Functional cell types in the mouse superior colliculus
- Division of Biology and Biological Engineering, California Institute of Technology, United States
- Chinese Institute for Brain Research, China
- Tianqiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, United States
Figures
Figure 1
Two-photon imaging reveals diverse visual responses in awake mouse superior colliculus.
(A) Schematic of the experimental setup. Mice were head-fixed and free to run on a circular treadmill. Visual stimuli were presented on a screen. Neuronal calcium activity was imaged using …
Figure 2 with 3 supplements
Twenty-four functional cell types in the mouse SC.
(A) Dendrogram of 24 clusters based on their distance in feature space. For each type, this shows the average time course of the neural response to the stimulus panel. Grey: standard deviation. The …
Figure 2—figure supplement 1
Clustering and validating (related to Figure 2).
(A) Temporal features were extracted from responses to five visual stimuli, including chirp, moving bar, expanding black disc and receding white disc, flashed blue or green squares, and flashed …
Figure 2—figure supplement 2
Dendrogram of 24 clusters showing normalized temporal profiles (related to Figure 2).
Figure 2—figure supplement 3
Example responses of single neurons in each type to visual stimuli (related to Figure 2).
Columns 1–6 are time-varying calcium responses to a moving bar, expanding and contracting disks, a "chirp" stimulus with modulation of amplitude and frequency, spots of varying size, and blue and …
Figure 3 with 1 supplement
Functional diversity among different types.
(A) Violin plots or histograms of various response indices: motion selectivity index (MSI), direction selectivity index (DSI), orientation selectivity index (OSI), habituation index (HI), looming …
Figure 3—figure supplement 1
Functional properties of different cell types (related to Figure 3).
(A) Normalized selectivity index (normalized for each column) of functional properties represented by different cell types (see Materials and methods). (B) Pearson’s correlation coefficients of the …
Figure 4 with 2 supplements
Spatial organization of functional cell types.
(A) Anatomical locations of four types of neurons from one sample recording. (B) Averaged density recovery profile (DRP) of five example types for all imaging planes. Error bars denote SEM. (C) …
Figure 4—figure supplement 1
Anatomical organization of functional types (related to Figure 4).
(A) Anatomical locations of different types of neurons in two examples of imaging fields. (B) Number of types that are significantly separated from each reference type (p<0.05, bootstrap analysis). …
Figure 4—figure supplement 2
Receptive field center positions for each functional type (related to Figure 4).
Cell types are indicated by the red number at the corner. Color indicates different mice. Axes represent azimuth (horizontal) and elevation (vertical) in degrees. Red cross indicates median azimuth …
Figure 5
Functional properties in genetically labeled populations.
Figure 6 with 1 supplement
Comparison with functional RGC types.
(A) Weights of different RGC types on each SC type (see Materials and methods). Green dashed line separates Group 1 and Group 2. (B) Plot of mean (triangle) and median (star) OSI versus DSI for 32 …
Figure 6—figure supplement 1
Comparisons between SC neurons and RGCs (related to Figure 6).
(A) Visual responses of RGC types (Baden et al., 2016) and SC types. (B) Prediction error from RGC types to SC types (Equation 28). (C) Histogram of the weights of RGC types to SC types in Figure 6A.…
