Enhanced single RNA imaging reveals dynamic gene expression in live animals
- Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, China
- International Biomedicine-X research center of the Second Affiliated Hospital, Zhejiang University, China
- Center for Stem Cell and Regenerative Medicine and Department of Burn and wound repair of the Second Affiliated Hospital, Zhejiang University School of Medicine, China
Figures
Figure 1 with 6 supplements
Live-cell imaging of β-ACTIN mRNA with the MS2-based signal amplification with the Suntag system.
(A) Schematic of the classical MS2-MCP system and the MS2-based signal amplification with the Suntag system. (B) Representative images of β-ACTIN-8xMS2 mRNA in live HeLa cells. The sfGFP …
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Figure 1—source data 1
qRT-PCR (Quantitative Reverse Transcription PCR), number of foci detected by smFISH and MASS, signal-to-noise ratio, velocity, and intensity of the foci of mRNA detected in HeLa cells.
- https://cdn.elifesciences.org/articles/82178/elife-82178-fig1-data1-v3.xlsx
Figure 1—figure supplement 1
Labeling of β-ACTIN-8xMS2 mRNA by smFISH and MASS with the tdMCP-24xSuntag in HeLa cells.
(A) Schematic of probes targeting the β-ACTIN-8xMS2 tagged mRNA. Probe-1: against the MS2 stem-loop; probe-2: against the linker region. (B) Representative confocal images of β-ACTIN-8xMS2 labeled …
Figure 1—figure supplement 2
MASS did not affect the stability of mRNAs.
(A) Representative confocal images of β-ACTIN-8xMS2 V1 or β-ACTIN-8xMS2 V7 mRNAs labeled by MASS with tdMCP-24xSuntag. (B) Representative confocal images of C-MYC-8xMS2, HSPA1A-8xMS2, and KIF18B-8xMS…
Figure 1—figure supplement 3
MASS did not affect mRNA subcellular localization.
(A) Confocal image of β-ACTIN-8xMS2 mRNA in live HeLa cells. sfGFP foci are shown. Constructs of β-ACTIN-8xMS2, MCP-24xSuntag, and scFv-sfGFP were cotransfected into HeLa cells. Images were taken 12 …
Figure 1—figure supplement 4
MASS has a higher signal-to-noise ratio than the conventional 24xMS2 method.
(A, C, E, G) Confocal images of β-ACTIN-24xMS2 mRNAs labeled by the conventional 24xMS2 system and β-ACTIN-8xMS2 mRNAs labeled by MASS with tdMCP-6xSuntag, tdMCP-12xSuntag, or tdMCP-24xSuntag. …
Figure 1—figure supplement 5
MASS with tdMCP-6xSuntag did not affect the speed of mRNA movement.
(A, B) Distribution of the velocity of mRNA movement of β-ACTIN-24xMS2 mRNAs labeled by the conventional 24xMS2 system and β-ACTIN-8xMS2 mRNAs labeled by MASS with tdMCP-6xSuntag, tdMCP-12xSuntag, …
Figure 1—figure supplement 6
MASS labels mRNAs with a higher intensity than the conventional 24xMS2 imaging system.
(A, B) As Figure 1—figure supplement 5, but the distribution of the average intensity of each spot was shown. (C) Mean intensity of the average intensity of each spot in (A). Bars indicate mean ± …
Figure 2 with 5 supplements
Live imaging of endogenous mRNAs in the epidermis of C. elegans using the MS2-based signal amplification with the Suntag system.
(A) Schematic of the strategy for live imaging of endogenous mRNAs in the epidermis of C. elegans. (B) Representative images of endogenous C42D4.3-8xMS2 mRNA in the epidermis of live C. elegans …
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Figure 2—source data 1
qRT-PCR (Quantitative Reverse Transcription PCR), number, and size of foci of mRNAs detected by MASS in C. elegans.
- https://cdn.elifesciences.org/articles/82178/elife-82178-fig2-data1-v3.xlsx
Figure 2—figure supplement 1
Live imaging of cdc42 mRNA in C. elegans using MS2-based signal Amplification with Suntag System.
