RIM-BP2 regulates Ca2+ channel abundance and neurotransmitter release at hippocampal mossy fiber terminals

Version of Record

The authors declare this version of their article to be the Version of Record.

Download
Cite
Share
CommentOpen annotations (there are currently 0 annotations on this page).

Version of Record published: February 8, 2024 (This version)
Reviewed preprint version 2: January 19, 2024 (Go to version)
Reviewed preprint version 1: November 9, 2023 (Go to version)
Preprint posted: July 19, 2023 (Go to version)
Sent for peer review: July 18, 2023

1. Of interest
A new ‘CFS tracking’ paradigm reveals uniform suppression depth regardless of target complexity or salience

David Alais, Jacob Coorey ... Matthew J Davidson

Research Article Apr 29, 2024
Further reading

Abstract
eLife assessment
Introduction
Results
Discussion
Materials and methods
Data availability
References
Article and author information
Metrics

Abstract

Synaptic vesicles dock and fuse at the presynaptic active zone (AZ), the specialized site for transmitter release. AZ proteins play multiple roles such as recruitment of Ca²⁺ channels as well as synaptic vesicle docking, priming, and fusion. However, the precise role of each AZ protein type remains unknown. In order to dissect the role of RIM-BP2 at mammalian cortical synapses having low release probability, we applied direct electrophysiological recording and super-resolution imaging to hippocampal mossy fiber terminals of RIM-BP2 knockout (KO) mice. By using direct presynaptic recording, we found the reduced Ca²⁺ currents. The measurements of excitatory postsynaptic currents (EPSCs) and presynaptic capacitance suggested that the initial release probability was lowered because of the reduced Ca²⁺ influx and impaired fusion competence in RIM-BP2 KO. Nevertheless, larger Ca²⁺ influx restored release partially. Consistent with presynaptic recording, STED microscopy suggested less abundance of P/Q-type Ca²⁺ channels at AZs deficient in RIM-BP2. Our results suggest that the RIM-BP2 regulates both Ca²⁺ channel abundance and transmitter release at mossy fiber synapses.

eLife assessment

Miyano et al. study the impact of RIM-BP2 deletion at mossy fiber synapses using direct electrophysiological recordings from mossy terminals and STED super-resolution microscopy. The article addresses an important question in the field of synaptic transmission and provides compelling evidence demonstrating reduced calcium channel abundance in mossy terminals upon RIM-BP2 removal.

https://doi.org/10.7554/eLife.90799.3.sa0

About eLife assessments

Introduction

At chemical synapses, an action potential arriving at the presynaptic terminal opens voltage-gated Ca²⁺ channels, and Ca²⁺ influx through voltage-gated Ca²⁺ channels triggers neurotransmitter release from synaptic vesicles within milliseconds. Synaptic vesicle fusion occurs at a specialized region of the presynaptic membrane called the active zone (AZ), where Ca²⁺ channel clusters and vesicle fusion sites are accommodated via AZ scaffold proteins (Südhof, 2012). For rapid transmission, accumulation of Ca²⁺ channels, co-localization of Ca²⁺ channels and fusion sites, and molecular priming of synaptic vesicles for fusion are required, and the differences among these factors might provide a basis for synaptic diversity. However, it remains unknown how these factors are regulated by AZ-scaffold proteins.

Rab3-interacting molecule-binding proteins (RIM-BPs) represent one principal, conserved family of AZ proteins and bind to RIMs and Ca²⁺ channels (Wang et al., 2000; Hibino et al., 2002). RIM-BP family includes RIM-BP1, RIM-BP2, and RIM-BP3 in mammals, but RIM-BP3 expression is low in the nervous system (Mittelstaedt and Schoch, 2007). In Drosophila neuromuscular junctions (NMJs), loss of RIM-BPs decreases Ca²⁺ channel density and reduces release probability (Liu et al., 2011). Moreover, RIM-BP in Drosophila NMJs is necessary for tight coupling of synaptic vesicles to Ca²⁺ channels and replenishment of high release probability vesicles (Liu et al., 2011; Müller et al., 2015; Petzoldt et al., 2020). In mammalian synapses, the observations from KO mice are diverse. In RIM-BP1,2 DKO mice, the coupling between Ca²⁺ channels and synaptic vesicles becomes loose, and action potential-evoked neurotransmitter release is reduced at the calyx of Held synapse (Acuna et al., 2015; Butola et al., 2021). At hippocampal CA3-CA1 synapses, RIM-BP2 deletion alters Ca²⁺ channel localization at the AZs without altering total Ca²⁺ influx. Besides, RIM-BP1,2 DKO has no additional effect, indicating that RIM-BP2 dominates the function of RIM-BP isoforms (Grauel et al., 2016; see also Krinner et al., 2021). Tight coupling between Ca²⁺ channels and vesicles is crucial for rapid and efficient transmitter release (Wadel et al., 2007).

