Structured illumination microscopy (SIM) in mouse flexor digitorum brevis (FDB) muscle (A) and human vastus lateralis muscle (B) of endogenous α-tubulin and GLUT4 (left panel) and 3D reconstruction …
(A) Confocal imaging of α-tubulin (top panel), detyrosinated tubulin (De-Tubulin, bottom panel) and GLUT4-GFP in flexor digitorum brevis (FDB) fibers from muscles expressing GLUT4-GFP. (B) …
Video of live flexor digitorum brevis (FDB) fibers electroporated with GLUT4-GFP (green) and mCherry-Tubulin (magenta) 6 days earlier. The video is played at 10 frames per second with one frame …
Video of GLUT4-GFP at GLUT4-enriched/large structure in live flexor digitorum brevis (FDB) fibers showing spherical and tubular GLUT4-GFP structures that fuse and bud off from the GLUT4-enriched …
(A) Representative time-lapse traces of GLUT4-GFP vesicle tracking in muscle fibers ± insulin (INS, 30 nM) for 15–30 min with or without microtubule network disruption by Nocodazole (Noco, 13 µM) …
Data used for quantification of GLUT4-Stx6 overlap in perinuclear region of mouse flexor digitorum brevis muscle fibers in DMSO medium with and without Noco (13 µM) treatment.
(A) Phosphorylation of Akt Thr308 in GLUT4-GFP-expressing flexor digitorum brevis (FDB) fibers treated ± 30 nM insulin (INS) for 15 min. (B) Polymerized microtubules in GLUT4-GFP-expressing fibers …
Data used for quantification of Figure 2—figure supplement 1A–D, G and raw unedited blots for Figure 2—figure supplement 1A.
Video of live flexor digitorum brevis (FDB) fibers electroporated with GLUT4-GFP 6 days earlier. To facilitate visualization, the video was generated so that moving GLUT4 appears green-red flashing …
(A) 2-Deoxyglucose (2-DG) transport in basal and insulin-stimulated mouse soleus and extensor digitorum longus (EDL) muscles pretreated with Nocodazole (Noco, 13 µM) for the indicated time. (B) …
Data used for quantification of 2-DG transport and glucose clearance and uptake in Figure 3A, B, E, F, polymerized microtubules in Figure 3D and glucose transporter 4 (GLUT4) localization in Figure 3H.
Quantification of protein expression in soleus (A) and extensor digitorum longus (EDL) (B) muscles in the basal and insulin-stimulated state ± Nocodazole (Noco, 13 µM) as indicated. (C) …
Data used for quantification of protein expression and electrochemical glucose sensing in Figure 3—figure supplement 1.
Data used for quantification of Figure 3—figure supplement 1A, B, F, I and raw unedited blots for Figure 2—figure supplement 1A, B.
(A) Schematic overview of L6 muscle cell system to assess GLUT4 surface content. (B) 2-Deoxyglucose (2-DG) transport in basal and insulin-stimulated mouse soleus and extensor digitorum longus (EDL) …
Data used for quantification of glucose transporter 4 (GLUT4) localization and GLUT4 surface content in Figure 4.
Data used for quantification of 2-DG transport and GLUT4 translocation in Figure 4B–D.
(A, B) Quantification and representative immunoblots of protein expression in soleus and extensor digitorum longus (EDL) muscles in the basal and insulin-stimulated state ± 2 hr kinesore (50 µM) …
Data used for quantification of glucose transporter 4 (GLUT4) surface content, protein expression, and glucose uptake in Figure 4—figure supplement 1.
Data used for quantification of Figure 4—figure supplement 1A–C, E–I and raw unedited blots for Figure 4—figure supplement 1A, B, D, I.
(A) Overview of in vitro and in vivo insulin resistance models used. (B) Quantified microtubule-based GLUT4 trafficking in basal, insulin (INS, 30 nM) and insulin + C2 ceramide (C2) (INS + C2, 30 nM …
Data used for quantification of glucose transporter 4 (GLUT4) trafficking and microtubule polymerization in Figure 5B, C, E, F.
(A) Phosphorylation of AktThr308 in GLUT4-GFP-expressing flexor digitorum brevis (FDB) muscle fibers cultured ± 0.5 mM palmitic acid (PA) for 24 hr or 50 µM C2 ceramide (C2) for 2 hr prior to 15 min …
Data used for quantification of protein expression, glucose transporter 4 (GLUT4) trafficking, glucose and insulin tolerance, microtubule polymerization and polymerization directionality in Figure 5—figure supplement 1.
Data used for quantification of Figure 5—figure supplement 1A–F and H–J and raw unedited blots for Figure 5—figure supplement 1A, D.
Video of polymerizing microtubule (MT) tips detected by EB3-GFP in live flexor digitorum brevis (FDB) fibers electroporated with EB3-GFP 6 days earlier. To facilitate visualization, the video was …