0.90±0.21 | 0.73±0.33 | 0.36±0.07 |
Quadriceps muscle | |||
Citrate synthase | 188.7±5.1 | 208.0±6.0* | 223.1±3.7* |
βHAD | 23.9±1.2 | 28.4±1.4* | 35.4±1.2*# |
Succinate dehydrogenase | 2.7±0.1 | 2.7±0.1 | 2.5±0.2 |
Pyruvate dehydrogenase | 0.46±0.08 | 0.33±0.10 | 0.15±0.04* |
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Data expressed as mean ± SEM. n=7–10. *p<0.05 compared to chow; #p<0.05 compared to Hi-ST.
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Table 1—source data 1
Raw data for Table 1.
- https://cdn.elifesciences.org/articles/79250/elife-79250-table1-data1-v2.xlsx
Additional files
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Supplementary file 1
Tables showing (a) muscle metabolomics, (b) liver metabolomics, and (c) diet composition.
- https://cdn.elifesciences.org/articles/79250/elife-79250-supp1-v2.docx
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MDAR checklist
- https://cdn.elifesciences.org/articles/79250/elife-79250-mdarchecklist1-v2.docx
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Source data 1
Raw data for Supplementary file 1a.
- https://cdn.elifesciences.org/articles/79250/elife-79250-data1-v2.xlsx
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Source data 2
Raw data for Supplementary file 1b.
- https://cdn.elifesciences.org/articles/79250/elife-79250-data2-v2.xlsx