Behavioral dissection of hunger states in Drosophila
- Department of Molecular and Integrative Physiology and Geriatrics Center, Biomedical Sciences and Research Building, University of Michigan, United States
- College of Literature, Science, and the Arts, Biomedical Sciences and Research Building, University of Michigan, United States
- Neuroscience Graduate Program, University of Michigan, University of Michigan, United States
Figures
Figure 1 with 1 supplement
Characterization of feeding micro-behaviors in flies.
(A) Cartoon schematic of setup for filming flies on the Fly Liquid-Food Interaction Counter (FLIC). Frame capture is triggered by interactions of flies with the food source. (B) Frequency of all …
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Figure 1—source data 1
Excel spreadsheet containing source data used to generate Figures 1B-D.
- https://cdn.elifesciences.org/articles/84537/elife-84537-fig1-data1-v1.xls
Figure 1—figure supplement 1
24 hr FLIC recording demonstrating distinct morning and evening feeding peaks.
(A) Representative temporal plot of food interactions on 2% sucrose Fly Liquid-Food Interaction Counter (FLIC) from female Canton-S flies monitored over 24 hr. Each data point represents the binned …
Figure 2
Fly Liquid-Food Interaction Counter (FLIC) signal characteristics distinguish between event types.
(A–C) Representative FLIC signal generated during a visually identified interaction (I), fast feeding (F), or long feeding (L/LQ) event. (D–I) Signal characteristics generated by the FLIC during …
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Figure 2—source data 1
Excel spreadsheet containing source data used to generate Figures 2D-I.
- https://cdn.elifesciences.org/articles/84537/elife-84537-fig2-data1-v1.xls
Figure 3 with 1 supplement
Feeding event durations are increased by hedonic food environments.
(A) Cartoon schematic of event number and event duration (top). Cartoon schematic of hedonic and control food choice environments, and comparisons used to define metrics for homeostatic and hedonic …
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Figure 3—source data 1
Excel spreadsheet containing source data used to generate Figures 3B-M and Figure 3 - Figure Supplement 1A-C.
- https://cdn.elifesciences.org/articles/84537/elife-84537-fig3-data1-v1.xls
Figure 3—figure supplement 1
Hedonic food environments do not elicit increases in total number of events or event durations with yeast but do promote increased volumetric intake.
(A) Consumption-excretion (Con-Ex) measurement of volumetric sucrose consumptions (left panel) and total mass consumed (right panel) in the control vs. hedonic food environments from female Canton-S …
Figure 4
Hedonic feeding is modulated by homeostatic hunger.
(A) Total number of events with sucrose or yeast in the control food choice environment from sated or 24 hr starved female Canton-S flies (two-way ANOVA with Tukey’s post hoc). (B) Total number of …
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Figure 4—source data 1
Excel spreadsheet containing source data used to generate Figures 4A-D.
- https://cdn.elifesciences.org/articles/84537/elife-84537-fig4-data1-v1.xls
Figure 5 with 13 supplements
Hedonic hunger requires distinct mushroom body lobes.
(A) Representative maximum intensity projections from brains of flies expressing Nsyb-GAL4>UAS-CaMPARI exposed to control or hedonic food environments, quantified at right (scale bar = 100 µM, …
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Figure 5—source data 1
Excel spreadsheet containing source data used to generate Figures 5A-G and Figure 5 - Figure Supplements 1A-C.
- https://cdn.elifesciences.org/articles/84537/elife-84537-fig5-data1-v1.xls
Figure 5—figure supplement 1
Mushroom body lobes are not required for homeostatic hunger.
(A–C) Total number of events with sucrose or yeast in the control food choice environment from 24 hr starved female flies expressing UAS-GtACR driven by either (A) MB131B-GAL4 (γ(d);γ(m)), (B) MB008B…
Figure 5—video 1
NsybCaMPARI stacks control environment green, related to Figure 5A–B.
Movies of confocal z-stacks through whole brains of flies carrying Nsyb-Gal4>UAS-CaMPARI (Figure 5—videos 1–6) or MB238Y-GAL4>UAS-CaMPARI (Figure 5—videos 7–12) in control or hedonic food …
Figure 5—video 2
NsybCaMPARI stacks control environment red.
