Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator
- Department of Neurosciences and Developmental Biology, University of Vienna, Austria
- Vienna Doctoral School of Ecology and Evolution (VDSEE), University of Vienna, Austria
- Friedrich Miescher Laboratory of the Max Planck Society, Germany
- University of Konstanz, Germany
Figures
Figure 1 with 2 supplements
Bilateral body symmetry of the non-bilaterian sea anemone Nematostella is BMP signaling-dependent.
(A) Bilateral body symmetry is observed in Bilateria and in anthozoan Cnidaria. (B) BMP signaling is initiated by BMP ligands binding to BMP receptors that trigger phosphorylation, assembly, and nuclear translocation of a pSMAD1/5/SMAD4 complex to regulate gene expression. (C) A BMP signaling-dependent symmetry break at late gastrula (LG) stage results in the formation of the secondary (directive) body axis in the sea anemone Nematostella. (D) BMP signaling dynamics during Nematostella development. No pSMAD1/5 is detectable in the blastula (Di). Nuclear pSMAD1/5 is localized in the blastopore lip of midgastrula (Dii), forms a gradient along the directive axis in the LG (Diii) and 2d planula (Div). By day 3, the gradient progressively disperses (Dv), and the signaling activity shifts to the eight forming endodermal mesenteries (Dvi) and to the ectodermal stripes vis-à-vis the mesenteries (arrowheads). Images (Dii–Dvi) show oral views (asterisks). Scale bars 50 µm. (E) Comparison of the direct BMP signaling targets at LG and 4dP shows little overlap. Schemes show oral views of a LG and a 4dP with red spots indicating the position of pSMAD1/5-positive nuclei in the ectoderm (light gray) and endoderm (dark gray). (F) Transcription factors, signaling molecules, and neuronal genes are overrepresented among the pSMAD1/5 targets compared to the functional distribution of 100 random genes. LG, late gastrula targets; 4dP, 4d planula targets; DE, pSMAD1/5 ChIP targets differentially expressed in BMP2/4 and/or GDF5-like morphants (padj≤0.05); 2xDE targets, pSMAD1/5 ChIP targets differentially expressed in BMP2/4 and/or GDF5-like morphants (padj≤0.05) showing ≥2-fold change in expression. (G) Fractions of each functional category of the differentially expressed pSMAD1/5 target genes (see panel F) containing BMP response elements (BREs).
Figure 1—figure supplement 1
Sequencing coverage profile shows the enrichment of pSMAD1/5 binding at the target genes.
Overlays of the three biological replicates are shown. Statistically significant peaks are marked with asterisks. Pink bars, BMP response elements (BREs).
Figure 1—figure supplement 2
Transcriptomic comparison of BMP2/4, GDF5-like, and control morphants and differential expression of pSMAD1/5 ChIP targets upon different knockdowns.
(A) In the cluster dendrogram and (B) principal component analysis, replicates of GDF5lMO (red) and StdMO (blue) transcriptomes group together, while the transcriptome of BMP2/4MO (magenta) is separated. (C, D) Volcano plots highlight differentially expressed pSMAD1/5 ChIP targets (red) in (C) BMP2/4 morphants and (D) GDF5-like morphants (padj≤0.05).
Figure 2
Expression patterns of a selection of the direct targets of BMP signaling in 2d and 4d planulae.
In lateral views, the oral end is marked with an asterisk, inlets show oral views. Scale bar 100 µm.
Figure 3
Nematostella and Bilateria share BMP signaling targets, which predominantly encode transcription factors and signaling molecules.
(A) Overlap of BMP signaling targets at comparable embryonic stages in the three-way comparison of Nematostella, Drosophila (Deignan et al., 2016), and Xenopus (Stevens et al., 2017). Orthology links were deduced by NCBI BLASTP of the respective proteomes with a cut-off e-value ≤ 1e-5, and reciprocal best BLAST hits were determined using the bit score. (B) Transcription factors and signaling molecules represent more than 60% of pSMAD1/5 targets shared between Nematostella (Nve), fly (Dme), and frog (Xla). (C) Gene names of orthologous targets shared between Nematostella, fly, and frog. For targets shared with Nematostella, genes with known expression patterns in the embryo are underlined, while genes expressed asymmetrically along the directive axis are underlined and bold.
Figure 4
zswim4-6 is a target of BMP signaling with bilaterally symmetric expression and encodes a nuclear protein.
