(A) From simulation we can directly count the number of monomers in each quadrant, and generate the complete number ACF. We can also perform a stochastic localization experiment, to mimic experiment, producing excellent agreement. In each frame, a monomer was localized here with 60% probability, pact = 0.6. Reducing the probability increases the noisiness of the ACF, but not its amplitude or timescales. No other ‘error’ was introduced into the localization measurement. For all plots, the background signal is shown in dashed red. The background is the correlation of the total copies counted across the full surface (no separation into quadrants). It is 1 as expected for the simulations due to conservation of total copies when no measurement noise is introduced. (B) For the stochastic localization, we add blinking of the fluorophore. Each molecule, once localized, can be localized again within the 10 frames (1s) since its first localization, with maximal three total localizations. Each molecule is on average localized twice, based on the experimental characterization of the Dendra fluorophore (Saha and Saffarian, 2020). (C) Here, we have the probability of localizing a molecule decays with time, pact = 0.6exp(−t/10), such that early on, more localizations occur than later on in the trajectory. This mimics a distribution of activation times for the fluorophores. (D) Here, we set the surface of the sphere is assumed to be only partially ‘visible’ to the laser. Here, only the top 4 quadrants are detected and analyzed, and the bottom 4 quadrants are ‘dark’. Those monomers in the bottom half become visible once they diffuse into the top hemisphere. (E) Similar to (D), except here the visible part of the sphere is asymmetric. The lattice is shifted to a distance along the x-axis and z-axis and only the molecules whose distance to the origin is less than Rsphere = 67 nm are visible. Monomers outside of that region are ‘dark’, until they diffuse into the visible part of the surface.