(A–K), Echocardiographic parameters (N=28 for TGAC8; WT = 21); (L) heart weight/body weight at sacrifice (N=75 for TGAC8 and N=85 for WT). See Supplementary file 1a for additional Echo parameters. …
(A) WB of Pathologic hypertrophy markers, (B, C) cardiac myocyte cross-sectional areas and distributions of cardiac myocyte sizes in TGAC8 and WT hearts (cardiomyocytes were counted on left free …
Raw unedited blot images and uncropped blot images of MYH7, α Sk.
Actin, ANP, BNP, and Calcineurin.
Scale bar 100 µm. N=37 high power fields of left free wall at the middle level from four WT mice. N=33 high power fields of left free wall at the middle level from four TGAC8 mice. Data are …
(A–G) representative examples of confocal images (400 x) of LV WGA (red), vimentin (Cyan), EdU (yellow), DAPI (blue), (H) average number of EdU-labeled nuclei positive field counts in LV TGAC8 vs WT …
(A) Representative examples of ADCY8 immunolabeling in TGAC8 and WT LV cardiomyocytes (scale bar 10 µm), (B) Average AC8 fluorescence in LV cardiomyocytes (N=3 animals per group; 60 cells for each …
Raw unedited blot images and uncropped blot images of PKAca, PKAr, MYH6, SERCA2, LTCC, NCX1, CamKII.
(A) Rate of global protein synthesis and (B–G) mechanisms downstream of PKA signaling involved in protein synthesis in the TGAC8 and WT. Data are presented as Mean± SEM. The statistical significance …
Raw unedited blot images and uncropped blot images of Puromycin, CITED4, CREB1, MNK1, MSK1, GAPDH, pMEK1/2, p21 Ras, p-c-Raf, total Raf, total Mek 1/2, p-ERK 1/2 and total ERK 1/2.
(A) Proteosome activity assay and (B, C) accumulated proteins in soluble and insoluble fractions of LV lysates in TGAC8 vs WT. (D) WB of HSP90 in TGAC8 and WT, (E–H) Expression levels of selected …
Raw unedited blot images and uncropped blot images of HSP90, ATG13, PARKIN, ATG4B, and PI3Kc3.
(A, B) Representative panoramic electron micrographs (white arrows depict capillaries surrounding cardiac myocytes) and (C–F) higher resolution images of LV cardiac muscle fibers and mitochondria in …
(A) LV performance and the rate of superoxide (ROS) generation in isolated working TGAC8 and WT hearts. (B) WB analysis of Nrf2 expression in LV TGAC8 vs WT. Differences between two groups were …
Raw unedited blot images and uncropped blot images of NRF2.
(A) A schematic of ATP creatine energy system, (B) Representative P31 NMR spectra of TGAC8 and WT hearts. (C–E) Average levels of ATP, PCr, and ATP/PCr in TGAC8 and WT hearts derived from NMR …
(A) Schematic of the total number of transcripts (subset ‘a’ – 11,810), and proteins (subset ‘b’ 6834), identified in LV lysates, the number of transcripts (subset ‘c’ 2323), and proteins (subset …
Visualization of functional categories of the 544 proteins that differed by genotype. Four levels of functional tree consist of number of polygons. The areas of each polygon reflect genotypic …
Gene families of proteins regulated by PKA ranged from transcription regulators, kinases, peptidases and other enzymes, transmembrane receptors, ion channels and other gene families. Canonical …
(A) Pathway for catecholamine synthesis and breakdown; (B) Myocardial catecholamine levels (N=12 for each group, Data are presented as Mean± SEM. The statistical significance is indicated by * p<0.05…
A schematic of growth and metabolism circuitry based on signals derived from bioinformatic analyses of the transcriptome and proteome and on selected WBs. Catabolism of glucose is markedly increased …
Data are presented as Mean± SEM. The statistical significance is indicated by * p<0.05, ** p<0.01, *** p<0.001, (two-tailed t test).
Raw unedited blot images and uncropped blot images of AKT2, GAPDH, GPD1, GSK3b and HK1.
Raw unedited blot images and uncropped blot images of LDHA, PDHA1, PDK1, PGD, PIK3CA and PTEN.
Data are presented as Mean± SEM. The statistical significance is indicated by * p<0.05, **** p<0.0001 (two-tailed t test).
The pathways/specific targets and the effector functions/outcomes culminating from the regulation of the components within the circuits in the pathways are represented according to published …
Negative feedback adaptations on AC/PKA signaling.Data are presented as Mean± SEM. The statistical significance is indicated by * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 (two-tailed t test).
Raw unedited blot images and uncropped blot images of PDE4B, PDE4D, PP1, PP2A, PP2B.
Raw unedited blot images and uncropped blot images of PP2B, DARPP-32, PDE3A, PDE4A.
Data are presented as Mean± SEM. The statistical significance is indicated by * p<0.05, ** p<0.01 (two-tailed t test).
Raw unedited blot images and uncropped blot images of Caspase3, Cleaved Caspase3, Cleaved PARP, pFoxO1 (Ser256), GAPDH, HIF1α, JAK2 (Tyr1007/Tyr1008), pCREB (Ser133), pJNK (Thr183/Tyr185), pSTAT3 (Tyr705), total CREB, total FoxO1, total JAK2, total JNK, and total STAT3.
The statistical significance is indicated by * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 (two-tailed t test).
Raw unedited blot images and uncropped blot images of ACE2, AGTR1, AGTR2, and MAS1.
The statistical significance is indicated by * p<0.05, ** p<0.01 (two-tailed t test).
Raw unedited blot images and uncropped blot images of CALR and CNX.
The statistical significance is indicated by * p<0.05, ** p<0.01.
The statistical significance is indicated by * p<0.05.
Data are presented as Mean± SEM (two-tailed t test).
List of: Echo parameters, transcripts and proteins, canonical pathways, cardiovascular disease-related functions within the LV transcriptome and proteome of TGAC8 and WT, RT-qPCR primers and a antibodies used in current study.
(a) List of Echo parameters recorded in TGAC8 heart vs WT. (b) A Listing of all 11810 identified transcripts. (c) A listing of 2323 transcripts that significantly differed in expression by genotype. (d) A listing of all 6834 identified proteins. (e) A listing of 2184 proteins that significantly differed in expression by genotype. (f) A listing of canonical pathways in IPA analysis of the total number of transcripts. (g) A listing of canonical pathways in IPA analysis of the total number of proteins that were differentially enriched or activated in TGAC8 vs WT. (h) A listing of molecules with indication of involvement in number of pathways. (i) A listing of 544 molecules of which both transcripts and proteins differed by genotype. (j) A listing of canonical pathways that were differentially enriched by genotype in IPA analysis of 544 transcripts and proteins. (k) A Complete list of downstream effects of enriched canonical signaling pathways in TGAC8, depicted on Figure 10. (l) RT-qPCR analysis of selected transcripts related to G-protein Coupled Receptor Signaling, that differed by genotype in RNASEQ. (m) Transcripts and proteins involved in Cell Cycle/Cell Proliferation, and Growth and Developmental Circuits that differed between TGAC8 vs WT. (n) IPA representation of top cardiovascular disease-related functions within the LV transcriptome of TGAC8 and WT. (o) IPA representation of top cardiovascular disease-related functions within the LV proteome of TGAC8 and WT. (p) Primers used in RT-qPCR analyses. (q) Antibodies used in WB analyses, antibody arrays and immunostaining.