In contrast, hippocampal mossy fiber-CA3 synapses are characterized by low release probability (Vyleta and Jonas, 2014). At hippocampal mossy fiber synapses, gSTED imaging suggests that RIM-BP2 KO alters the Munc13-1 cluster number and distribution. In addition, RIM-BP2 deletion decreases the number of docked vesicles (Brockmann et al., 2019). However, because of technical difficulty, direct and quantitative measurements of exocytosis in RIM-BP2 KO terminals have not been performed so far.

To quantify the RIM-BP2 function in mossy fiber-CA3 synapses, we performed whole-cell patch-clamp recordings from WT and RIM-BP2 KO hippocampal mossy fiber boutons. From electrophysiological recordings, we found that the reductions of Ca²⁺ current amplitudes in RIM-BP2 KO terminals. The EPSC and capacitance measurements suggested that the reduction of Ca²⁺ currents was responsible, in part, for that of transmitter release. In addition, they suggested that fusion competence was impaired, which could be overcome by larger Ca²⁺ influx into the terminal. Using STED imaging, we found the abundance of P/Q-type Ca²⁺ channels in terminals to be reduced in RIM-BP2 KO. We suggest that RIM-BP2 regulates the number of P/Q-type Ca²⁺ channels at the AZ and is critical for transmitter release at hippocampal mossy fiber terminals.

Results

RIM-BP2 KO reduces presynaptic calcium currents at hippocampal mossy fiber boutons

To investigate the roles of RIM-BP2 in synaptic transmission, we examined the kinetics of exocytosis and Ca²⁺ influx in WT and RIM-BP2 KO synapses using presynaptic whole-cell capacitance measurements (Figure 1). When the terminal was depolarized from –80 mV to +10 mV for 10 ms, Ca²⁺ currents and membrane capacitance were recorded as shown in Figure 1A. During the depolarizing pulse, capacitance was not measured due to large conductance changes, and the increase was used for measuring synaptic vesicle exocytosis. To determine the size of the RRP and the time course of exocytosis, we measured capacitance changes (ΔC_m) in response to various durations of depolarization. Here, the length of the depolarizing pulse was varied between 5 ms and 100 ms, and ΔC_m was plotted against pulse duration (Figure 1B, Figure 1—figure supplement 1, bottom). ΔC_m became larger as the duration of pulse was prolonged, but the increase started to be saturated at a 100 ms pulse in WT terminals, suggesting depletion of the RRP. ΔC_m in response to a 100 ms pulse was 52 ± 8.1 fF (n = 9, Figure 1B). The amplitude corresponds to the fusion of about 500–600 synaptic vesicles (Hallermann et al., 2003; Midorikawa and Sakaba, 2017). The time course of ΔC_m could be fitted by a single exponential with a time constant of 59 ± 12 ms. In RIM-BP2 KO terminals, ΔC_m in response to a 100 ms pulse was somewhat smaller (41 ± 5.9 fF, n = 8) than that of WT terminals, and the release time constant was somewhat slower (64 ± 14 ms) than in WT terminals. We should note that the time constant is not necessarily reliable because the ΔC_m values do not reach a plateau value at 100 ms pulse in some terminals. Although the capacitance jumps of KO may be smaller for shorter pulses, capacitance measurements are not sensitive enough to detect small changes (<5 fF, 50 vesicles, see Figures 5 and 6). Nevertheless, the differences in the time course of capacitance were not significantly different between WT and KO (p=0.23 from ANOVA) and the effects on release examined by strong pulses depleting the RRP were relatively minor. The amplitudes of Ca²⁺ currents were smaller in RIM-BP2 KO terminals compared to WT terminals (Figure 1B, Figure 1—figure supplement 1, top, p<0.02 from ANOVA). The Ca²⁺ current in response to a 100 ms pulse was reduced by ~30% in RIM-BP2 KO terminals (27 ± 3.9 pA, n = 8) compared to WT terminals (44 ± 5.4 pA, n = 9) (p=0.0248, t-test, Figure 1B). Thus, the results show that RIM-BP2 deletion reduces presynaptic Ca²⁺ influx, but effects on the RRP size and the time course of release measured by capacitance measurements are relatively minor.

Figure 1 with 1 supplement see all

Download asset Open asset

Presynaptic calcium currents and synaptic vesicle release in RIM-BP2 KO mice.

