Male rodent perirhinal cortex, but not ventral hippocampus, inhibition induces approach bias under object-based approach-avoidance conflict
- Department of Psychology (Scarborough), University of Toronto, Canada
- Rotman Research Institute, Baycrest Centre, Canada
- Department of Cell and Systems Biology, University of Toronto, Canada
Figures
Figure 1
Object approach-avoidance (AA) conflict runway task.
Rats expressing AAV1-CaMKIIa-ArchT-GFP (archaerhodopsin T [ArchT]) or AAV8-CAMKIIa-GFP (green fluorescent protein [GFP]) in the perirhinal cortex (PRC, a) underwent object cue conditioning to learn …
Figure 2 with 1 supplement
Impact of perirhinal cortex (PRC) inhibition on object approach-avoidance (AA) conflict runway task performance.
(a) Rats (n=10 archaerhodopsin T [ArchT]; n=12 green fluorescent protein [GFP]) underwent a series of tests conducted in extinction: high conflict (APP+AV objects), neutral (NEU+NEU), and low …
Figure 2—figure supplement 1
Additional perirhinal cortex (PRC) rat data for the approach-avoidance (AA) conflict runway task.
(a) PRC inhibition did not impact rats’ goal box latency to enter (LTE) all compartments (box) of the runway in the high conflict test. (b) Exploration of appetitive (APP) and aversive (AV) objects …
Figure 3 with 1 supplement
Impact of perirhinal cortex (PRC) inhibition on ‘no conflict’ approach-avoidance (AA) conflict runway task performance.
(a) A subset of rats (n=8 archaerhodopsin T [ArchT]; n=8 green fluorescent protein [GFP]) underwent a no conflict recombination task, in which they first learned a new set of appetitive (APP) or …
Figure 3—figure supplement 1
Additional perirhinal cortex (PRC) rat data for the approach-avoidance (AA) no conflict recombination task.
(a–b) PRC inhibition had no effect on latency to enter (LTE) in the appetitive or aversive no conflict recombination tests. (c–d) Exploration of APP and AV objects did not differ during the no …
Figure 4
Perirhinal cortex (PRC) inhibition increases reward location approach behavior.
(a) Heatmap plots for the high conflict test. (b–c) PRC-inhibited rats spent more time by the sucrose dispenser, as measured by total time or proportion of time, in the high and low conflict tests …
Figure 5 with 1 supplement
Perirhinal cortex (PRC) inhibition prior to the choice period of the runway task.
In a separate cohort of rats (n=8 archaerhodopsin T [ArchT]; n=8 green fluorescent protein [GFP]), (a) optogenetic inhibition was applied upon confinement to the neutral box of the runway task, …
Figure 5—figure supplement 1
Additional perirhinal cortex (PRC) rat data with optogenetic inhibition during the choice period for the approach-avoidance (AA) conflict runway task.
(a) PRC inhibition did not impact rats’ latency to enter (LTE) the object, neutral, and goal boxes in the high conflict test. (b) Exploration of App and Av objects did not differ during the high …
Figure 6
Impact of perirhinal cortex (PRC) inhibition on object approach-avoidance (AA) conflict shuttle box task performance.
(a) Timeline of paradigm. (b) Rats (n=7 archaerhodopsin T [ArchT]; n=8 green fluorescent protein [GFP]) first learned to stay to receive a reward when exposed to an appetitive object pair, and to …
Figure 7
Perirhinal cortex (PRC) inhibition during the choice period of the object approach-avoidance (AA) conflict shuttle box task.
(a–b) All rats (n=8 archaerhodopsin T [ArchT]; n=8 green fluorescent protein [GFP]) demonstrated intact acquisition of AA behavior. (c–d) PRC inhibition led to an increased escape latency in the …
Figure 8
No effect of perirhinal cortex (PRC) inhibition on contextual approach-avoidance (AA) conflict behavior.
(a) PRC rats (n=10 archaerhodopsin T [ArchT]; n=8 green fluorescent protein [GFP]) underwent a contextual AA task known to be ventral hippocampus (vHPC)-dependent, in which they first learned the …
Figure 9
No effect of ventral CA3 (vCA3) inhibition on object approach-avoidance (AA) conflict runway task performance.
(a) Rats injected with archaerhodopsin T (ArchT) (n=9) or green fluorescent protein (GFP) (n=6) in the vCA3 underwent the object runway task. (b) Both groups learned the appetitive (App), aversive …
Figure 10
No impact of ventral hippocampus (vHPC) inhibition on object approach-avoidance (AA) conflict shuttle box task performance.
(a–b) Both archaerhodopsin T (ArchT) (n=9) and green fluorescent protein (GFP) ventral CA3 (vCA3) (n=6) rats demonstrated intact acquisition of AA behavior on the shuttle box task. (c–d) vCA3 …
Figure 11
Effect of optogenetic inhibition on perirhinal cortex (PRC)- or ventral CA3 (vCA3)-dependent control tasks and cFos expression.
(a) PRC inhibition disrupted the ability of rats to discriminate a novel and familiar object on the novel object recognition task. (b) vCA3 inhibition increased time spent in the open arm of the …
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Rattus norvegicus) | Long-Evans rats, male, 2–6 months old | Charles River | Cat#2308852, RRID:RGD_2308852 | |
| Antibody | Rb Anti-c-Fos rabbit polyclonal | Synaptic Systems | Cat# 226 003, RRID:AB_2231974 | TSA-IHC (1:5000) |
| Antibody | Peroxidase AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L), donkey polyclonal | Jackson ImmunoResearch | Cat# 711-036-152, RRID: AB_2340590 | TSA-IHC (1:500) |
| Recombinant DNA reagent | pAAV- CaMKIIa-ArchT-GFP | Addgene/Ed Boyden | Cat# 99039-AAV1, RRID: Addgene_99039 | Inhibitory opsin |
| Recombinant DNA reagent | pAAV-CaMKIIa-GFP | Addgene/Bryan Roth | Cat# 50469-AAV8, RRID: Addgene_50469 | Control virus |
| Software, algorithm | SPSS | IBM | https://www.ibm.com/spss | Version 26 |
| Software, algorithm | Ethovision XT | Noldus Information Technology | https://www.noldus.com/ethovision-xt | Animal tracking software/ hardware |
| Software, algorithm | Prism | GraphPad | https://www.graphpad.com/ | Version 8 |
| Other | NHS-Rhodamine | Thermo Fisher Scientific | Cat# 46406 | Rhodamine-based dye; TSA-IHC (1:500) |
