Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone
- University of Pennsylvania, United States
- Children's Hospital of Philadelphia, United States
- The University of Texas Health Science Center at San Antonio, United States
Abstract
Colony stimulating factor 1 (Csf1) is an essential growth factor for osteoclast progenitors and an important regulator for bone resorption. It remains elusive which mesenchymal cells synthesize Csf1 to stimulate osteoclastogenesis. We recently identified a novel mesenchymal cell population, marrow adipogenic lineage precursors (MALPs), in bone. Compared to other mesenchymal subpopulations, MALPs expressed Csf1 at a much higher level and this expression was further increased during aging. To investigate its role, we constructed MALP-deficient Csf1 CKO mice using AdipoqCre. These mice had increased femoral trabecular bone mass, but their cortical bone appeared normal. In comparison, depletion of Csf1 in the entire mesenchymal lineage using Prrx1Cre led to a more striking high bone mass phenotype, suggesting that additional mesenchymal subpopulations secrete Csf1. TRAP staining revealed diminished osteoclasts in the femoral secondary spongiosa region of Csf1 CKOAdipoq mice, but not at the chondral-osseous junction nor at the endosteal surface of cortical bone. Moreover, Csf1 CKOAdipoq mice were resistant to LPS-induced calvarial osteolysis. Bone marrow cellularity, hematopoietic progenitors, and macrophages were also reduced in these mice. Taken together, our studies demonstrate that MALPs synthesize Csf1 to control bone remodeling and hematopoiesis.
Data availability
Pre-aligned scRNA-seq matrix files were acquired from previously published dataset GEO GSE145477 and snRNA-seq matrix files were from GSE176171 (mouse), human scRNA-seq matrix files were acquired from EMBL-EBI E-MTAB-9139 (human).All data are available as source data files with submission.
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Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment.NCBI Gene Expression Omnibus, GSE145477.
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A single-cell atlas of human and mouse white adipose tissue.NCBI Gene Expression Omnibus, GSE176171.
Article and author information
Author details
Funding
National Institute on Aging (R01AG069401)
- Ling Qin
National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR078650)
- Ling Qin
National Institute of Arthritis and Musculoskeletal and Skin Diseases (R00AR067283)
- Nathanial Dyment
National Institute on Aging (R01AG045040)
- Jean X Jiang
Welch Foundation (AQ-1507)
- Jean X Jiang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Mei Wan, Johns Hopkins University, United States
Ethics
Animal experimentation: All animal work performed in this report was approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Pennsylvania under Protocol 806887. University Laboratory Animal Resources (ULAR) of the University of Pennsylvania is responsible for the procurement, care, and use of all university-owned animals as approved by IACUC. Animal facilities in the University of Pennsylvania meet federal, state, and local guidelines for laboratory animal care and are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care International.
Version history
- Received: July 22, 2022
- Preprint posted: July 29, 2022 (view preprint)
- Accepted: February 3, 2023
- Accepted Manuscript published: February 13, 2023 (version 1)
- Version of Record published: March 10, 2023 (version 2)
Copyright
© 2023, Zhong et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone
eLife 12:e82112.
https://doi.org/10.7554/eLife.82112
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