Abstract
Imaging endogenous mRNAs in live animals is technically challenging. Here we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1,300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool, we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans.
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Videos 1-11 contain source data for Figures.Figure 1-Source data 1 contains source data for statistical analysis in cells.Figure 2-Source data 1 contains source data for statistical analysis in C. elegans.
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Funding
National Natural Science Foundation of China (31972891)
- Suhong Xu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2023, Hu et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Enhanced single RNA imaging reveals dynamic gene expression in live animals
eLife 12:e82178.
https://doi.org/10.7554/eLife.82178
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