(A) Representative XY view of the basal stem cell layer showing the Kertain14-driven Histone2B-GFP allele in a live mouse. (B) A representative chromatin compaction profile of a single basal stem …
Heterogeneous chromatin compaction independent of interphase cell cycle status.
(A) XZ schematic of the mouse epidermis. The basal stem cell layer (bottom) contains actively cycling cells as well as cells that have committed to differentiation and are actively leaving the …
(A) Representative crops of fixed tissue, single channel stainings of the basal stem cell layer. Genetically encoded K14H2B-GFP signal is shown in green, and immunofluorescent stainings in magenta. …
(A) XZ schematic of the epidermis. The basal stem cell layer is shown with black nuclei and the differentiated (spinous) layer is shown apical with red nuclei. (B) Representative crops of individual …
Chromatin compaction state changes with differentiation status.
(A) Schematic of the inducible genetic system used in Figure 2 to recombine and knock down Srf. (B) Representative crops from the basal stem cell and differentiated layers on days 0 and 6 in …
(A) Time lapse imaging data of a single nucleus crop at 0, 1.5, and 3 hr time points. H2B-GFP fluorescent signal is shown in white. Three, high-intensity chromocenters were chosen and pseudo-colored …
Chromatin compaction state changes slowly over days.
(A) Individual nucleus chromatin compaction profiles from 3 basal stem cell and 3 delaminating/spinning cells at 0-, 1.5-, and 3 hr time points showing little change over this time scale. (B) …
(A) XY schematic of genetic system (K14H2B-GFP; K10rtTA; tetO-Cre; R26LSL-tdTomato) allowing visualization of actively differentiating cells still within the basal stem cell layer (expressing …
Chromatin compaction state begins remodeling upstream of basal delamination.
(A) Visual schematic of the MCP/MS2 system allowing visualization of a targeted gene under endogenous regulation. 24X MS2 repeats were knocked into the 3′UTR of the Keratin-10 locus and chromatin …
Transcription of differentiation gene is highly dynamic and precedes significant chromatin compaction changes.
(A) Representative crops of the differentiated spinous layer (left) and basal stem cell layer (right), as well as magnified inset showing active site of Keratin-10 transcription, and nascent …
Epidermal stem cells undergo incremental changes toward differentiation over 3–4 days (top). During this process, differentiation-committed cells within the basal stem cell layer begin expressing Ker…
A single basal stem cell layer nucleus crop visualizing H2B-GFP intensity. The video first scans through the greyscale, intensity image (white), then through the FIRE LUT intensities shown in Figure …
An XY field-of-view of the upper, basal stem cell layer in which chromatin (H2B-GFP) can be observed to spin. Timelapse is 3 hours long and looped 3 times.