Sequential addition of neuronal stem cell temporal cohorts generates a feed-forward circuit in the Drosophila larval nerve cord
- Department of Molecular Genetics and Cell Biology, University of Chicago, United States
- Committee on Computational Neuroscience, University of Chicago, United States
- Program in Cell and Molecular Biology, University of Chicago, United States
- Committee on Neurobiology, University of Chicago, United States
- Department of Neurobiology, University of Chicago, United States
- University of Chicago Neuroscience Institute, United States
Figures
Figure 1 with 1 supplement
Twin-spot mosaic analysis with a repressible cell marker (ts-MARCM) determines the birth order and morphology of NB3-3A1L/R neurons.
(A, B) Illustrations of Drosophila neuroblasts. (A) The nerve cord is left–right symmetrical and segmented. Each circle represents one neuroblast with NB3-3 in maroon. Segment A1 (boxed) is enlarged …
Figure 1—figure supplement 1
NB3-3-GAL4 line.
(A) Illustration of NB3-3-GAL4 line. 28H10 (blue outlined box) and EMS (orange outlined box) are promoters that drive in different subsets of neuroblasts, whose expression overlaps specifically in …
Figure 2
Twin-spot mosaic analysis with a repressible cell marker (ts-MARCM) provides birth order for all neurons in the NB3-3A1L/R lineage.
(A) Schematic of NB3-3A1 lineage progression is shown with EL birth order. Each circle represents one cell, and each arrow represents a cell division. (B, C) Images of individually labeled ts-MARCM …
Figure 3
The morphology of all NB3-3A1L/R neurons in the connectome.
(A–C) Images of NB3-3A1L neurons with clustered soma and bundled neuritis. (A, B) NB3-3-GAL4 or EL-GAL4 driving expression of membrane GFP was immortalized using a permanent labeling strategy (Supple…
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Figure 3—source data 1
NB3-3A1L/R cortex neurite length.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig3-data1-v2.xlsx
Figure 4
There are sharp transitions in lineage progression with respect to sensory neuron and interneuron inputs onto NB3-3A1L/R.
(A–D) Quantification of synaptic inputs onto NB3-3A1L/R. (A) The pie chart displays the percentage of total synapses from a given neuron type onto NB3-3A1L neurons. Data are color-coded, and the …
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Figure 4—source data 1
All synaptic input neuron information onto NB3-3A1L/R.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig4-data1-v2.xlsx
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Figure 4—source data 2
Sensory input onto NB3-3A1L/R neurons.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig4-data2-v2.xlsx
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Figure 4—source data 3
Summary of NB3-3A1L/R input neurons based on number of input.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig4-data3-v2.xlsx
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Figure 4—source data 4
NB3-3A1L/R input from L/R paired neurons.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig4-data4-v2.xlsx
Figure 5
Early-born and late-born ELs have significantly similar input connectivity patterns between different temporal cohorts.
(A–E) Quantifications of input connectivity between NB3-3A1L/R neurons using network analysis. (A) The plot shows pairwise comparisons of input connectivity with significant similarities in black …
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Figure 5—source data 1
z-scores for similarity.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig5-data1-v2.xlsx
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Figure 5—source data 2
z-scores for differences.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig5-data2-v2.xlsx
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Figure 5—source data 3
Similarity z-scores for sensory only input.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig5-data3-v2.xlsx
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Figure 5—source data 4
Similarity z-scores for interneuron only input.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig5-data4-v2.xlsx
Figure 6
At the population level and single-neuron level, four morphological classes of neurons provide the majority of synaptic input onto early-born ELs in A1.
(A–D) Images of neurons from each class. Examples of neuron morphology from connectome data. Black mesh shows the outline of the central nervous system (CNS), anterior is up, images show in dorsal …
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Figure 6—source data 1
Basin, EL, Ladder, and CHO input onto early-born ELs in A1L/R.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig6-data1-v2.xlsx
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Figure 6—source data 2
Distribution of input onto early-born ELs in A1L/R.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig6-data2-v2.xlsx
Figure 7
Early-born ELs are embedded in a feed-forward motif and encode the onset of vibrational stimuli.
(A–D) Illustrations of patterns of synaptic inputs onto early-born ELs. (A) A simplified schematic show the feed-forward motif in which early-born ELs are embedded. (B–D) Schematics of patterns of …
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Figure 7—source data 1
Breakdown of Basin, EL, Ladder, and CHO input onto individual early-born ELs.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig7-data1-v2.xlsx
Figure 8
Birth time and birth order of Basin interneurons using twin-spot mosaic analysis with a repressible cell marker (ts-MARCM).
