Molecular mechanism of complement inhibition by the trypanosome receptor ISG65

  1. Alexander D Cook
  2. Mark Carrington  Is a corresponding author
  3. Matthew K Higgins  Is a corresponding author
  1. Department of Biochemistry, University of Oxford, United Kingdom
  2. Kavli Institute for Nanoscience Discovery, Dorothy Crowfoot Hodgkin Building, University of Oxford, United Kingdom
  3. Department of Biochemistry, University of Cambridge, United Kingdom
4 figures, 1 table and 5 additional files

Figures

Figure 1 with 1 supplement
Structure of the complex between ISG65 and human C3b.

(a) Composite volume of locally refined regions determined using cryogenic electron microscopy for ISG65 bound to human C3b. ISG65 is coloured in different shades of blue and green, as indicated in …

Figure 1—figure supplement 1
Workflow of Cryo-EM data processing.

Steps highlighted in blue were performed in SIMPLE, steps in green were performed in CryoSPARC, and steps in orange were performed in RELION.

Figure 2 with 1 supplement
ISG65 forms two distinct interfaces with the TED and CUB domains of C3b.

(a) The ISG65-C3b model shown in transparent cryo-EM density. The top panel shows the interface between ISG65 and the TED domain (orange), with bottom panel showing the interface between loop L2 of …

Figure 2—figure supplement 1
Surface plasmon resonance data.

Two experimental replicates of the data are shown in Figure 2c. Twofold serial dilutions from a concentration range of 5000–312.5 nM. Data shown here is derived from two biological replicates of …

ISG65 does not block the formation of the C3 convertase.

(a) The structure of the ISG65-C3b complex (without ISG65) docked into the electron microscopy-derived volumes obtained for the ISG65-C3b complex (left) and C3b alone (right). (b) Composite models …

Figure 4 with 1 supplement
ISG65 overlaps the binding sites for complement receptors 2 and 3.

Composite models obtained by docking the C3b-ISG65 structure onto those of C3b/d bound to factor H CCP19-20 (3OXU) (Morgan et al., 2011), CRIg (2ICF) (Wiesmann et al., 2006), CR1 CCP15-17 (5FO9) (For…

Figure 4—figure supplement 1
Comparison of ISG65 sequences from T. brucei brucei (green labels), T. brucei rhodesiense (blue labels), and T. brucei gambiense (red labels).

Tables

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Gene (Trypanosoma brucei brucei)ISG65G geneNCBI BioProject Accession: PRJEB46985UniProt: A0A8J9S0Z8
Gene (Homo sapiens)CFB geneNCBI GenBank accession: AF019413.1UniProt: P00751
Gene (H. sapiens)CFD geneNCBI GenBank accession: CH471139.2UniProt: P00746
Gene (H. sapiens)C3 geneNCBI GenBank accession:
AY513239.1
UniProt: P01024
Sequence-based reagentPrimersThis paperSee list of primers in the Appendeix. Primers were synthesised by Sigma.
Recombinant DNA reagentpHL-SEC vector backbonehttps://doi.org/10.1107/S0907444906029799; Aricescu et al., 2006
Cell line (H. sapiens)HEK293FGibcoR79007
Biological sample
(H. sapiens)
Human serumNHSBT non-clinical issue
SoftwareSIMPLE v3https://github.com/hael/SIMPLE/releases; Elmlund et al., 2020
SoftwareCryoSPARC v3https://cryosparc.com/; Structura Biotechnology Inc, 2020
SoftwareTOPAZ v0.2.4https://github.com/tbepler/topaz; Bepler and Noble, 2020
SoftwareRELION v3.1https://relion.readthedocs.io/en/release-3.1/index.html; RELION developer team, 2020
SoftwareDeepEMhancerhttps://github.com/rsanchezgarc/deepEMhancer; Sanchez Garcia, 2022
SoftwareAlphaFold2https://github.com/google-deepmind/alphafold; AlphaFold Team, 2021
SoftwareISOLDE v1.0https://github.com/tristanic/isolde; Croll, 2019
SoftwareCOOT v0.9.8.3https://github.com/pemsley/coot; Emsley, 2022
SoftwarePHENIX v1.20.1https://phenix-online.org; Phenix Development Group, 2022
SoftwareChimeraX v1.6https://www.cgl.ucsf.edu/chimerax/; UCSF Resource for Biocomputing, Visualization, and Informatics, 2023
SoftwareBIAevaluation v1.0Biacore, Cytiva, Marlborough, MA, USA
SoftwareFijihttps://imagej.net/software/fiji/

Additional files

Supplementary file 1

Supplementary Table 1: Cryo-EM data collection and model building statistics.

https://cdn.elifesciences.org/articles/88960/elife-88960-supp1-v1.docx
Supplementary file 2

Supplementary Table 2: Kinetic parameters of ISG65 variants binding to biotinylated C3b and C3d, as measured by surface plasmon resonance.

Average values and standard deviation from three experimental repeats are reported.

https://cdn.elifesciences.org/articles/88960/elife-88960-supp2-v1.docx
Supplementary file 3

Supplementary Table 3: Mass spectrometry analysis of ISG65-C3b conjugates observed in Figure 3.

The high molecular weight band observed in convertase assay in the presence of ISG65 was excised and analysed by mass spectrometry. The three most abundant proteins identified are presented.

https://cdn.elifesciences.org/articles/88960/elife-88960-supp3-v1.docx
Supplementary file 4

Primers.

https://cdn.elifesciences.org/articles/88960/elife-88960-supp4-v1.docx
MDAR checklist
https://cdn.elifesciences.org/articles/88960/elife-88960-mdarchecklist1-v1.docx

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