Neural underpinning of a respiration-associated resting-state fMRI network
- The Neuroscience Graduate Program, The Huck Institutes of the Life Sciences, The Pennsylvania State University, United States
- Center for Neurotechnology in Mental Health Research, The Pennsylvania State University, United States
- Department of Biomedical Engineering, The Pennsylvania State University, United States
Figures
Figure 1 with 2 supplements
Simultaneous recordings of fMRI, electrophysiology and respiration signals in rats.
(A) Experimental design—simultaneous measurement of the resting-state fMRI (rsfMRI), electrophysiology, and respirational signals. Top: anterior cingulate cortex (ACC) seedmap; bottom left: …
Figure 1—figure supplement 1
Representative image confirming the electrode location in the anterior cingulate cortex.
Figure 1—figure supplement 2
Removal of MRI artifacts from the electrophysiology signal.
(A) An example of raw electrophysiology signals before the denoising of MRI artifacts; (B) an example of the MRI artifact template. (C) Local field potential (LFP) after MRI artifact denoising.
Figure 2 with 2 supplements
Phase-locking relationship between slow respiration variations and neural activity.
(A) The relationship between respiration and neural activity. (B) Respiration–local field potential (LFP) coherence from one sample scan. (C) Respiration–LFP coherence averaged across all scans. (D) …
Figure 2—figure supplement 1
Electrophysiology results obtained using the differential subtraction method.
(A) Exemplar electrophysiology signals from two separate channels: channel 1 (top) and channel 10 (middle), as well as the subtracted signal between the two channels (bottom). Results in panels B–D …
Figure 2—figure supplement 2
Respiration volume per time (RVT)–gamma power coherence in individual animals in the lightly sedated state.
Figure 3 with 2 supplements
Gamma power is associated with the resting-state fMRI (rsfMRI) signal.
(A) The relationship between neural activity and rsfMRI signal. (B) Top: exemplar gamma-band power in the anterior cingulate cortex (ACC); middle: estimated blood-oxygen-level-dependent (BOLD) …
Figure 3—figure supplement 1
Gamma power–resting-state fMRI (rsfMRI) correlation maps in individual animals in the lightly sedated state.
Figure 3—figure supplement 2
Difference of RSFC maps before and after respiration volume per time (RVT) regression.
(A) Anterior cingulate cortex (ACC) (right side) seedmap after RVT regression; (B) difference of right ACC seedmaps before and after RVT regression; (C) gamma-band power-derived correlation map …
Figure 4 with 3 supplements
Correlation between slow variations of respiration and resting-state fMRI (rsfMRI) signal.
(A) The relationship between respiration and rsfMRI signals. (B, C) Voxel-wise correlations between the respiration volume per time (RVT) and rsfMRI signals. (B) Unthresholded correlation map …
Figure 4—figure supplement 1
Respiration volume per time (RVT)–resting-state fMRI (rsfMRI) correlation maps in individual animals in the lightly sedated state.
Figure 4—figure supplement 2
Voxel-wise correlations between the resting-state fMRI (rsfMRI) signal and RETROICOR regressor with the regression of the white matter and ventricle signals in (A) light sedation and (B) isoelectric state.
Voxel-wise correlations between the rsfMRI signal and RETROICOR regressor without the regression of the white matter and ventricle signals in (C) light sedation and (D) isoelectric state.
Figure 4—figure supplement 3
Voxel-wise correlations between the estimated time course of respirational artifacts and resting-state fMRI (rsfMRI) signals.
(A) Respiratory response function. Voxel-wise correlations between the estimated time course of respirational artifacts and rsfMRI signals without (B) and with (C) the regression of the white matter …
Figure 5 with 1 supplement
Respiration–resting-state fMRI (rsfMRI) relationship at the isoelectric state.
(A) Determining the relationship between slow respiration variations and the rsfMRI signal after silencing the brain-wide neural activity. (B) Silencing neural activity at the isoelectric state …
Figure 5—figure supplement 1
Respiration volume per time (RVT)–resting-state fMRI (rsfMRI) correlation maps in individual animals in the isoelectric state.
Author response image 1
Left, voxel-wise correlations between the estimated time course of respirational artifact, estimated by convolving RVT with the respiration response function, and rsfMRI signals at the isoelectric state.
The same artifactual pattern during light sedation is shown in Figure 4 —figure supplement 3. Middle, statistical comparison of the respiration artifactual patterns between light sedation and …
Author response image 2
