Regulation of chromatin microphase separation by binding of protein complexes
- Department of Chemical and Biological Physics, Weizmann Institute of Science, Israel
- Department of Molecular Genetics, Weizmann Institute of Science, Israel
Figures
Figure 1
Spatial organization of RNA Pol II and chromatin.
Representative image of a muscle nucleus of live Drosophila third instar larvae labeled with Histone2B-mRFP (A, red, expressed under endogenous promoter) and with Rpb3-GFP (A’, green, expressed in …
Figure 2
Microphase separation of active and inactive chromatin regions.
Left: Chromatin as a multiblock copolymer with active (green) and inactive blocks (red) comprising and monomers, respectively. Right: Core-shell organization of chromatin. The cores (red area) …
Figure 3
Microphase separation of chromatin in a layer geometry.
The microphase-separated state in the layer geometry is characterized by three regions: The core (red shaded area), which is composed of inactive blocks (red curves) and solvent (not shown), the …
Figure 4
Comparison of the chromatin microphase separation by the binding of protein complexes in layer and spherical geometries.
(A) Single pair of active (green) and inactive blocks (green) in the layer geometry with no (left), few (middle), and many (right) protein complexes bound to a chromatin active block. The swelling …
Figure 5
Regulation of chromatin microphase separation by binding of protein complexes.
(A) Simulation snapshots show a core-shell organization of chromatin. The cores (red area) are composed of inactive blocks (red lines) of chromatin, and the shells (green area) are composed of …
Figure 6
Sketch of chromatin microphase separation in confinement.
Left: Weak binding of protein complexes (yellow) to active chromatin (green) results in larger inactive chromatin domains (red) that wet the nuclear envelope (black) as spherical caps. Right: Strong …
Appendix 1—figure 1
Distribution of RNA Pol II in live Drosophila salivary gland epithelium.
The salivary gland in intact live larvae expressing His2B under endogenous promoter (Red), and Rpb3-GFP under Mef2Gal4 driver imaged under the microscope. (A) His2B-RFP representing H2B-associated …
Tables
Appendix 5—table 1
Characteristics of the flat inactive core and active shell for different regimes effective solvent regime.
The effective second and third virial coefficients are and , respectively. In the table we use the following dimensionless quantities: the extension of the active brush, , the extension of …
| Poor solvent | Good solvent | Theta solvent |
|---|---|---|