(A) Illustration of NB3-5 lineage progression. Each circle represents one cell, and each arrow represents a cell division. The X-axis represents developmental time, and dashes represent approximate …
Figure 9
Basins are a middle-to-late-born temporal cohort in the NB3-5 lineage.
(A–E) Images of Basin interneurons and other lineage-related neurons. (A) The larval central nervous system (CNS) with Basins neurons expressing membrane GFP is shown in dorsal view with anterior …
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Figure 9—source data 1
NB3-5 input neuron information.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig9-data1-v2.xlsx
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Figure 9—source data 2
Similarity z-score for NB3-5 A1R.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig9-data2-v2.xlsx
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Figure 9—source data 3
Similarity z-score for NB3-5 A1L.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig9-data3-v2.xlsx
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Figure 9—source data 4
NB3-5 cortex neurite length.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig9-data4-v2.xlsx
Figure 10
Ladders are a temporal cohort from midline neuroblast (MNB).
(A) Illustration of MNB lineage progression. Each circle represents one cell, and arrows represent cell division. The X-axis represents developmental time, and dashes represent approximate positions …
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Figure 10—source data 1
Ladder input neuron information.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig10-data1-v2.xlsx
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Figure 10—source data 2
Similarity z-scores for Ladder neurons.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig10-data2-v2.xlsx
Figure 11
A08 interneurons that synapse onto early-born ELs in A1 are early-born ELs from segments other than A1.
(A–F) Images of early-born EL interneuron multi-color flip out (MCFO) clones along with matching A08 interneurons in the connectome. For each figure panel, the main image (e.g., A) shows neuronal …
Figure 12
Basin and Ladder interneurons are born after early-born EL interneurons.
(A) Illustration of neurons labeled by various heat shock protocols. At top, NB3-3 lineage progression is shown. Each circle represents one cell, and each arrow represents a cell division. …
Figure 13
Other interneurons that are highly connected onto early-born ELs in A1 come from multiple lineages, and a majority are born after early-born ELs.
(A) Quantification of neurons highly connected to early-born ELs. The pie chart displays highly connected inputs onto early-born ELs as a proportion of total number (70) highly connected neurons. …
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Figure 13—source data 1
Summary of amount and timing of important input onto early-born ELs.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig13-data1-v2.xlsx
Figure 14
Interneurons from the NB7-1 Notch OFF hemilineage synapse onto early-born ELs and are born after early-born ELs.
(A–C) Images of neuron A03 upstream of A08m, an interneuron from the Notch OFF NB7-1 hemilineage. (A) A confocal image shows a labeled neuron A03 upstream of A08m. This clone was generated using …
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Figure 14—source data 1
NB7-1 naming and cortex neurite length.
- https://cdn.elifesciences.org/articles/79276/elife-79276-fig14-data1-v2.xlsx
Additional files
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Supplementary file 1
Inputs onto NB3-3A1L/R neurons.
With following tabs: All inputs on NB3-3A1L/R neurons (Figure 4A). Left–right paired interneuron inputs (Figure 4D). NB3-3 to NB3-3 connectivity (Figure 11G).
- https://cdn.elifesciences.org/articles/79276/elife-79276-supp1-v2.xlsx
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Supplementary file 2
Inputs onto early-born ELs.
With following tabs: All sensory neuron and interneuron inputs onto early-born ELs (Figure 6E). Summary (Figure 6EB). Inputs onto early-born ELs (Figure 6B). Input onto each early-born EL (Figure 7B–D).
- https://cdn.elifesciences.org/articles/79276/elife-79276-supp2-v2.xlsx
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Supplementary file 3
Inputs onto Basins and Ladders.
With following tabs: Basin A1 connectivity (Figures 9F and G and 11G) Basin names (Figure 9). Ladder A1 connectivity (Figures 10F and 11G).
- https://cdn.elifesciences.org/articles/79276/elife-79276-supp3-v2.xlsx
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Supplementary file 4
Resources.
With the following tabs: Genotypes used in this study. Fly lines antibody list.
- https://cdn.elifesciences.org/articles/79276/elife-79276-supp4-v2.xlsx
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Supplementary file 5
Names for ELs in A1.
- https://cdn.elifesciences.org/articles/79276/elife-79276-supp5-v2.xlsx
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Supplementary file 6
Summary of other highly connected interneurons.
- https://cdn.elifesciences.org/articles/79276/elife-79276-supp6-v2.xlsx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/79276/elife-79276-transrepform1-v2.pdf
