FAM76B regulates NF-κB-mediated inflammatory pathway by influencing the translocation of hnRNPA2B1

  1. Dongyang Wang
  2. Xiaojing Zheng
  3. Lihong Chai
  4. Junli Zhao
  5. Jiuling Zhu
  6. Yanqing Li
  7. Peiyan Yang
  8. Qinwen Mao  Is a corresponding author
  9. Haibin Xia  Is a corresponding author
  1. Shaanxi Normal University, China
  2. University of Utah, United States

Abstract

FAM76B has been reported to be a nuclear speckle localized protein with unknown function. In this study, FAM76B was first demonstrated to inhibit the NF-κB-mediated inflammatory pathway by affecting the translocation of hnRNPA2B1 in vitro. We further showed that FAM76B suppressed inflammation in vivo using a traumatic brain injury (TBI) mouse model. Lastly, FAM76B was shown to interact with hnRNPA2B1 in human tissues taken from patients with acute, organizing, and chronic TBI, and with different neurodegenerative diseases. The results suggested that FAM76B mediated neuroinflammation via influencing the translocation of hnRNPA2B1in vivo during TBI repair and neurodegenerative diseases. In summary, we for the first time demonstrated the role of FAM76B in regulating inflammation and further showed that FAM76B could regulate the NF-κB-mediated inflammatory pathway by affecting hnRNPA2B1 translocation, which provides new information for studying the mechanism of inflammation regulation.

Data availability

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX partner repository [1] with the dataset identifier PXD037539. ([1] Ma J, et al. (2019) iProX: an integrated proteome resource. Nucleic Acids Res, 47, D1211-D1217).

Article and author information

Author details

  1. Dongyang Wang

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  2. Xiaojing Zheng

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Lihong Chai

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  4. Junli Zhao

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Jiuling Zhu

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  6. Yanqing Li

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  7. Peiyan Yang

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    Competing interests
    The authors declare that no competing interests exist.
  8. Qinwen Mao

    Department of Pathology, University of Utah, Salt Lake, United States
    For correspondence
    Qinwen.Mao@path.utah.edu
    Competing interests
    The authors declare that no competing interests exist.
  9. Haibin Xia

    Department of Biochemistry, Shaanxi Normal University, Xi'an, China
    For correspondence
    hbxia2001@163.com
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2038-5759

Funding

The national natrual science foundation of China (81773265)

  • Haibin Xia

Key Research and Development Plan of Shaanxi Province (2018SF-106)

  • Haibin Xia

the natrual science foundation of Shaanxi Province (2023-JC-YB-642)

  • Xiaojing Zheng

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Brandon K Harvey, NIDA/NIH, United States

Ethics

Animal experimentation: All animal studies were performed in accordance with institutional guidelines and with approval by the Institutional Animal Care and Use Committee of Shaanxi Normal University (SNNU 2019-0128). Ethical permit of the use of the samples of human brain autopsy specimens was granted by the ethics committee of Shaanxi Normal University (SNNU 2019-0026).

Version history

  1. Received: December 19, 2022
  2. Preprint posted: December 30, 2022 (view preprint)
  3. Accepted: August 9, 2023
  4. Accepted Manuscript published: August 10, 2023 (version 1)
  5. Version of Record published: August 23, 2023 (version 2)

Copyright

© 2023, Wang et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Dongyang Wang
  2. Xiaojing Zheng
  3. Lihong Chai
  4. Junli Zhao
  5. Jiuling Zhu
  6. Yanqing Li
  7. Peiyan Yang
  8. Qinwen Mao
  9. Haibin Xia
(2023)
FAM76B regulates NF-κB-mediated inflammatory pathway by influencing the translocation of hnRNPA2B1
eLife 12:e85659.
https://doi.org/10.7554/eLife.85659

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https://doi.org/10.7554/eLife.85659