Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation

Abstract
Data availability
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Abstract

Chromosome segregation requires both the separation of sister chromatids and the sustained condensation of chromatids during anaphase. In yeast cells, cohesin is not only required for sister chromatid cohesion but also plays a major role determining the structure of individual chromatids in metaphase. Separase cleavage is thought to remove all cohesin complexes from chromosomes to initiate anaphase. It is thus not clear how the length and organisation of segregating chromatids is maintained during anaphase in the absence of cohesin. Here we show that degradation of cohesin at the anaphase onset causes aberrant chromatid segregation. Hi-C analysis on segregating chromatids demonstrates that cohesin depletion causes loss of intrachromatid organisation. Surprisingly, TEV-mediated cleavage of cohesin does not dramatically disrupt chromatid organisation in anaphase, explaining why bulk segregation is achieved. In addition, we identified a small pool of cohesin complexes bound to telophase chromosomes in wildtype cells and show that they play a role in the organisation of centromeric regions. Our data demonstrates that in yeast cells cohesin function is not over in metaphase, but extends to the anaphase period when chromatids are segregating.

Data availability

Sequencing data have been deposited in GEO under accession codesGSE183481

The following data sets were generated

(2021) Cohesin contribution to chromatid organisation is critical during chromosome segregation.
NCBI Gene Expression Omnibus, GSE183481 Token to have access is cpghcwoajdonjix.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183481

The following previously published data sets were used

1. Garcia-Luis J
2. Lazar-Stefanita L
3. Gutierrez-Escribano P
4. Thierry A
5. Cournac A
6. García A
7. González S
8. Sánchez M
9. Jarmuz A
10. Montoya A
11. Dore M
12. Kramer H
13. Karimi MM
14. Antequera F
15. Koszul R
16. Aragon L
(2018) FACT mediates cohesin function on chromatin
GEO database. GSE118534.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118534

Article and author information

Author details

Jonay Garcia-Luis

DNA motors Group, MRC London Institute of Medical Sciences, London, United Kingdom

Competing interests
The authors declare that no competing interests exist.
Hélène Bordelet

Régulation spatiale des génomes, Institut Pasteur, Paris, France

Competing interests
The authors declare that no competing interests exist.
Agnès Thierry

Régulation spatiale des génomes, Institut Pasteur, Paris, France

Competing interests
The authors declare that no competing interests exist.
Romain Koszul

Régulation spatiale des génomes, Institut Pasteur, Paris, France

For correspondence
romain.koszul@pasteur.fr

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-3086-1173
Luis Aragon

DNA motors Group, MRC London Institute of Medical Sciences, London, United Kingdom

For correspondence
luis.aragon@ic.ac.uk

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-0634-6742

Funding

Medical Research Council (UKRI MC-A652-5PY00)

Luis Aragon

Agence Nationale de la Recherche

Romain Koszul

Wellcome Trust (100955/Z/13/Z)

Jonay Garcia-Luis

European Research Council

Romain Koszul

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.