(A) Immunostaining and quantification of TEAD1 +SCs on longitudinal sections of P22 WT and Tead1 cKO SNs. SC nuclei are identified by Sox10. All cell nuclei are marked by DAPI. n=3 mice per …
This zip archive contains source files for graphs in Figure 1A, B, C and D and uncropped labeled or unlabeled blots of Figure 1B and D.
TEAD2 expression is normalized to that of β-actin as an internal control. Arrow denotes ~65 kD TEAD2 band that was diminished with age.
This zip archive contains uncropped labeled or unlabeled blots in Figure 1—figure supplement 1.
(A) A living control (WT) and two Tead1 cKO mice from one litter at P55. Arrows point to splayed paralyzed hindlimbs. (B) Exposed SNs of WT and Tead1 cKO mice during dissection. Scale bar: 1 mm (C) …
This zip archive contains source files for graphs in Figure 2D, H, I, J, K and M.
(A) Representative images of semi-thin sections of various areas of WT and Tead1 cKO SNs at P22 and P50. Scale bar: 20 μm (B) Representative TEM images of transverse sections of WT and Tead1 cKO SNs …
(A) Immunostaining and quantification of SCs on transverse sections of WT and Tead1 cKO SNs at P3, P22, and P52. SC nuclei and all cell nuclei are marked by Sox10 and DAPI. Axons are marked by …
This zip archive contains source files for graphs in Figure 3A, B, C and D.
(A) Western blotting of P50 WT and Tead1 cKO SN lysates with anti-Oct6, -Krox20, -MPZ, and -MBP. Protein expression is normalized to that of β-actin as an internal control, and WT expression is …
This zip archive contains source files for graphs in Figure 4A, B, C and D and uncropped labeled or unlabeled blots of Figure 4A.
(A) Western blotting of P8 and P40 WT and Tead1 cKO SN lysates with anti-SREBP1, -SCD1, -SREBP2, -HMGCR, -FDPS, and -IDI1. Protein expression is normalized to that of β-actin as an internal control, …
This zip archive contains uncropped labeled or unlabeled blots of P8 WT and Tead1 cKO SNs in Figure 5A.
This zip archive contains source files for graphs in Figure 5A and B, and uncropped labeled or unlabeled blots of P40 WT and Tead1 cKO SNs in Figure 5A and P60 SNs of WT and YAP/TAZ cDKO in Figure 5B.
(A) Cartoon showing timeline of tamoxifen (TAM) injection and time of sacrifice of Tead1 iKO mice. (B) Representative TEM images of P150 WT and Tead1 iKO SNs showing occasional large axons …
This zip archive contains source files for graphs in Figure 6C and E.
This zip archive contains source files for graphs in Figure 6A–G and uncropped labeled or unlabeled blots of Figure 6A.
(A) Western blotting of adult WT and Tead1 iKO SN lysates with anti-TEAD1. TEAD1 expression is normalized to that of β-actin as an internal control, and WT expression is arbitrarily given the value …
(A) Immunostaining and quantification of Krox20 +SCs on longitudinal sections of WT and Yap1/Wwtr1 iDKO SNs 2 weeks after 1st tamoxifen injection. SC nuclei are marked by Sox10. All cell nuclei are …
This zip archive contains source files for graphs in Figure 7A–E and uncropped labeled or unlabeled blots of Figure 7D and E.
