(A) Schematic representation of the rcsD frameshift mutation that occurred during speciation of Y. pestis from its ancestor Y. pseudotuberculosis. rcsDpstb: rcsD from Y. pseudotuberculosis; rcsDpe: r…
(A) Schematic representation of mutations introduced to the putative RBS and start codon of RcsD-Hpt. ATT–312→GGT or ATT–312→ATG indicate that the predicted ATT start codon was mutated to GGT or …
Raw source data for Figure 2D.
(A) Schematic representation of mutations introduced to the putative but non-functional start codons. (B) Crystal violet (CV) biofilm assay using wild type (WT) and ΔrcsD strains complemented by the …
Raw source data for Figure 2—figure supplement 1E.
The original file of the full raw unedited blots and the uncropped blot with the relevant bands clearly labelled as Figure 2—figure supplement 1E.
Raw source data for Figure 2—figure supplement 1F.
The original file of the full raw unedited blots and the uncropped blot with the relevant bands clearly labelled as Figure 2—figure supplement 1F.
(A) Crystal violet (CV) assay of Y. pestis expressing rcsD and its H844A-relevant derivatives. CV assays in panel C of Figure 2—figure supplement 1 and panel A of Figure 2—figure supplement 2 were …
Congo red (CR) pigmentation assay (A and C) and crystal violet (CV) biofilm assay (B and D) the derivatives of Y. pestis KIM6+ (wild type [WT]) and the rcsDpstb substitution strain. prcsF, plasmid …
(A) Crystal violet (CV) biofilm assays using a wild type (WT) (white), ΔrcsB (grey), or rcsB (D56Q) (black) mutant strain, each harbouring pUC19 vectors expressing rcsDpe or rcsDpstb. (B) …
Raw source data for Figure 4B.
The original file of the full raw unedited blots and the uncropped blot with the relevant bands clearly labelled as Figure 4B.
Raw source data for Figure 4D.
The original file of the full raw unedited blots and the uncropped blot with the relevant bands clearly labelled as Figure 4D.
(A) Bacterial burdens in fleas infected with Y. pestis wild type (WT), rcsDpe::rcsDpstb and rcsDN-term strains after 0, 7, and 14 days of infection. (B) Cumulative blockage of fleas after 4 weeks of …
(A–C) Survival of C57BL/6 mice infected with Y. pestis Microtus strain 201 and its derivatives using an infectious dose of 40, 160, and 800 colony-forming units (CFU). (D) Venn diagram of …
(A–D) Clusters of orthologous groups (COG) analysis of RNA-seq gene expression data comparing the ΔrcsB strain and wild type (WT) (A), and rcsDpe::rcsDpstb with WT (B) at 26°C; ΔrcsB with WT (C), …
Acquisition events are indicated by green lightning symbols, loss of genetic material by a red cross, and mutation by blue circles. All comparisons shown are relative to Y. pseudotuberculosis. …
In Y. pseudotuberculosis, full-length RcsD plays a dominant role while the lowly expressed RcsD-Hpt might play a moonlighting function. When Rcs is in ‘off’ state, RcsF is not activated leading to …
Functional rcsA and intact rcsD are encoded by ancestral Y. pseudotuberculosis. Biofilm formation was strongly repressed by the Rcs system due to the presence of RcsA. Y. pseudotuberculosis was …
Strains used in this study.
Plasmids used in this study.
Primers and oligos used in this study.
Differential expression of Y. pestis genes in Y. pestis mutant strains growing at temperatures (26°C or 37°C).
Mutations in Y. pestis during evolution involved in this study.
Putative SD and start codon sites in rcsD in Enterobacteriaceae.