Gene (Mus musculus) | Lrg1 | NCBI | NM_029796.2 | |
Strain, strain background (Mus musculus) | C57BL/6 J | The Jackson Laboratory | Cat #000664, RRID:IMSR_JAX:000664 | |
Strain, strain background (Mus musculus) | BKS.Cg-Dock7m +/+Leprdb/J | The Jackson Laboratory | Cat# 000642, RRID:IMSR_JAX:000642 | |
Strain, strain background (Mus musculus) | LRG1-KO in C57BL/6 J background | This Study | | Produced by the CRISPR and Genome Editing Center (Rockefeller University) |
Strain, strain background (Escherichia coli) | NEB Stable | NEB | Cat# C3040 | Competent cells |
Strain, strain background (Escherichia coli) | BJ5183-AD-1 cells | Agilent | Cat# 200157 | Electroporation competent, recombination proficient cells carrying the pAdEasy-1 plasmid |
Strain, strain background (Escherichia coli) | XL-10 Gold ultracompetent cells | Agilent | Cat# 200314 | Competent cells |
Strain, strain background (Adenovirus) | Ad-eGFP | This study; protocol adapted from Luo et al., 2007 | | Produced in Paul Cohen lab (Rockefeller University) |
Strain, strain background (Adenovirus) | Ad-LRG1-FL | This study; protocol adapted from Luo et al., 2007 | | Produced in Paul Cohen lab (Rockefeller University) |
Strain, strain background (AAV8) | AAV8-eGFP | This study | | Produced by Penn Vector Core (University of Pennsylvania). |
Strain, strain background (AAV8) | AAV8-LRG1-FL | This study | | Produced by Penn Vector Core (University of Pennsylvania). |
Cell line (Homo-sapiens) | HEK293A | Invitrogen | Cat# R70507 | |
Antibody | Anti-LRG1 (rabbit polyclonal) | Sigma-Aldrich | Cat# HPA001888, RRID:AB_1079276 | Western blot (1:5000) |
Antibody | Anti-CFD/Adipsin (sheep polyclonal) | R and D Systems | Cat# AF5430, RRID:AB_1655868 | Western blot (1:1000) |
Antibody | Anti-Cytochrome c (rabbit monoclonal) | Cell Signaling Technology | Cat# 11940, RRID:AB_2637071 | Western blot (1:1000) |
Antibody | Anti-FLAG M2 Magnetic Beads (mouse monoclonal) | Sigma-Aldrich | Cat# M8823, RRID:AB_2637089 | Immunoprecipitation |
Antibody | Anti-Cleaved Caspase-3 (Asp175) (rabbit polyclonal) | Cell Signaling Technology | Cat# 9661, RRID:AB_2341188 | WB (1:1000) |
Antibody | Anti-Caspase-3 (rabbit polyclonal) | Cell Signaling Technology | Cat# 9662, RRID:AB_331439 | WB (1:1000) |
Antibody | Anti-IRS-1 (rabbit polyclonal) | Cell Signaling Technology | Cat# 2382, RRID:AB_330333 | WB (1:500) |
Antibody | Anti-β-actin (rabbit polyclonal) | GeneTex | Cat# GTX109639, RRID:AB_1949572 | WB (1:20000) |
Antibody | Anti-vinculin (rabbit polyclonal) | Cell Signaling Technology | Cat# 4650, RRID:AB_10559207 | WB (1:1000) |
Peptide, recombinant protein | Recombinant Mouse M-CSF | Biolegend | Cat# 576406 | |
Peptide, recombinant protein | Recombinant human LRG1 | R and D Systems | Cat# 7890-LR | |
Peptide, recombinant protein | Recombinant human TNFα | R and D Systems | Cat# 210-TA-020/CF | |
Peptide, recombinant protein | Cytochrome c from equine heart | Sigma-Aldrich | Cat# C2506 | |
Commercial assay or kit | Click-iT Protein Enrichment Kit | Invitrogen | Cat# C10416 | |
Sequence-based reagent | qPCR primers | This paper | | Supplementary file 3 |
Sequence-based reagent | pAdTrack-CMV | Addgene | RRID:Addgene_16405 | |
Sequence-based reagent | pENN.AAV.CB7.CI.eGFP.WPRE.rBG | Addgene | RRID:Addgene_105542 | |