Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells
- Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Switzerland
- University Research Priority Program (URPP), Adaptive Brain Circuits in Development and Learning (AdaBD), University of Zurich, Switzerland
Figures
Figure 1 with 1 supplement
Recombination-induced tag exchange (RITE)-based birthdating of centrosomes.
(A) Centriolin is tagged with the RITE system in human embryonic stem cells (hESCs) so that all Centriolin protein made is tdTomato-tagged. Upon recombination with Cre recombinase, the tdTomato …
Figure 1—figure supplement 1
Centrosome analyses in human embryonic stem cells (hESCs).
(A) hESC electroporated with GFP-Centrin-1 is outlined in white. Centriolin localises to only one of the two centrioles marked by GFP-Centrin-1. Scale bars, 10 μm.
Figure 2 with 1 supplement
Asymmetric inheritance of centrosomes in human forebrain organoids.
(A) Cortical unit of a day 35 forebrain organoid. The ventricular zone is easily identified by densly packed SOX2-positive neural progenitor cells (NPCs) that form a rosette-like structure around …
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Figure 2—source data 1
Raw data related to Figure 2G, H.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig2-data1-v2.xlsx
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Figure 2—source data 2
Raw data related to Figure 2—figure supplement 1C.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig2-data2-v2.xlsx
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Figure 2—source data 3
Raw data related to Figure 2—figure supplement 1D, E.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig2-data3-v2.xlsx
Figure 2—figure supplement 1
Centrosome analyses in organoids.
(A) Scheme showing a timeline of forebrain organoid protocol, with the small molecules used during each part of the protocol. (B) Cross-sections of a ventricle show its three-dimensional, …
Figure 3 with 1 supplement
Randomisation of centrosome inheritance affects neural progenitor cell (NPC) fate.
(A) Scheme showing the timing of electroporation of the shRNA construct in the context of the organoid protocol. (B) Day 40 WT organoids, 5 days post electroporation with either scrambled shRNA or …
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Figure 3—source data 1
Raw data related to Figure 3—figure supplement 1D.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig3-data1-v2.xlsx
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Figure 3—source data 2
Raw data related to Figure 3—figure supplement 1E, F.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig3-data2-v2.xlsx
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Figure 3—source data 3
Raw data related to Figure 3B–D.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig3-data3-v2.xlsx
Figure 3—figure supplement 1
Ninein knockdown in human cells.
(A) Genetic map of the constructs used for the electroporation experiments. shRNA is expressed through the U6 promoter and the H2B-CFP is expressed via the EF-1α promoter. (B) Genetic map of the …
Figure 4
Ninein-shRNA alters retention of the older centrosome in the ventricular zone.
(A) Timing of the retroviral transduction and subsequent recombination in the context of the organoid protocol. (B) Day 24 Centriolin-RITE organoids were infected with retroviral expression of …
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Figure 4—source data 1
Raw data related to Figure 4C, D.
- https://cdn.elifesciences.org/articles/83157/elife-83157-fig4-data1-v2.xlsx
