(A) Transgenic mice harboring Cre recombinase cDNA downstream from a 2.5 kb fragment of the VE-Cadherin (Cdh5) mouse promoter (B6.FVB-Tg(Cdhn5-cre)7Milia) were crossed with mice with Cre-inducible …
(A) Single intra-tracheal (IT) administration of DT at doses below 20 ng was compatible with survival to 7 days post-treatment. (B) Doses above 1 ng resulted in a dose-dependent increase in right …
(A) Representative examples of Evans blue staining of lungs from binary transgenic mice. (B) Summary data showing a marked increase in Evans Blue lung content in binary transgenic mice treated with …
(A) Schematic of workflow showing the experimental design. Three separate cohorts (3, 5, and 7 days) of binary transgenic mice received DT (IT, 10 ng), and a fourth cohort of healthy animals served …
(A) All 12 barcodes were demultiplexed with a small number of cells with multiple barcodes (doublets) and a population lacking any barcode (negative). (B) Quality control was performed using the …
Expression of selected genes characterizing the identity of the major lung cell clusters based on the Tabula Muris biological atlas, including (A) Cldn5 (endothelial), (B) Ptprc (immune), (C) Epcam …
(A) Uniform manifold approximation and projection (UMAP) representation of the re-clustered endothelial populations for all cohorts produced eight distinct endothelial cell (EC) clusters. (B) The …
Typical aCap ECs genes include: (A) Car4 (Carbonic Anhydrase 4); (B) Endrb (endothelin receptor type-B); (C) Fibin (Fin Bud Initiation Factor Homolog); and (D) Tbx2 (T-Box Transcription Factor 2). …
(A) This model showed a dynamic shift of endothelial cells (ECs) through Zones 2 and 3 of transitional Cluster 1 with strong vectors towards general capillary (gCap), arterial, and venous EC …
(A) Under basal conditions (day 0; blue color) Apln was only expressed by aCap ECs (aerocytes; Cluster 2), whereas Aplnr expression was seen in gCap ECs (Cd93 positive). 3 days after EC ablation …
(A) Pdgfb (Platelet Derived Growth Factor Subunit B), (B) Npl1 (Neuropilin 1), (C) Angpt2 (Angiopoietin 2), (D) Notch1 (Notch Receptor 1), and (E) Kit (KIT Proto-Oncogene, Receptor Tyrosine Kinase). …
Different endothelial cells (ECs) populations showed very distinct profiles with the gCap proliferative ECs (Cluster 7) exhibiting 13 activated TFs, including Tfdp1 (Transcription Factor Dp-1), E2f2 …
(A) Uniform manifold approximation and projection (UMAP) dimensionality reduction reveals 13 endothelial clusters. (B) The identity of these clusters is based on markers of general capillary (gCap), …
(A) Volcano plots showing the distribution of differentially expressed genes in Clusters 1–7 (C1 to C7) according to statistical significance (Benjamini-Hochberg (BH) adjusted p-value), magnitude …
Each panel shows a predefined functional category and specific canonical signaling pathways that were significantly enriched in genes altered in each endothelial Cluster 1–7 (x-axis). Circle size …
(A) Volcano plots showing the distribution of differentially expressed genes according to statistical significance, magnitude, and direction of fold change (FC) at different points following …
Each panel shows a predefined functional category and specific canonical signaling pathways (y-axis) that were significantly enriched in genes altered at each time point (days 3, 5, and 7 versus day …
Circos plots showing color-coded sender populations (outer circle) indicating the 10 top genes signaling for each receiver population (shown in gray). (A) At day 3, only one population, general …
(A) Expression of apelin from a publicly available dataset (31) in young (3 months) versus old (24 months) mice (*=p<0.05, unpaired t-test). (B) Apelin expression assessed by qRT-PCR expressed as …
At baseline (day 0), there are two main alveolar groups of capillary ECs: larger apelin-positive aerocytes (aCap) ECs, termed aerocytes, that play a key structural role in forming the air-blood …