PDO2 contains two major clades of 42 and 30 cells. These clades are characterized by changes in chromosome 7 (triplicated in the first clade and diploid in the second, in accordance with the copy number of roughly 2.75 from bulk sequencing) and chromosome 10 (showing the opposite trend). Few subclonal losses are observed, except for those in chromosome 13 p. In PDO3, the two clades are comprised of 48 and 92 cells, with the second clade containing two subclades of 40 and 52 cells. The largest differences are in chromosomes 1, 4 and 5, 10, and 11. There is an loss of heterozygosity (LOH) region at the start of chromosome 5 in the second clade, in all other chromosomes there are further copy number gains in regions where the first clade already shows amplifications. The two subclades contain differences in chromosomes 1, 4, and 5. PDO6 contains two clades of 49 and 303 cells (the second clade is split into subclades of 158 and 145 cells). The differences in the first split are in chromosomes 3, 4, 6, and 10, in which the extent of amplifications varies, and in chromosome 13, which presents large regions of single copy loss in the second clade. The greatest difference between the subclades is a large triplication of chromosome 2 in the first subclade.