(A) The terminal was depolarized from –80 mV to +10 mV in WT (black) and RIM-BP2 KO (gray) hippocampal mossy fiber boutons. Ca²⁺ currents (I_Ca) and membrane capacitance (C_m) in response to a 10 ms pulse (V_m) are shown. (B) Top: the peak Ca²⁺ currents are plotted against the pulse duration. WT vs KO, p=0.034 (ANOVA). Bottom: the capacitance jumps are plotted against the pulse duration. WT vs KO, p=0.23 (ANOVA). Black filled circles and gray open circles represent the data from WT (n = 9 from eight animals) and RIM-BP2 KO (n = 8 from six animals), respectively. Each data point represents mean ± SEM. (C) Experimental protocol and representative traces for presynaptic Ca²⁺ current measurements in WT (black) and RIM-BP2 KO (gray) boutons. Terminals were sequentially depolarized for 5 ms with 2 s intervals from –80 mV to +70 mV by 10 mV steps. (D) Current–voltage relationships of peak Ca²⁺ currents in WT (black filled circle; n = 4–6 from four animals) and RIM-BP2 KO (gray open circle; n = 4–5 from five animals). I_Cas were elicited by 5 ms depolarizations. Two curves were significantly different (p<0.01 from mixed model). Each data point represents mean ± SEM. Numerical values of plots are provided in Figure 1—source data 1.

Figure 1—source data 1 Datasets of Ca²⁺ current and capacitance amplitudes presented in Figure 1 and Figure 1—figure supplement 1.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig1-data1-v1.xlsx
Download elife-90799-fig1-data1-v1.xlsx

To investigate the Ca²⁺ influx in more detail, we examined the voltage dependence of Ca²⁺ currents. Figure 1C shows voltage step protocols and representative current traces. In both genotypes, Ca²⁺ currents started to be activated at around –20 mV, and maximal amplitude was observed at ~+10 mV (Figure 1D). There was no major difference in the voltage dependence of Ca²⁺ currents between WT and RIM-BP2 KO mice. Consistently, activation time constants of Ca²⁺ currents were similar between WT and RIM-BP2 KO when the terminal was depolarized to +10 mV (τ = 1.4 ± 0.2 ms in WT, n = 6; τ = 1.3 ± 0.2 ms in KO, n = 4). These results suggest a reduction in the number of Ca²⁺ channels rather than changes in activation kinetics is responsible for the current decrease observed.

The effects of extracellular calcium concentration on Ca²⁺ influx and capacitance changes in WT and RIM-BP2 KO

Because Ca²⁺ currents were reduced in RIM-BP2 KO mice, we tested whether the reduction of Ca²⁺ currents could be restored by elevation of the external Ca²⁺ concentration (Thanawala and Regehr, 2013). We raised the extracellular Ca²⁺ concentration ([Ca²⁺]_ext) from 2 mM to 4 mM (Figure 2). Ca²⁺ currents and membrane capacitances were recorded at 4 mM [Ca²⁺]_ext, and amplitudes then plotted against pulse duration (Figure 2A, Figure 2—figure supplement 1). Although Ca²⁺ current elicited by a 100 ms pulse in RIM-BP2 KO was still smaller (33 ± 2.7 pA, n = 3) than that in WT (54 ± 6.1 pA, n = 6) (Figure 2B, top) in 4 mM Ca²⁺, Ca²⁺ currents of KO mice at 4 mM [Ca²⁺]_ext were more comparable to those of WT at 2 mM [Ca²⁺]_ext.

Figure 2 with 1 supplement see all

Download asset Open asset

The effects of high extracellular calcium concentration on Ca²⁺ currents and capacitance changes.

(A) The I_Cas (top) and the C_ms (bottom) are plotted against the pulse duration. Black filled squares and gray open squares represent the data from WT (n = 6–8 from six animals) and RIM-BP2 KO (n = 3–4 from two animals) at 4 mM [Ca²⁺]_ext, respectively. For comparison, the WT data in 2 mM [Ca²⁺]_ext are superimposed (black filled circle; n = 9) (the same datasets as Figure 1B). Each data point represents mean ± SEM. I_Cas were significantly different between WT and KO in 4 mM [Ca²⁺]_ext (p<0.01 from mixed model). The WT I_Cas in 2 mM [Ca²⁺]_ext and the KO I_Cas in 4 mM [Ca²⁺]_ext were significantly different (p<0.02 from mixed model), but C_m were not significantly different (p=0.57 from mixed model). (B) Average I_Cas (top) and C_ms (bottom) in response to a 100 ms pulse in WT (black bars) and RIM-BP2 KO (gray bars) terminals. Extracellular Ca²⁺ concentration was 2 mM (left) or 4 mM (right). Error bars show SEM. Circles indicate individual values. Numerical values of plots are provided in Figure 2—source data 1.

