Competitive interactions between culturable bacteria are highly non-additive

  1. Amichai Baichman-Kass
  2. Tingting Song
  3. Jonathan Friedman  Is a corresponding author
  1. Hebrew University of Jerusalem, Israel

Abstract

Microorganisms are found in diverse communities whose structure and function are determined by interspecific interactions. Just as single species seldom exist in isolation, communities as a whole are also constantly challenged and affected by external species. Though much work has been done on characterizing how individual species affect each other through pairwise interactions, the joint effects of multiple species on a single (focal) species, remain under explored. As such, it is still unclear how single species effects combine to a community-level effect on a species of interest. To explore this relationship, we assayed thousands of communities of two, three, and four bacterial species, measuring the effect of single, pairs of, and trios of 61 affecting species on six different focal species. We found that when multiple species each have a negative effect on a focal species, their joint effect is typically not given by the sum of the effects of individual affecting species. Rather, they are dominated by the strongest individual-species effect. Therefore, while joint effects of multiple species are often non-additive, they can still be derived from the effects of individual species, making it plausible to map complex interaction networks based on pairwise measurements. This finding is important for understanding the fate of species introduced into an occupied environment, and is relevant for applications in medicine and agriculture, such as probiotics and biocontrol agents, as well as for ecological questions surrounding migrating and invasive species.

Data availability

Sequencing data are provided in Data S1 file, and have been deposited to Genbank under accession codes OP389073-OP389107 and OP412780-OP412788.All data generated or analyzed during this study are available on GitHub, and can be found at https://zenodo.org/badge/latestdoi/534114367.

The following data sets were generated

Article and author information

Author details

  1. Amichai Baichman-Kass

    Institute of Environmental Sciences, Hebrew University of Jerusalem, Rehovot, Israel
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0924-3191
  2. Tingting Song

    Institute of Environmental Sciences, Hebrew University of Jerusalem, Rehovot, Israel
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6740-2085
  3. Jonathan Friedman

    Institute of Environmental Sciences, Hebrew University of Jerusalem, Rehovot, Israel
    For correspondence
    yonatan.friedman@mail.huji.ac.il
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8476-8030

Funding

United States - Israel Binational Science Foundation (2017179)

  • Amichai Baichman-Kass
  • Tingting Song
  • Jonathan Friedman

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2023, Baichman-Kass et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Amichai Baichman-Kass
  2. Tingting Song
  3. Jonathan Friedman
(2023)
Competitive interactions between culturable bacteria are highly non-additive
eLife 12:e83398.
https://doi.org/10.7554/eLife.83398

Share this article

https://doi.org/10.7554/eLife.83398

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