Ventral striatum dopamine release encodes unique properties of visual stimuli in mice
- Cincinnati Children's Hospital Medical Center, United States
Abstract
The mesolimbic dopamine system is an evolutionarily conserved set of brain circuits that plays a role in attention, appetitive behavior, and reward processing. In this circuitry, ascending dopaminergic projections from the ventral midbrain innervate targets throughout the limbic forebrain, such as the ventral striatum/nucleus accumbens (NAc). Dopaminergic signaling in the NAc has been widely studied for its role in behavioral reinforcement, reward prediction error encoding, and motivational salience. Less well characterized is the role of dopaminergic neurotransmission in the response to surprising or alerting sensory events. To address this, we used the genetically encoded dopamine sensor dLight1 and fiber photometry to explore the ability of striatal dopamine release in to encode the properties of salient sensory stimuli in mice, such as threatening looming discs. Here, we report that lateral NAc (LNAc) dopamine release encodes the rate and magnitude of environmental luminance changes rather than visual stimulus threat level. This encoding is highly sensitive, as LNAc dopamine could be evoked by light intensities that were imperceptible to human experimenters. We also found that light-evoked dopamine responses are wavelength-dependent at low irradiances, independent of the circadian cycle, robust to previous exposure history, and involve multiple phototransduction pathways. Thus, we have further elaborated the mesolimbic dopamine system's ability to encode visual information in mice, which is likely relevant to a wide body of scientists employing light sources or optical methods in behavioral research involving rodents.
Data availability
Viral vector plasmids used in this study are available on Addgene. Codes used for fiber photometry signal extraction and analysis are available at https://www.tdt.com/docs/sdk/offline-data-analysis/offline-data-python/FibPhoEpocAveraging/. Codes used for visual stimulus generation are available at https://github.com/jelliottrobinson/Robinson_Lab. Source data is available in the provided Supplemental Data and Statistical Analysis file.
Article and author information
Author details
J Elliott Robinson
Funding
Simons Foundation Autism Research Initiative (BTI Award 663007)
- J Elliott Robinson
Gilbert Family Foundation (Team Science Award)
- J Elliott Robinson
Cincinnati Children's Research Foundation (Trustee Award)
- J Elliott Robinson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal husbandry and experimental procedures involving animal subjects were conducted in compliance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and approved by the Institutional Animal Care and Use Committee (IACUC) and by the Department of Veterinary Services at Cincinnati Children's Hospital Medical Center (CCHMC) under IACUC protocol 2020-0058.
Copyright
© 2023, Gonzalez et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Ventral striatum dopamine release encodes unique properties of visual stimuli in mice
eLife 12:e85064.
https://doi.org/10.7554/eLife.85064
