(A) Neurons were preincubated with vehicle, PHE and prazosin (PRAZ) before seal formation. (B) Ten consecutive traces from representative cell-attached single-channel recordings of LTCCs from … see more
(A) Neurons were preincubated with vehicle, PHE, and the indicated kinase inhibitors before seal formation. (B) Ten consecutive traces from representative cell-attached single-channel recordings of … see more
(A) Ten consecutive traces from representative cell-attached single-channel recordings of LTCCs from cultured hippocampal neurons with vehicle (H2O; black), 100 µM dihydroxyphenylglycine (DHPG, … see more
(A) Neurons were preincubated with vehicle, the phorbol ester phorbol-12-myristate-13-acetate (PMA), and the indicated kinase inhibitors before seal formation. (B) Ten consecutive traces from … see more
PMA (2 μM) in the bath solution potentiated the open probability of L-type channels during single-channel recording. Isradipine (10 μM) in the pipette solution completely blocked L-type currents in … see more
(A, E) Seals were formed by the recording pipettes before application of phenylephrine (PHE) or norepinephrine (NE) and ultimately of either isradipine or nimodipine to ensure channel activity was … see more
(A) Neurons were preincubated with NE ± prazosin (PRAZ) before seal formation. (B, C) Sample diaries show time courses of Po recordings obtained after preincubation with either NE alone or NE + PRAZ … see more
(A) Seals were formed by the recording pipettes before application of BK and ultimately of BayK8644 (BayK) to ensure channel activity was mediated by LTCCs. (B) Sample diary shows time course of Po … see more
Triton X-100 extracts were cleared from non-soluble material by ultracentrifugation before immunoprecipitation (IP) with antibodies against α11.2, Pyk2 itself, or non-immune control antibodies … see more
Original files of the full raw unedited blots with bands labeled in red boxes.
PC12 cells were pretreated with vehicle (0.02% DMSO), the Pyk2 inhibitor PF-431396 (3 μM), and the Src inhibitor PP2 (10 μM) or its inactive analogue PP3 (10 μM) for 5 min before application of … see more
IgG indicates control IP with non-immune mouse IgG. Vehicle (0.02% DMSO), PF-431396 (3 μM), PP2 (10 μM), PP3 (10 μM), or SU6656 (SU, 10 μM) were applied 5 min before phorbol-12-myristate-13-acetate … see more
(A) Lysates from HEK293T/17 cells transfected with vectors encoding rat Pyk2 (rPyk2-GFP) and either the Pyk2-targeting FIV lentivirus-derived, pVETL-Sh1-GFP (pFV-Pyk2-Sh1) or control (empty) … see more
LTPLTCC was induced by four 200 Hz tetani, each 0.5 s long, in the CA3 Schaffer collateral projections to CA1 in acute hippocampal slices from 13- to 20-week-old mice. (A) LTPLTCC required … see more
Amino acid residues of fragments of intracellular loops of CaV1.2 α1-subunit used in GST pulldown studies.
Antibody dilutions used for immunoblotting.
shRNA sequences targeting rat Pyk2 and Src.
Sequencing primers for validation of knockdown constructs.