Figure 5—video 3
NsybCaMPARI stacks control environment merge.
Figure 5—video 4
NsybCaMPARI stacks hedonic environment green.
Figure 5—video 5
NsybCaMPARI stacks hedonic environment red.
Figure 5—video 6
NsybCaMPARI stacks hedonic environment merge.
Figure 5—video 7
MBCaMPARI stacks control environment green.
Figure 5—video 8
MBCaMPARI stacks control environment red.
Figure 5—video 9
MBCaMPARI stacks control environment merge.
Figure 5—video 10
MBCaMPARI stacks hedonic environment green.
Figure 5—video 11
MBCaMPARI stacks hedonic environment red.
Figure 5—video 12
MBCaMPARI stacks hedonic environment merge.
Tables
Table 1
Library of feeding micro-behaviors.
| Main event type | Code | Behavior description | |
|---|---|---|---|
| Other (O) | G | Groom | |
| UC | Unknown contact | ||
| Interaction (I) | IF | Front legs touch food | |
| IH | Hind legs touch food | ||
| Fast feeding (F) | F | Single movement of proboscis into food to feed for 1–3 s | |
| FI | Meets requirements for F and I | ||
| Long feeding (L) | L | Proboscis continuously in food while feeding for >4 s | |
| LI | Meets requirements for L and I | ||
| Long/quick feeding (L) | LQ | Proboscis moves in and out quickly while feeding for >4 s |
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | Canton-S | Bloomington Drosophila Stock Center | ||
| Genetic reagent (Drosophila melanogaster) | UAS-CaMPARI | Bloomington Drosophila Stock Center | BDSC #68762 RRID:BDSC_68762 | |
| Genetic reagent (Drosophila melanogaster) | MB238Y-GAL4 | Bloomington Drosophila Stock Center | BDSC #81009 RRID:BDSC_81009 | |
| Genetic reagent (Drosophila melanogaster) | MB131B-GAL4 | Bloomington Drosophila Stock Center | BDSC #68265 RRID:BDSC_68265 | |
| Genetic reagent (Drosophila melanogaster) | MB008B-GAL4 | Bloomington Drosophila Stock Center | BDSC #68291 RRID:BDSC_68291 | |
| Genetic reagent (Drosophila melanogaster) | MB461B-GAL4 | Bloomington Drosophila Stock Center | BDSC #68327 RRID:BDSC_68327 | |
| Genetic reagent (Drosophila melanogaster) | GMR58E02-GAL4 | Bloomington Drosophila Stock Center | BDSC #41347 RRID:BDSC_41347 | |
| Genetic reagent (Drosophila melanogaster) | LexAop2-CsChrimson;UAS-CaMPARI2;GMR57C10-GAL4 | Bloomington Drosophila Stock Center | BDSC #81089 RRID:BDSC_81089 | |
| Genetic reagent (Drosophila melanogaster) | UAS-GtACR | Other | M. Dus (University of Michigan) | |
| Software, algorithm | RStudio | RStudio | RRID:SCR_000432 | |
| Software, algorithm | Fiji | ImageJ | RRID:SCR_002285 | |
| Software, algorithm | FLIC analysis R code | Flidea | RRID:SCR_018386 | |
| Software, algorithm | DTrack | Other | S. Pletcher (University of Michigan) | |
| Other | FLIC Drosophila Feeding Monitors | Sable Systems | Model DFMV3 | https://www.sablesys.com/products/classic-line/flic_drosophila_behavior_system/ |
| Other | FLIR grasshopper camera | FLIR | GS3-U3-28S5C-C | #88-052 - GS3-U3-28S5C-C 2/3" FLIR Grasshopper3 High Performance USB 3.0 Color Camera |
| Other | Fujinon varifocal lens | Fujinon | MFR #DV3.4x3.8SA-1 | Fujinon 3 MP varifocal lens (3.8–13 mm, 3.4× zoom) |
