(A–B) Hippocampal vibratome sections from P35 wild-type and 4.1B KO mice immunostained for MBP as a myelin marker. Note the lack of myelin sheaths in the CA1 stratum radiatum of the hippocampus …
Length of myelinated axons and paranode density in the different layers of the CA1 hippocampus of wild-type and 4.1B KO mice.
CA1 hippocampus stained for MBP (green). (A, B, G, H) Lhx6-Cre;tdTomato mice: control (A, G), Cntn2-/- (B) and 4.1B KO (H) mice at P70 showing axons of PV and SST cells expressing the tdTomato …
Coronal brain sections showing the somato-sensory cortex of Lhx6-Cre;tdTomato control (A) and 4.1B KO (B) mice at P70. Double-staining for MBP (Cyan) and Caspr (green). Note in the inset that the …
Hippocampus from P25 wild-type and 4.1B KO mice immunostained for MBP (grey) and caspr (green). Note the severe loss of myelin sheaths in the CA1 stratum radiatum of the 4.1B-deficient hippocampus …
Hippocampus from P180 wild-type and 4.1B KO mice immunostained for MBP (grey). Note the severe loss of myelin sheaths in the CA1 stratum radiatum of the 4.1B-deficient hippocampus that persists at …
(A–D) Hippocampal sections of wild-type and 4.1B KO mice immunostained for PV (A, B) or SST (C, D) at P35. (E, F) Quantification of the cell density/105 µm2 in the CA1 stratum oriens (SO) and …
Density of PV and SST cells and length of myelinated PV and SST axons in the hippocampus of wild-type and 4.1B KO mice.
Hippocampal sections of wild-type and 4.1B KO mice at P35 immunostained for MBP (blue), SST (red) and Olig2 (green) as a marker of the oligodendrocyte lineage. CA1 region (A, B) and dentate gyrus (C,…
Density of Olig2-positive cells in the CA1 hippocampus and dentate gyrus of wild-type and 4.1B KO mice.
(A) Schematic drawing to depict the role of 4.1B at paranodes mediating the anchorage between Caspr and the ßII-spectrin at the boundary with the nodal ßIV-spectrin. (B) Distribution of internodal …
Internodal length of myelinated axons in the CA1 hippocampus of control and 4.1B KO mice.
Hippocampal sections of P70 Lhx6-Cre;tdTomato control and 4.1B KO mice were immunostained for MBP (blue), Caspr (white) and panNav (green). tdTomato expressed in Lhx6-positive cells or PV …
Length and positioning of Nav channels in GABAergic axons of control and 4.1B KO mice.
Hippocampal sections of P70 Lhx6-Cre;tdTomato control and 4.1B KO mice were immunostained for MBP (blue), Caspr (white) and panNav (green). tdTomato expressed in Lhx6-positive cells is in red. (A) a …
Immunostaining for Kv1.2 channels in GABAergic axons of the stratum radiatum in CA1 at P70. (A) Juxtaparanodal clustering of Kv1 channels in Lhx6-Cre;tdTomato control mouse immunostained for MBP …
Juxtaparanodal clustering of Kv1 channels in PV axons of wild-type, Cntn2-/-, Cntnap2-/-, and 4.1 KO mice.
(A–G) Patch-clamp recordings of Lhx6-positive PV fast-spiking interneurons in the stratum pyramidale (SP). (A) Schematic illustration of basket and bistratified (BiC) cells in CA1 located in the SP …
Electrophysiological properties of Lhx6-positive PV fast-spiking interneurons in the stratum pyramidale from control and 4.1B KO mice.
Electrophysiological properties of Lhx6-positive interneurons in the stratum oriens from control and 4.1B KO mice.
(A–C) Analysis of somatic action potentials (APs). Representative examples illustrating the voltage waveform of the AP (A1 and B1; in mV) from a control (A1) and a 4.1B KO neurons (B1). Below are …
Length and onset of AIS measured for AnkyrinG immunostaining of SST interneurons in the stratum oriens of wild-type and 4.1B KO mice.
(A) Schematic illustration of inhibitory inputs onto the soma, proximal and distal dendrites of pyramidal cells. (B) Example traces of sIPSCs onto CA1 pyramidal neurons of control and 4.1B KO mice. …
Amplitude and frequency of sIPSCs onto CA1 pyramidal neurons in the control and 4.1B KO mice.
Double-staining for MBP (pink) and SST (green). 25-z steps of 1 µm.
Double-staining for MBP (pink) and SST (green). 25-z steps of 1 µm.
Control (mean ± SEM, n=9) | 4.1B KO (mean ± SEM, n=10) | MW test, p value | |
---|---|---|---|
Passive properties | |||
Resting membrane potential (mV) | 57.67±3.26 | 60.50±1.99 | MW, 0.6748 |
Input resistance (MΩ) | 360.11±52.46 | 346.20±37.77 | MW, 0.8421 |
Rheobase (pA) | 36.67±5.00 | 28.80±6.33 | MW, 0.1305 |
Action potential parameters | |||
Amplitude (mV) | 60.42±3.47 | 56.54±2.79 | MW, 0.3882 |
Area (mV.s) | 58.69±6.00 | 56.44±4.74 | MW, 0.4967 |
Half-width (ms) | 0.92±0.07 | 0.94±0.06 | MW, 0.9838 |
Threshold (mV) | 46.79±2.28 | 46.60±1.78 | MW, 0.9682 |
AHP area (mV.ms) | 185.39±54.05 | 276.69±42.71 | MW, 0.2775 |
AHP peak (mV) | 10.35±1.85 | 10.65±1.10 | MW, 0.6607 |
Statistical differences between groups were calculated with a Mann-Whitney test (MW). Control: n=9 mice; 4.1B KO: n=6 mice.
Control (mean ± SEM, n=9) | 4.1B KO (mean ± SEM, n=17) | MW test, p value | |
---|---|---|---|
Passive properties | |||
Resting membrane potential (mV) | 62.17±2.93 | 57.59±1.65 | MW, 0.2527 |
Input resistance (MΩ) | 357.33±30.30 | 278.94±21.12 | MW, 0.0236* |
Rheobase (pA) | 38.25±5.73 | 52.65±5.79 | MW, 0.1221 |
Action potential parameters | |||
Amplitude (mV) | 65.34±1.84 | 64.06±1.94 | MW, 0.6788 |
Area (mV.s) | 111.44±17.87 | 104.80±8.93 | MW, 0.8788 |
Half-width (ms) | 1.61±0.25 | 1.63±0.16 | MW, 0.5929 |
Threshold (mV) | 45.69±1.83 | 43.65±1.43 | MW, 0.5558 |
AHP area (mV.ms) | 351.29±58.99 | 526.59±53.50 | MW, 0.0380* |
AHP peak (mV) | 12.57±1.79 | 16.24±1.25 | MW, 0.1280 |
Statistical differences between groups were calculated with a Mann-Whitney test (MW). Control: n=9 mice; 4.1B KO: n=8 mice.