In a separate cohort of rats (n=8 archaerhodopsin T [ArchT]; n=8 green fluorescent protein [GFP]), (a) optogenetic inhibition was applied upon confinement to the neutral box of the runway task, after object exploration but prior to goal box entry (i.e., choice behavior). (b) All animals demonstrated object-outcome associations by acquisition test 2. (c–d) PRC inhibition significantly increased time spent in the goal box and reduced the number of retreats in the high conflict test. (e) There was no effect of PRC inhibition on AA behavior in the neutral test. (f) Similar to the high conflict test, PRC inhibition increased time spent in the goal box in both low conflict tests. (g–h) PRC inhibition did not impact the number of entries or retreats in the App+Neu or Av+Neu low conflict tests, although there was a main effect of valence, with a greater number of entries for App+Neu and more retreats for Av+Neu. (i–j) PRC-inhibited rats spent more time by the sucrose dispenser, as measured by total time or proportion of time, in the high and low conflict tests but not the no conflict test. All figures show mean values ± SEM. Three-way ANOVA was conducted for all data shown shown except for data in panels c-e and i-j, which were subjected to two-way ANOVA. Significant interactions were followed up with post-hoc tests with Bonferroni correction. ***p<0.001, **p<0.01, *p<0.05.