(A) Schematic of events in the two-alternative forced-choice (2AFC) task (L–R). (1) Rats self-initiated trials by breaking an IR beam in the central well. (2) After a variable length (200–700 ms) IR beam hold, one of two randomly selected cues are illuminated that signals which lever would be rewarded when pressed. (3) Cues maintained illumination until rats left the well, ‘Well Exit.’ The time from cue onset (2) to well exit (3) was identified as the decision time. (4) Animals had 5 s from cue onset to execute a decision via lever pressing, ‘Lever Press.’ The time from cue presentation (2) to lever press (4) was identified as the reaction time. (5) Possible trial outcomes were correct (sucrose reward given), incorrect, omission (no lever press within 5 s), or premature (well exit prior to cue presentation). After each trial, there was an intertrial interval where the house light was illuminated, and new trials could not be initiated (incorrect and omissions: 10 s; correct: 5 s). Created with BioRender.com. (B) Propranolol (green) decreased the number of trials initiated during a 2AFC task in females (p<0.0001, solid bars) and males (p=0.0066, open bars) compared to saline (blue). (C) Propranolol did not alter the percent of trials that animals left the well during the variable hold prior to cue presentation (premature) in females or males. (D) Propranolol decreased overall accuracy in females (p<0.001) but not males (p=0.3336). (E) The percent of trials that a response was omitted significantly increased only in females after propranolol (p<0.0001). (F) Altered task performance was not a result of decreased arousal – motor output or lever pressing ability. The ratio of lever presses made during the intertrial interval (ITI) to the total lever presses (lever press index) increased in both sexes by 150% after propranolol. (G) Decision time, or the latency to behaviorally discriminate cues and select an action, measured from cue onset to well exit, differed by sex and propranolol. Propranolol significantly increased the median decision times in females (p=0.0071) but not males (p=0.3086). (H) Behaviorally, median reaction times, from cue onset to lever press, were significantly altered by propranolol and sex. Propranolol significantly increased median reaction times in females (p=0.0101) but not males (p=0.4922). * Indicates a significant effect compared to saline *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. Behavioral data (n=19; female n=10, male n=9) is presented as mean ± SEM after confirming normality (Shapiro-Wilk). Comparisons between groups were made with REML ANOVA and Sidaks post hoc tests.