(A) Cartoon showing timeline of tamoxifen (TAM) injection and time of sacrifice of Tead1 iKO mice after nerve crush. (B) Low magnification images of longitudinal sections of ~4 mm long SN segments …
This zip archive contains source files for graphs in Figure 8E, F, G and H.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Mus musculus) | C57Bl/6 | Jackson Laboratory | Stock #: 000664; RRID:IMSR JAX:000664 | |
Genetic reagent (M. musculus) | Plp1-Cre-ERT2 | Leone et al., 2003 | MGI:2663093 | |
Antibody | anti-Yap/Taz (rabbit monoclonal) | Cell Signaling Technology | D24E4, #8418 RRID:AB_10950494 | IHC 1:200 Western 1:1000 |
Antibody | anti-SCG10 (rabbit monoclonal) | Novus Biologicals | NBP1-49461 RRID:AB_10011569 | IHC 1:5000 |
Antibody | anti-Yap (rabbit monoclonal) | Cell Signaling Technology | D8H1X, #14074 RRID:AB_2650491 | IHC 1:200 |
Antibody | anti-Sox10 (goat polyclonal) | R&D Systems | #AF-2864 RRID:AB_442208 | IHC 1:100 |
Antibody | anti-Sox10 (rabbit monoclonal) | Abcam | EPR4007, #ab155279 RRID:AB_2650603 | IHC 1:250 |
Antibody | anti-Egr2 (rabbit polyclonal) | Professor Dies Meijer, University of Edinburgh | IHC 1:4000 | |
Antibody | anti-Oct6 (rabbit monoclonal) | Abcam | EP5421, #ab126746 RRID:AB_11130256 | WB 1:1000 |
Antibody | Anti-Oct6 (rabbit polyclonal) | Abcam | #ab31766 RRID:AB_776899 | IHC 1:800 |
Antibody | Anti-c-Jun (mouse monoclonal) | BD Transduction Laboratories | #610326 RRID:AB_397716 | IHC 1:500 |
Antibody | Anti-c-Jun (rabbit monoclonal) | Cell Signaling Technology | 60 A8, #9165 RRID:AB_2130165 | WB 1:1000 |
Antibody | Anti-pS63-c-Jun (rabbit polyclonal) | Cell Signaling Technology | #9261 RRID:AB_2130162 | IHC 1:100 |
Antibody | anti-Ki67 (rabbit polyclonal) | Abcam | #ab15580 RRID:AB_443209 | IHC 1:200 |
Antibody | anti-p75NGFR (goat polyclonal) | Neuromics | #GT15057 RRID:AB_2737189 | IHC 1:400 |
Antibody | anti-Tubulin β3 (rabbit polyclonal) | Biolegend | #802001 RRID:AB_2564645 | IHC 1:1000 |
Antibody | IRDye-680 (goat anti-mouse) | LI-COR | #926–32220 RRID:AB_621840 | WB 1:15,000 |
Antibody | HRP-Goat anti-mouse secondary antibody | Jackson Immunoresearch | #715-035-150 RRID:AB_2340770 | WB 1:12,000 |
Antibody | HRP-Goat anti-rabbit secondary antibody | Jackson Immunoresearch | #115-055-062 RRID:AB_2338533 | WB 1:12,000 |
Chemical compound, drug | Araldite 6005 | EMS | #10920 | |
Chemical compound, drug | DDSA | EMS | #13710 | |
Chemical compound, drug | DBP | EMS | #13101 | |
Chemical compound, drug | BDMA | EMS | #11400–25 | |
Other | Coated grids (100 mesh) | EMS | #FF100-Cu | |
Chemical compound, drug | Osmium tetroxide (4% solution) | EMS | #19170 | |
Chemical compound, drug | Lead nitrate | EMS | #17900 | |
Chemical compound, drug | Sodium citrate | EMS | #21140 | |
Chemical compound, drug | Uranyl acetate | EMS | #22400 | |
Chemical compound, drug | Sodium borate | EMS | #21130 | |
Chemical compound, drug | Toluidine blue | EMS | #22050 | |
Chemical compound, drug | Paraformaldehyde | Sigma-Aldrich | #158127 | |
Commercial assay or kit | Click-It EdU Alexa Fluor 594 kit | ThermoFisher Scientific | #C10339 | |
Chemical compound, drug | EdU | ThermoFisher Scientific | #E10187 | |
Chemical compound, drug | Tamoxifen | Sigma-Aldrich | #T5648 | |
Other | DAPI stain | Invitrogen | #D1306 | IHC 1:250 |
Antibody | Alexa 488, 568 or 647 secondaries | Jackson Immunoresearch | IHC 1:250 to 1:1000 | |
Software, algorithm | Image Studio Lite | LI-COR, Inc | ||
Software, algorithm | Prism | GraphPad Software, Inc | ||
Software, algorithm | Stata | StataCorp LP | Mann-Whitney test |