(A) Cartoon schematic of setup for filming flies on the Fly Liquid-Food Interaction Counter (FLIC). Frame capture is triggered by interactions of flies with the food source. (B) Frequency of all five main categories of observed feeding micro-behaviors: other (O), interaction (I), fast (F), long (L), long/quick (LQ). Each data point represents the total number of times an event occurred normalized to all observed events for each fly. N=8 flies, 528 events. (C) Percentage of flies from (B) that engaged in each feeding micro-behavior. (D) Representative temporal plots of sated (N=4 flies, 171 events) and starved (N=4 flies, 357 events) feeding micro-behaviors (left panel) and frequency of each behavior (right panel, two-tailed t-tests). Each row in left panel represents one fly, and frequencies are relative to all behaviors (two-tailed t-tests). (E) Heat maps of starved (left) and sated (right) transitional probabilities of observed behaviors from (D) (transitional probabilities are generated by dividing the observed count for each event pair by the total occurrences of the given event. p-Values are determined based on Z-scores, as described in Blumstein and Daniel, 2007). Long events contain both L and LQ events. (F) Frequency of each feeding micro-behavior during meal (7AM/PM-10AM/PM) vs. non-meal-times of day (one-tailed t-tests). N=9 flies, 693 events, frequency is relative to total interactions+feeding events. All error bars represent the mean +/- SEM.