(a) Sagittal view of the PVT (red) illustrating the dissection target location. (b) Distribution of nuclei from four samples across all cell types. (c) The uniform manifold approximation and …
(a–c) Violin plots depicting the percent mitochondrial RNA (a), number of UMIs (b), and the number of genes (c) detected by sample. (d) The UMAP plot of the same nuclei from Figure 1c is labeled by …
(a) The uniform manifold approximation and projection (UMAP) plot of 9845 neuronal nuclei shows nine clusters. (b) Feature plots of representative gene expression of markers from different clusters. …
(a) The uniform manifold approximation and projection (UMAP) plot of 5737 PVT neuronal nuclei shows five clusters. (b) Phylogenetic tree depicting cluster relationships based on the distance between …
(a) The uniform manifold approximation and projection (UMAP) plot of 4067 paraventricular nucleus of the thalamus (PVT) neuronal nuclei shows five clusters. (b) Feature plot of Drd2 expression …
(a) RNA in situ hybridization in anterior PVT (white dotted outline) with closeup insert (right, yellow square) labeling one gene marker from each subtype: Esr1 (light blue), Col12a1 (red), Npffr1 …
(a) Dendrogram depicting Euclidian distance between cluster types based on gene transcript expression. (b–c) Location of positive cells expressing Esr1 (blue square), Col12a1 (black circle), and Drd3…
(a–b) Location of Esr1 (black) or Drd2 (red) positive cells in aPVT (a) or pPVT (b) from PVT1 subtype. (c) Venn diagram of cell count and percent overlap between Esr1- and Drd2-positive cells. (d-e) …
(a) In situ hybridization of Esr1 (green), Drd2 (magenta), and DAPI (blue) in pPVT (white outline). (b–d) Yellow square insert from (a). (e) In situ hybridization of Col12a1 (green), Pde3a …
(a) Dot plot depicting neuromodulator receptor gene expression across five PVT subtypes. (b) Dot plot depicting ion channel gene expression across five PVT subtypes. (c) Dot plot depicting …
(a) The uniform manifold approximation and projection (UMAP) plot of 7709 cells or nuclei from combined datasets annotated by cluster identity. (b) The same cells/nuclei are separated by the study …
(a) The uniform manifold approximation and projection (UMAP) plot of 11,817 cells or nuclei from combined datasets annotated by cluster identity. (b) The same cells/nuclei are separated by the study …
(a) Representative image of the injection site of green fluorescent retrobeads in the prelimbic cortex (PL). (b–e) Representative image of the localization of retrobeads (green) and Col12a1 mRNA …
(a–c) Representative image of the injection site in the prelimbic cortex (PL) of retrograde tracer CTB647 (magenta) and anterograde tracer PHAL (green); Scale bar 1 mm. (d-f) Representative image of …
(a) Schematic of Target Search tool used to identify experiments where projections from the PVT to Auditory (AUD) and Visual (VIS) areas were detected (Mouse Brain Connectivity Atlas of the Allen …
Lists of differential gene expression for snRNA-seq data and conserved marker analysis from the integrated dataset.