(A) Body weight, n=18–20, (B) lean mass, (C) fat mass, n=8–10, (D) food intake, n=4 (averaged within cages), (E) energy expenditure, (F) 24 hr average of energy expenditure, (G) relationship between …
Raw data for Figure 1.
(A) Oral glucose tolerance test (oGTT) after 4 weeks of feeding, (B) area under the curve for the oGTT after 4 weeks of feeding, n=45, (C) insulin released during the oGTT after 4 weeks of feeding, …
Raw data for Figure 2.
(A) Blood glucose, (B) plasma insulin, (C) glucose infusion rate, (D) average glucose infusion rate of 90–120 min, (E) whole body glucose disappearance, (F) hepatic glucose output (HGO), (G) percent …
Raw data for Figure 3.
Raw western blot images for Figure 3.
(A) Tissue weights, n=9–11, (B) average adipocyte size in epididymal white adipose tissue (WAT), (C) average adipocyte size in subcutaneous WAT, n=3–5, plasma levels of (D) leptin and (E) …
Raw data for Figure 4.
(A) Liver triglycerides, (B) liver lipogenic rate, (C) brown adipose tissue (BAT) lipogenic rate, (D) white adipose tissue (WAT) lipogenic rates, n=12–15, (E) western blots of acetyl CoA carboxylase …
Raw data for Figure 5.
Raw western blot images for Figure 5.
(A) Total lipid levels, (B) diacylglycerol (DAG) species, (C) ceramide species, and (D) the relationship between insulin sensitivity and Cer18:0 levels in muscle. (E) Total lipid levels, (F) DAG …
Raw data for Figure 6.
Grey – chow, blue – high starch (Hi-ST), pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a Kruskal-Wallis test with pairwise Wilcoxon rank sum used for post hoc comparisons. …
Raw data for Figure 6—figure supplement 1.
Grey – chow, blue – high starch (Hi-ST), pink – high fat (Hi-F). Data presented as mean ± SEM. Data analysed with a Kruskal-Wallis test with pairwise Wilcoxon rank sum used for post hoc comparisons. …
Raw data for Figure 6—figure supplement 2.
Enzyme activityμmol/min/g protein | Chow | Hi-ST | Hi-F |
---|---|---|---|
Liver | |||
Citrate synthase | 110.8±7.3 | 140.1±3.0* | 119.9±6.1 |
βHAD | 100.4±5.2 | 117.0±2.1 | 122.7±7.0* |
Succinate dehydrogenase | 14.1±0.6 | 15.1±0.4 | 15.1±1.2 |
Pyruvate dehydrogenase | 0.90±0.21 | 0.73±0.33 | 0.36±0.07 |
Quadriceps muscle | |||
Citrate synthase | 188.7±5.1 | 208.0±6.0* | 223.1±3.7* |
βHAD | 23.9±1.2 | 28.4±1.4* | 35.4±1.2*# |
Succinate dehydrogenase | 2.7±0.1 | 2.7±0.1 | 2.5±0.2 |
Pyruvate dehydrogenase | 0.46±0.08 | 0.33±0.10 | 0.15±0.04* |
Data expressed as mean ± SEM. n=7–10. *p<0.05 compared to chow; #p<0.05 compared to Hi-ST.
Raw data for Table 1.
Tables showing (a) muscle metabolomics, (b) liver metabolomics, and (c) diet composition.
Raw data for Supplementary file 1a.
Raw data for Supplementary file 1b.