(A–F) Serum levels of T3 and thyroxine (T4), 24 hr profiles of locomotor activity, body temperature, O2 consumption, and respiratory quotient are shown. Rhythm evaluation was performed by JTK_CYCLE …
(A–D) Assessment of body temperature, food and water intake (per cage, n = 8), and body weight. (E–I) Metabolic parameters (described in the y-axis) were obtained from the third week of experiment …
(A) Global (all Zeitgeber times [ZTs] included) evaluation of liver transcriptomes revealed 2336 DEGs of which 1391 and 945 were considered as up- or downregulated, respectively, using a false …
(A) Rhythmic probes were identified using the JTK_CYCLE algorithm (Supplementary file 5). Venn diagram represents the distribution of rhythmic probes for each group. (B) Rose plot of all rhythmic …
Each timepoint was averaged into a single replicate, and PCA were performed using the factoextra package in R and Hartigan-Wong, Lloyd, and Forgy MacQueen algorithms.
CircaCompare was used to evaluate the difference in rhythmic parameters, and one-sample t-test against zero value was performed (mean 0.9069 hr, p=0.0221). Sine curve was fitted for rhythmic genes …
(A, B) Genes involved in TH regulation, including transporters, Dio1, TH receptors, and well-known T3 outputs are presented. Presence (R) or absence of circadian rhythm (NR) detected by CircaCompare …
(A) Venn diagram demonstrates the number of probes that displayed differences in each rhythmic parameter (mesor, amplitude, and phase). (B) Top 5 enriched biological processes for each rhythmic …
Diurnal profile of genes from glucose (A), fatty acid (B), and cholesterol metabolism (C). Diurnal overall gene expression was normalized by CON mesor and plotted. Sine curve was fitted for rhythmic …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Mus musculus) | C57BL/6J | Janvier Labs, Germany | C57BL6JRj | |
Strain, strain background (C57BL6JRj, male) | C57BL/6JRj | Janvier Labs, Germany | 2–3-month-old male | |
Biological sample (M. musculus) | Liver and serum | Collected and immediately frozen in dry ice | ||
Sequence-based reagent | RNA extraction | TRIzol, Thermo Fisher Scientific | ||
Sequence-based reagent | RNA isolation | RNA Miniprep kit Zymo Research | ||
Sequence-based reagent | cDNA synthesis | High-Capacity Complementary DNA Reverse Transcription Kit, Thermo Fisher | ||
Sequence-based reagent | qPCR | GoTaq, Promega, USA | ||
Sequence-based reagent | Microarray | WT Plus Kit, Thermo Fisher Scientific | ||
Sequence-based reagent | qPCR primers | Integrated DNA Technologies (IDT) | Sequences are provided in the supplementary information | |
Chemical compound, drug | T3 hormone | T6397, Sigma-Aldrich | ||
Chemical compound, drug | BSA | A7906-50G, Sigma-Aldrich | ||
Commercial assay or kit | T3 detection kit | DNOV053, NovaTec | ||
Commercial assay or kit | T4 detection kit | EIA-1781, DRG Diagnostics | ||
Commercial assay or kit | Triglycerides quantification kit | MAK266, Sigma-Aldrich | ||
Commercial assay or kit | Cholesterol quantification kit | STA 384, Cell Biolabs | ||
Software, algorithm | RStudio | R 4.0.3 | ||
Software, algorithm | Prisma 9 | GraphPad |
Metabolic output rhythmic evaluation by JTK_CYCLE.
Metabolic output correlation.
Global differentially expressed genes (DEGs).
Differentially expressed genes (DEGs) per Zeitgeber time (ZT).
Rhythmic evaluation by JTK_CYCLE.
Rhythmic analyses by CircaCompare.