(A) Maximum likelihood phylogeny shows that Nematostella ZSWIM4-6 clusters with ZSWIM4, ZSWIM5, and ZSWIM6 from zebrafish, frog, and mouse. (B–F’) Nematostella zswim4-6 expression follows the dynamic BMP signaling domain (see Figure 1D for comparison). Double ISH shows zswim4-6 and chd transcripts localize to the opposite sides of the directive axis. (G–I) Mosaic expression of ZSWIM4-6-EGFP under the control of the ubiquitously active EF1α promoter in F0 transgenic animals demonstrates that ZSWIM4-6 is a nuclear protein. Full-length ZSWIM4-6-EGFP is translocated into the nuclei (G), while ZSWIM4-6ΔNLS-EGFP missing the predicted nuclear localization signal NLS remains cytoplasmic (H), similar to the EGFP control (I). Exposure time was the same in all images. (J–L) Microinjection of ZSWIM4-6-EGFP mRNA results in a weak EGFP signal detectable in the nuclei of the early blastula (J), which progressively disappears towards late gastrula (K). EGFP translated from EGFP mRNA remains readily detectable (L). To visualize the weak signal in (J–K), the exposure had to be increased in comparison to (L). Asterisks mark the oral side; scale bars 100 µm.
Figure 5 with 1 supplement
zswim4-6 reacts differently to the downregulation of BMP2/4 and GDF5-like.
(A) Expression domains and putative interactions of the BMP network components in 2d embryos (based on Genikhovich et al., 2015). (B–F’) Effect of the knockdown (KD) of the individual BMP signaling components on nuclear pSMAD1/5 and on zswim4-6 expression. zswim4-6 expression is abolished upon KD of bmp2/4 and chordin, but not in KD of gdf5-l and gremlin. Sketches in (B–F) show that the gradient is lost upon BMP2/4 and Chd KD, reduced upon GDF5-like KD and expanded upon Gremlin KD (based on Genikhovich et al., 2015). (G–L) KD of bmp2/4 using different concentrations of BMP2/4MO results in either normal, bilaterally symmetric marker expression or complete radialization but no intermediate phenotypes. Instead, the penetrance of the radialization phenotype increases with the increase of the BMP2/4MO concentration. Co-injection of the lowest, ineffective BMP2/4MO concentration with GDF5lMO also results in a complete radialization. All stainings are performed on 2d planulae. Scale bars 100 µm.
Figure 5—figure supplement 1
Two type I BMP receptors display partial redundancy in regulating zswim4-6 expression.
Expression of BMP receptors (A, A’) alk2, (B, B’) alk3/6, and (C, C’) bmprII is broadly detectable in endoderm with a slightly stronger expression on the ‘strong BMP signaling’ side of the directive axis, opposite to chordin (red). (D) qPCR quantification of knockdown (KD) efficiency for BMP receptor shRNAs in relation to shRNA against mOrange (sh_control, 100% expression). Black lines represent the median. (E–I) EGFP signal in late gastrula embryos injected with (E) EGFP mRNA and (F–I) mRNA of dominant-negative BMP receptor constructs in which Ser/Thr-kinase domain was replaced with the EGFP coding sequence. (J) Overview of the expected effect of the dominant negative BMP receptor overexpression and BMP receptor RNAi on BMP signaling. (K–O) zswim4-6 expression is weakly reduced upon overexpression of the dominant negative type I BMP receptors dnAlk2-EGFP and dn_Alk3/6-EGFP and strongly suppressed upon combined overexpression of dn_Alk2-EGFP and dn_Alk3/6-EGFP but remains unchanged upon overexpression of the dominant negative type II BMP receptor dn_BMPRII-EGFP. (P–T) Expression of zswim4-6 is weakly reduced upon Alk2 RNAi, the effect is stronger upon Alk3/6 KD, and zswim4-6 is practically abolished in a combined Alk2+Alk3/6 KD. zswim4-6 expression is unaffected by the BMPRII RNAi. Asterisks mark the oral side. Scale bars 100 µm. The numbers in the bottom-right corner indicate the ratio of embryos displaying the phenotype shown in the image to the total number of embryos treated and stained as indicated in the figure. In (P–T), KD efficiency is shown in the top-right corner of each image.
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Figure 5—figure supplement 1—source data 1
Source data for Figure 5—figure supplement 1D.
- https://cdn.elifesciences.org/articles/80803/elife-80803-fig5-figsupp1-data1-v1.txt
Figure 6 with 5 supplements
ZSWIM4-6 is a modulator of BMP signaling that appears to act as transcriptional repressor.
