1. Microbiology and Infectious Disease

Parasite Communication: Learning the language of pathogens

Parasites can use extracellular vesicles and cellular projections called cytonemes to communicate with one another.
https://doi.org/10.7554/eLife.89264
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  1. Izadora Volpato Rossi
  2. Marcel Ivan Ramirez
(2023)
Parasite Communication: Learning the language of pathogens
eLife 12:e89264.
https://doi.org/10.7554/eLife.89264
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  1. Izadora Volpato Rossi
  2. Marcel Ivan Ramirez  Is a corresponding author
  1. Graduate Program in Cell and Molecular Biology, Federal University of Paraná, Brazil
  2. Carlos Chagas Institute, FIOCRUZ, Brazil

To survive and replicate, pathogens must evade the immune system of the organisms they infect by adapting to the environment inside them. The individual pathogens that manage to do this will survive and multiply, and particularly successful adaptations may lead to the emergence of new strains of the pathogen (Bashey, 2015; Mideo, 2009). Understanding how this happens – including how pathogens communicate with each other, and how they respond to the microbiota of their host (Kalia et al., 2020) – is crucial to efforts to combat the diseases caused by parasites and other pathogens.

Decades of studying host-pathogen interactions has revealed several mechanisms of pathogen virulence, such as variation of surface antigens to avoid immune detection and the release of molecules that neutralize the host immune system and promote pathogen invasion (Casadevall and Pirofski, 2001). Specific structures and organelles that pathogens employ – such as flagella to allow swimming – have also been discovered (Khan and Scholey, 2018).

Cells have been shown to communicate with one another by exchanging membrane-bound ‘extracellular vesicles’ – which contain proteins, lipids and genetic material – with neighbouring cells (van Niel et al., 2018; Raposo and Stahl, 2019). Cells can also communicate via plasma membrane protrusions called ‘filopodia’ which contain bundles of actin filaments and have roles in cellular adherence and migration (Roy and Kornberg, 2015). There are multiple types of filopodia, categorized by their size and origin.

Now, in eLife, Natalia de Miguel (Instituto Tecnológico Chascomús and Escuela de Bio y Nanotecnologías) and colleagues – including Nehuén Salas and Manuela Blasco Pedreros as joint first authors – report that different strains of the protozoan Trichomonas vaginalis, a parasite responsible for sexually-transmitted infections, communicate using extracellular vesicles and a type of filopodia called a cytoneme (Salas et al., 2023). Cytonemes are thin specialized filopodia that can traffic signalling proteins (Roy and Kornberg, 2015).

T. vaginalis is a single-celled parasite which colonizes the human urogenital tract and adheres to epithelial cells. Strains that are highly adherent can form clumps more easily than other strains when cultured, and it has been shown that these strains are more cytotoxic to host cells (Coceres et al., 2015; Lustig et al., 2013). Through live imaging, Salas et al. showed that cytonemes are visible on the surface of T. vaginalis, and that highly adherent strains have more cytonemes than poorly adherent strains. The number of parasites displaying cytonemes also increased when clumps formed, with the individual parasites within the clumps being connected by cytonemes.

In elegant experiments using inserts with a porous membrane that prevents direct contact between parasites – but allows secreted factors through – Salas et al. demonstrate that extracellular vesicles of less adherent strains stimulate the growth of cytonemes in the most adherent strain. Moreover, overexpressing the protein VPS32 – which regulates the biogenesis of small vesicles – stimulated the induction of cytonemes, suggesting their formation is, in part, contact-independent.

These findings raised the question of what signals within extracellular vesicles from poorly adherent strains stimulate cytoneme formation in highly adherent strains. To investigate, Salas et al. compared the proteins expressed in the extracellular vesicles of multiple strains of T. vaginalis, finding differences in protein expression but conservation of the biological processes they are involved in. The strains shared essential components of metabolic processes, signal response, development and locomotion. Furthermore, all strains contained proteins associated with the formation of cytonemes.