Representative images of cdc42-8xMS2 mRNA in live C. elegans. sfGFP fluorescence signals are shown. Left panel: Constructs of cdc42-8xMS2, MCP-24xSuntag, and scFv-sfGFP were coexpressed in the …
Figure 2—figure supplement 2
Live imaging of endogenous mRNA in the epidermis of C. elegans using the MS2-based signal amplification with the Suntag system.
(A) Representative images of endogenous mai-1-8xMS2 mRNA in the epidermis of live C. elegans using the strategy described in Figure 2A. Left: mai-1 without 8xMS2. Right: mai-1 with 8xMS2. Images …
Figure 2—figure supplement 3
Fast activation and spreading of endogenous gene expression in the epidermis of C. elegans.
Time-lapse imaging of endogenous C42D4.3-8xMS2 mRNA in the epidermis of live C. elegans before and after wounding. White dashed circles indicate the wound sites. Scale bar, 10 μm.
Figure 2—figure supplement 4
Treatment of Actinomycin D blocks the formation of C42D4.3 mRNA foci.
Time-lapse imaging of endogenous C42D4.3-8xMS2 mRNA in the epidermis of live C. elegans before and after wounding. C. elegans were treated with Actinomycin D (30 μM) for 3 hr before wounding. White …
Figure 2—figure supplement 5
Foci of endogenous C42D4.3-8xMS2 mRNAs showed a larger size than that of BFP-8xMS2 mRNAs.
(A) Time-lapse imaging of BFP-8xMS2 mRNA in the epidermis of C. elegans using MASS with MCP-24xSuntag. Scale bar, 10 μm. (B) Quantification of the puncta size of the BFP-8xMS2 mRNA foci for 5 min, …
Videos
Video 1
Time-lapse imaging of β-ACTIN-8xMS2 mRNA in live HeLa cells.
The sfGFP foci of the β-ACTIN-3′UTR-8xMS2 mRNA localize to the lamellipodia in HeLa cells. The white dashed line demarcates the cell. The white arrow indicates lamellipodia. Time interval, 2 s. …
Video 2
Time-lapse imaging of sfGFP foci of β-ACTIN-8xMS2 mRNA with a time interval of 1 s in HeLa cells.
Scale bar, 5 μm.
Video 3
Time-lapse imaging showing fusion events of sfGFP foci of β-ACTIN-8xMS2 mRNA in HeLa cells where small sfGFP spots (white and yellow arrows) fuse into a single more prominent spot.
Time interval, 2 s. Scale bar, 5μm.
Video 4
Time-lapse imaging showing fission events of sfGFP foci of β-ACTIN-8xMS2 mRNA in HeLa cells where large sfGFP foci split into smaller spots (white and yellow arrows).
Time interval, 1 s. Scale bar, 1 μm.
Video 5
Time-lapse imaging showing transient interactions of an sfGFP spot (yellow arrow) between two spots (white arrows) of β-ACTIN-8xMS2 mRNA in HeLa cells.
Time interval, 2 s. Scale bar, 1 μm.
Video 6
Time-lapse imaging in HeLa cells showing an sfGFP spot of β-ACTIN-8xMS2 mRNA showing no movement over a 10-s period.
Time interval, 1 s. Scale bar, 1 μm.
Video 7
Time-lapse imaging of endogenous C42D4.3-8xMS2 mRNA dynamics in the epidermis of C. elegans after laser wounding.
Time interval, 5 s. Scale bar, 10 μm.
Video 8
Time-lapse imaging of endogenous C42D4.3-8xMS2 mRNA dynamics in the epidermis of C. elegans after laser wounding.
A different worm was shown. Time interval, 5 s. Scale bar, 10 μm.
Video 9
Time-lapse imaging of endogenous mai-1-8xMS2 mRNA dynamics in the epidermis of C. elegans after laser wounding.
Time interval, 2 s. Scale bar, 10 μm.
Video 10
Time-lapse imaging showing fusion events of sfGFP foci of endogenous C42D4.3-8xMS2 mRNA in the epidermis of C. elegans where small sfGFP spots (white arrows) fused into a more prominent spot.
Time interval, 5 s. Scale bar, 5 μm.
Video 11
Time-lapse imaging of exogenous BFP-8xMS2 mRNA dynamics in the epidermis of C. elegans.
Time interval, 0.5 s. Scale bar, 10 μm.
