Optogenetic stimulation of single ganglion cells in the living primate fovea
- The Institute of Optics, University of Rochester, United States
- Center for Visual Science, University of Rochester, United States
- Flaum Eye Institute, University of Rochester, United States
Figures
Figure 1
Cells chosen for stimulation.
The cell circled in red marks the targeted cell. The averaged fluorescence traces for each cell show the integrated intensity over the cell’s soma. The red marks on the time axis represent the onset of the 800 ms optogenetic stimulus. The histograms show each cell’s response in terms of z score. (a) Results from male macaque. (b) Results from female macaque. All targeted cells are at similar eccentricities (1.5°).
Figure 2
ΔF/F for each cell soma plotted against that cell’s distance from the targeted cell.
Note that even the nearby (<50 µm) cell somas do not show a significantly elevated response (p>>0.05, unpaired t-test) than other cells at more distant locations. The leftmost point on each plot, colored in red, corresponds to the targeted cell itself. The traces for the four closest neighbors are shown below each scatter plot.
Figure 3
Relevant absorption/emission spectra for our fluorophores and optogenetic actuator.
The emission wavelengths of tdTomato and GCaMP6 are well suited to activating ChrimsonR, and the wavelength used to image GCaMP fluorescence stimulates both fluorophores well.
Additional files
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Optogenetic stimulation of single ganglion cells in the living primate fovea
eLife 12:RP90050.
https://doi.org/10.7554/eLife.90050.3