Finally, Salas et al. harnessed the ability of T. vaginalis to change from a swimming flagellate form to an amoeboid when it adheres to prostate cells, in order to investigate how communication impacts its behavior during infection (Kusdian et al., 2013). Again, through controlled experiments using porous inserts, they showed that less adherent strains induce the amoeboid morphology and the adhesion of the highly adherent strain to prostate cells. Surprisingly, this parasite-to-parasite communication also doubled the adhesion of a poorly adherent strain to the cells.

The experiments provide solid evidence of the participation of extracellular vesicles in communications between parasites, as well as the presence of specific membranous structures that allow this communication. The finding that parasites of different strains communicate with one another raises fundamental questions related to parasitism and the pathology of Trichomonas. Why do poorly adherent strains have a greater effect on the formation of cytonemes by adherent strains than their own strain? Do the secreted extracellular vesicles signal the presence of another strain, alerting nearby parasites to enhance their adherence in order to outcompete competitors? Future work should also investigate the role of microbiota and infections that often occur alongside Trichomonas, such as Mycoplasma (Margarita et al., 2020), in parasite communication and behavior.

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Does parasite communication result in competition or cooperation?

Different strains of Trichomonas vaginalis (yellow and green) communicate with one another through the release of extracellular vesicles and the formation of membrane protrusions called cytonemes (depicted in inset). This communication can lead to increased adherence of the parasites to epithelial cells. It is not clear whether this communication leads to competition (left), where one strain (yellow) enhances its adherence in order to outcompete a less adherent strain (green), or whether it leads to cooperation (right) where a less adherent strain (green) becomes more adherent after contact with a highly adherent strain (yellow).

References

    1. Bashey F
    (2015) Within-host competitive interactions as a mechanism for the maintenance of parasite diversity
    Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 370:20140301.
    https://doi.org/10.1098/rstb.2014.0301

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Author details

  1. Izadora Volpato Rossi

    Izadora Volpato Rossi is in the Graduate Program in Cell and Molecular Biology, Federal University of Paraná, Curitiba, Brazil
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5495-8167

  2. Marcel Ivan Ramirez

    Marcel Ivan Ramirez is at the Carlos Chagas Institute, FIOCRUZ, Curitiba, Brazil
    For correspondence
    marcel.ivan.ramirez@gmail.com
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6917-1954

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© 2023, Rossi and Ramirez

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Izadora Volpato Rossi
  2. Marcel Ivan Ramirez
(2023)
Parasite Communication: Learning the language of pathogens
eLife 12:e89264.
https://doi.org/10.7554/eLife.89264

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    Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication

    Nehuén Salas, Manuela Blasco Pedreros ... Natalia de Miguel
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    Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication

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    Trichomonas vaginalis, the etiologic agent of the most common non-viral sexually transmitted infection worldwide. With an estimated annual prevalence of 276 million new cases, mixed infections with different parasite strains are expected. Although it is known that parasites interact with their host to enhance their own survival and transmission, evidence of mixed infections call into question the extent to which unicellular parasites communicate with each other. Here, we demonstrated that different T. vaginalis strains can communicate through the formation of cytoneme-like membranous cell connections. We showed that cytonemes formation of an adherent parasite strain (CDC1132) is affected in the presence of a different strain (G3 or B7RC2). Our findings provide evidence that this effect is contact-independent and that extracellular vesicles (EVs) are responsible, at least in part, of the communication among strains. We found that EVs isolated from G3, B7RC2, and CDC1132 strains contain a highly distinct repertoire of proteins, some of them involved in signaling and communication, among other functions. Finally, we showed that parasite adherence to host cells is affected by communication between strains as binding of adherent T. vaginalis CDC1132 strain to prostate cells is significantly higher in the presence of G3 or B7RC2 strains. We also observed that a poorly adherent parasite strain (G3) adheres more strongly to prostate cells in the presence of an adherent strain. The study of signaling, sensing, and cell communication in parasitic organisms will enhance our understanding of the basic biological characteristics of parasites, which may have important consequences in pathogenesis.

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