(A–B’’) Morpholino knockdown (KD) of zswim4-6 results in patterning defects. (A) and (B) show confocal sections across the pharyngeal region of 4d planulae stained with antiHoxE antibody (Genikhovich et al., 2015) and phalloidin. (A’) and (B’) show the areas boxed in (A) and (B). White dotted lines delineate mesenterial chambers. In the 4d planula, the HoxE-positive mesenterial chamber does not reach the pharynx, which leads to the fusion of neighboring chambers (compare A, A’ with B, B’). This results in the formation of three instead of four tentacles in the 9d polyp (compare A’’ with B’’). (C–F’) Immunofluorescence and quantification of relative nuclear anti-pSMAD1/5 staining intensities in 2d ZSWIM4-6 morphants (C–D’) and upon zswim4-6 mRNA overexpression in the late gastrula (E–F’). Intensity measurements (arbitrary units, a.u.) are plotted as a function of the relative position of each nucleus in the endoderm or in the ectoderm along a 180° arc from 0 (high signaling side) to π (low signaling side). The measurements from Control MO embryos (n = 10) and ZSWIM4-6MO embryos (n = 10), as well as egfp mRNA embryos (n = 22 for the endodermal, and n = 24 for the ectodermal measurements) and zswim4-6 mRNA embryos (n = 8 for the endodermal, and n = 9 for the ectodermal measurements) are described by a LOESS smoothed curve (solid line) with a 99% confidence interval for the mean (shade). For visualization purposes, the intensity values were normalized to the upper quantile value among all replicates and conditions of each control-experiment pair. (G–K’) Expression of zswim4-6 and BMP network components in the 2d planula upon morpholino KD of zswim4-6; All images except for (H) and (H’) show oral views. (L–P’) Expression of zswim4-6 and BMP network components in late gastrula (30 hr) upon zswim4-6 mRNA injection; oral views, purple dashed line marks the loss of a sharp boundary of chd expression. In (A–P’), asterisks mark the oral side. In (A-P’), scale bars 100 µm. (Q) ChIP with GFP-Trap detects ZSWIM4-6-EGFP fusion protein in the vicinity of the pSMAD1/5 binding site in the upstream regulatory region of chordin but not of gremlin. Experiment on biological quadruplicates. Mean enrichments and standard deviations are shown.
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Figure 6—source data 1
Source data for Figure 6D-F’.
- https://cdn.elifesciences.org/articles/80803/elife-80803-fig6-data1-v1.zip
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Figure 6—source data 2
Source data for Figure 6Q.
- https://cdn.elifesciences.org/articles/80803/elife-80803-fig6-data2-v1.txt
Figure 6—figure supplement 1
Testing ZSWIM4/6 morpholino specificity.
(A) Fluorescent signal in embryos injected with mRNA coding for the ZSWIM4/6 recognition sequence fused to the mCherry (wt-zswim4/6-mCh) coding sequence. (B) Co-injection with ZSWIM4/6 morpholino can suppress the translation of mRNAs containing the respective recognition sequence. (C) Translation of mRNA coding for the zswim4/6 recognition sequence carrying five mismatches and fused to mCherry (mismatch-zswim4/6-mCh) is no longer suppressed when co-injected with ZSWIM4/6 morpholino.
Figure 6—figure supplement 2
zzswim5 overexpression dampens BMP signaling and causes developmental defects in zebrafish.
(A–F”) Wild-type zebrafish embryos were left uninjected (B) or injected at the one-cell stage with 20, 40, 80, or 210 pg zzswim5 mRNA. (A) Phenotype quantification at 1 d post-fertilization (dpf) shows increasingly severe developmental defects at higher amounts of mRNA. A representative selection was imaged at 1 dpf (B–F’’). Multiple embryos are shown for 80 (E–E”) and 210 (F–F”) pg to illustrate the variety of defects. Number of embryos – uninjected: 19, 20 pg: 20, 40 pg: 24, 80 pg: 21, 210 pg: 17. (G–I) Embryos were injected with 80 pg zzswim5 mRNA or left uninjected and fixed at 50% epiboly (early gastrulation). BMP signaling levels were assessed using pSmad1/5/9 immunostaining. Animal pole views are shown with ventral on the left. (G) Quantification of immunofluorescence (arbitrary units, a.u.) reveals lower amplitude BMP signaling gradients in zzswim5-overexpressing embryos (I) compared to uninjected controls (H). The measurements from uninjected embryos (n = 8) and zzswim5-injected embryos (n = 8) are described by a LOESS smoothed curve (solid line) with a 99% confidence interval for the mean (shade).
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Figure 6—figure supplement 2—source data 1
Source data for Figure 6—figure supplement 2G.
- https://cdn.elifesciences.org/articles/80803/elife-80803-fig6-figsupp2-data1-v1.zip
Figure 6—figure supplement 3
ZSWIM4-6 knockdown partially rescues the reduction of pSMAD1/5 caused by GDF5-like knockdown.
