Individual behavioral trajectories shape whole-brain connectivity in mice
- German Center for Neurodegenerative Diseases (DZNE) Dresden, Germany
- Center for Regenerative Therapies Dresden (CRTD), TU Dresden, Germany
- Division of Psychological and Social Medicine and Developmental Neurosciences, Faculty of Medicine, Germany
- Department of Neuroimaging, Institute of Psychiatry, Psychology and Neuroscience King's College, United Kingdom
- Department of Child and Adolescent Psychiatry, Faculty of Medicine, Eating Disorder Treatment and Research Center, Germany
- Department of Basic and Clinical Neuroscience, Institute of Psychiatry, Psychology and Neuroscience, King's College, United Kingdom
- MRC Centre for Neurodevelopmental Disorders, King's College, United Kingdom
Figures
Figure 1
Emergence of inter-individual differences after environmental enrichment.
Pictures (A) of the “individuality” cage—a custom build RFID cage with 70 interconnected small cages and 115 antennae distributed in the connecting tunnels. Interindividual variance (B) of behavior …
Figure 2 with 4 supplements
Neuroanatomical differences associated with exposure to an enriched environment and with variation in roaming entropy as measured by ex vivo structural MRI.
(A) Map of regional differences in absolute volume (mm3) extracted using the DSURQE mouse brain atlas comparing ENR mice (N=38) to those in standard (STD) housing (N=10). Only atlas regions of …
Figure 2—figure supplement 1
Total brain volumes (as measured by ex vivo structural magnetic resonance imaging [MRI]) do not differ significantly (2-tailed students t-test) when comparing either (a) STD (N=10)- vs. ENR-housed mice (N=38) or (b) Down (N=15) vs. Flat (N=15) subgroups of ENR-housed mice.
Each data point indicates the whole-brain volume (in mm3) for an individual mouse in each group. Solid horizontal line indicates the group mean.
Figure 2—figure supplement 2
Neuroantomical differences comapring enriched and standard housed mice as measured by ex vivo structural MR.
(a) Map of regional differences in relative volume (% total brain volume) extracted using the DSURQE mouse brain atlas comparing ENR mice (N=38) to those in standard (STD) housing (N=10). Only atlas …
Figure 2—figure supplement 3
Voxel-wise tensor-based morphometry (TBM) to map localized neuroanatomical differences between Flat and Down subgroups of ENR-housed mice.
Data shown are the percentage difference in log-scaled Jacobian determinants without correction for total brain volume (absolute volumes) between Flat (N=15) and Down (N=15) subgroups of ENR-housed …
Figure 2—figure supplement 4
Relationship between RE and local volume changes in Down and Flat roaming mice.
Data shown are the correlation (Pearson’s r) between roaming entropy slope across all four time blocks and local brain absolute volume changes (log-scaled Jacobian determinant) for mice clustering …
Figure 3 with 1 supplement
Environmental enrichment distinctively alters brain structural covariance.
Rows and columns of each matrix denote atlas-defined structures, and color intensity the correlation strength (Pearson’s correlation, red = positive, blue = negative). (A) Mice were kept either in …
Figure 3—figure supplement 1
The violin plots with overlaid data points show increased overall coefficient variance in the volume of mouse brain regions in Flat compared to Down subgroups of ENR-housed mice.
Individual data points represent the coefficient of variation (CV) for each of 182 regions of interest (ROIs) in the DSURQE mouse brain atlas. ***p < 0.001 Mann–Whitney U-test comparing the average …
Figure 4
Network based statistics confirms differential structural connectivity patterns.
Visualization of subnetworks in the contrasts (A) ENR > STD and (B) FLAT > DOWN both at a threshold of t = 2.4. In the left panels nodes are projected onto a standard mouse brain template (Allen …
Author response image 1
Neuroanatomical differences in absolute volumes between STD-ENR comparing absolute volumes for both atlas based segmentation (a) and voxel-wise tensor based morphometry (b), to the same analyses using relative volumes where inter-individual variance in mouse total brain volume is accounted for (c, d).
In panels (a) and (c), the highlighted regions shown pass statistical significance at a threshold of 5% FDR for multiple comparisons, with the colour bar indicating the effect size as standardized …
Additional files
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Supplementary file 1
Absolute volumes.
- https://cdn.elifesciences.org/articles/80379/elife-80379-supp1-v2.xlsx
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Supplementary file 2
Relative volumes.
- https://cdn.elifesciences.org/articles/80379/elife-80379-supp2-v2.xlsx
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MDAR checklist
- https://cdn.elifesciences.org/articles/80379/elife-80379-mdarchecklist1-v2.docx