Oral views of 2 dpf embryos stained for pSMAD1/5. (A, B) Embryos injected with control morpholino and ZSWIM4-6MO show phenotypes identical to the ones presented in Figure 6C and C’. (C, D) Embryos injected with GDF5lMO together with either Control MO (C) or ZSWIM4-6MO (D). pSMAD1/5 is strongly reduced upon GDF5lMO injection (C), but partially rescued by co-injection of ZSWIM4-6MO (D). (E, F) Quantification of the ectodermal (E) and the endoderm (F) nuclear pSMAD1/5 staining intensity in the GDF5lMO morphants. Nuclear staining intensity (arbitrary units, a.u.) is plotted as a function of the relative position of each nucleus in the endoderm or in the ectoderm along a 180° arc from 0 (high signaling side) to π (low signaling side). The measurements from GDF5lMO + ControlMO embryos (n = 9) and GDF5lMO + ZSWIM4-6MO embryos (n = 16) are described by a LOESS smoothed curve (solid line) with a 99% confidence interval for the mean (shade).
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Figure 6—figure supplement 3—source data 1
Source data for Figure 6—figure supplement 3E and F.
- https://cdn.elifesciences.org/articles/80803/elife-80803-fig6-figsupp3-data1-v1.zip
Figure 6—figure supplement 4
rgm expression expands upon ZSWIM4-6 knockdown.
In situ hybridization on 2d planulae. Asterisks denote the location of the blastopore. Scale bar 100 µm.
Figure 6—figure supplement 5
CRISPR/Cas9-mediated mutagenesis of zswim4-6 results in the expansion of chordin expression in mosaic mutants (F0).
(A) Bilateral expression of chordin in wild-type 2d planula and corresponding sequencing chromatogram of zswim4-6_g254 target region. (B–O) Expanded or radialized chordin expression in 2d mutant planulae injected with a single guide RNA (B–I) or two guide RNAs (J–O) targeting the SWIM zinc-finger domain. Corresponding sequencing chromatograms show sequence variability (overlapping peaks, gray or no letters) in the vicinity of the targeting locus of the zswim4-6_g254 gRNA in individual mutant embryos. The protospacer sequence is underlined, the position of the PAM is shown on (A). Scissors indicate the Cas9 cutting site. In the in situ hybridization images, asterisks mark the oral side, scale bars 100 µm.
Videos
Video 1
BMP signaling activity in late planula.
At the 4d planula larva stage, BMP signaling activity locates to the mesenteries and in the ectoderm to eight adjacent stripes that merge in pairs between the future tentacle buds to form a circumoral ring.
Additional files
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Supplementary file 1
Anti-pSMAD1/5 ChIP analysis and RNA-seq analysis of BMP2/4 and GDF5-l morphants.
Supplementary data 1 contains called peaks, curated gene list, and DE analysis of putative targets identified by anti-pSMAD1/5 ChIP. Sheet 1 (peak-calling-LG) and sheet 2 (peak-calling-4dP) contain number and positions of identified peaks at the late gastrula (LG) and in the 4 d planula (4dP) by anti-pSMAD1/5 ChIP, respectively. Sheet 3 (LG4dP-targets) depicts the curated list of putative pSMAD1/5 targets at LG and 4dP combined. Sheet 4 (BMP24MO2dvsStdMO2d-padj<0.05) and sheet 5 (GDF5lMO2dvsStdMO2d-padj<0.05) contain differentially expressed genes upon morpholino knockdown of BMP2/4 and GDF5-l with padj = 0.05. Sheet 6 (ChIP-RNA-Seq-overlap_2x) displays pSMAD1/5 targets that are differentially expressed in BMP2/4 morphants and GDF5-l morphants (2×-fold, padj = 0.05) and sheet 7 (enrichment-of-BRE-in-funct-cat) displays DE pSMAD1/5 targets containing the BRE motif.
- https://cdn.elifesciences.org/articles/80803/elife-80803-supp1-v1.xlsx
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Supplementary file 2
Trimmed alignment of ZSWIM proteins.
- https://cdn.elifesciences.org/articles/80803/elife-80803-supp2-v1.txt
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Supplementary file 3
RNA, DNA and Morpholino oligonucleotide sequences; shRNA target sites.
(A) ZSWIM4-6 guide RNA sequences and sequencing primers. (B) Primers used for cloning dominant-negative BMP receptor fragments. (C) Morpholino sequences. (D) Primer sequences for MO specificity testing. (E) Short hairpin RNA targets. (F) qPCR primers. (G) Primer sequences for in situ probes.
- https://cdn.elifesciences.org/articles/80803/elife-80803-supp3-v1.docx
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MDAR checklist
- https://cdn.elifesciences.org/articles/80803/elife-80803-mdarchecklist1-v1.pdf
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Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator
eLife 13:e80803.
https://doi.org/10.7554/eLife.80803