Figure 2—source data 1 Datasets of Ca²⁺ current and capacitance amplitudes presented in Figure 2 and Figure 2—figure supplement 1.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig2-data1-v1.xlsx
Download elife-90799-fig2-data1-v1.xlsx

At 4 mM [Ca²⁺]_ext, ΔC_m in response to a 100 ms pulse in RIM-BP2 KO was 51 ± 11 fF (n = 3) and showed a similar value in WT at 2 mM [Ca²⁺]_ext (Figure 2B, bottom). At the same time, ΔC_m in WT was not altered (52 ± 2.5 fF, n = 6) at higher [Ca²⁺]_ext. The time course of exocytosis at 4 mM [Ca²⁺]_ext in KO was similar to that of WT at 2 mM [Ca²⁺]_ext as the time courses were superimposable in Figure 2A (p=0.57 from mixed model).

Next, we adjusted Ca²⁺ current amplitudes of WT to that of RIM-BP2 KO by lowering [Ca²⁺]_ext (Figure 3). The amplitudes of Ca²⁺ currents and capacitance jumps were plotted against pulse duration (Figure 3A, Figure 3—figure supplement 1). At 1 mM [Ca²⁺]_ext, Ca²⁺ currents and ΔC_m were smaller than those of RIM-BP2 KO at 2 mM [Ca²⁺]_ext. At 1.5 mM [Ca²⁺]_ext, Ca²⁺ currents became more comparable to those of RIM-BP2 KO at 2 mM [Ca²⁺]_ext (p=0.12 from mixed model). Here, the ΔC_m evoked by a 100 ms pulse (43 ± 6.7 fF, n = 5) was comparable between WT and RIM-BP2 KO (Figure 3A). The average time course of capacitance increase of WT at 1.5 mM [Ca²⁺]_ext was similar to that of RIM-BP2 KO at 2 mM [Ca²⁺]_ext (p=0.88 from mixed model). Therefore, by reducing Ca²⁺ currents, WT data could reproduce similar release time course of RIM-BP2 KO. In Figure 3B, ΔC_m in response to a 100 ms pulse at various [Ca²⁺]_exts were plotted against the peak Ca²⁺ current amplitudes. The relationship could be fitted by a Hill plot with n = 3 in WT (Schneggenburger et al., 1999). Consistently, when capacitance jumps elicited by various pulse durations are plotted against total Ca²⁺ influx (Figure 3—figure supplement 2), the RIM-BP2 KO data were superimposed on the WT data. In Figure 3C, relative amplitudes of Ca²⁺ currents and capacitance in response to a 100 ms pulse are plotted. The RIM-BP2 KO data were superimposed on the WT data, suggesting that the RRP size was similar between WT and KO (~50 fF). Taken together, presynaptic capacitance measurements were not able to detect differences in the release rates between WT and KO especially when Ca²⁺ influx in WT and KO was adjusted to be similar. In particular, the RRP sizes measured by the pool depleting stimulation were not altered strongly by RIM-BP2 KO. The results may mean that RIM-BP2 is dispensable for transmitter release or that large Ca²⁺ or large Ca²⁺ influx may overcome the deficit of the release. Alternatively, capacitance measurements may not be sensitive enough to detect the differences between WT and KO at short stimulation, which are relevant for AP-evoked release (see Figures 5 and 6).

Figure 3 with 2 supplements see all

Download asset Open asset

Calcium dependence of the release kinetics and the RRP size.

(A) Top: the relationship between peak Ca²⁺ currents and pulse durations at different [Ca²⁺]_exts. Bottom: the relationship between capacitance jumps and pulse durations at different [Ca²⁺]_exts. Gray open circles, black diamonds, and black triangles represent the data from RIM-BP2 KO at 2 mM [Ca²⁺]_ext (n = 8) (the same datasets as Figure 1B), WT at 1 mM [Ca²⁺]_ext (n = 3–5 from four animals) and WT at 1.5 mM [Ca²⁺]_ext (n = 5–7 from six animals), respectively. Each data point represents mean ± SEM. (B) Capacitance jumps at various [Ca²⁺]_exts are plotted against calcium current amplitudes. Pulses were 100 ms depolarization from –80 mV to +10 mV. Each data point represents mean ± SEM. Data points were fitted with a Hill equation with n = 3. (C) Left: average I_Cas at indicated [Ca²⁺]_exts were normalized to the response at 2 mM [Ca²⁺]_ext in each genotype. Right: average C_ms at indicated [Ca²⁺]_exts were normalized to the 2 mM [Ca²⁺]_ext response in each genotype. Numerical values of plots are provided in Figure 3—source data 1.

Figure 3—source data 1 Datasets of numerical values presented in Figure 3 and Figure 3—figure supplement 1.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig3-data1-v1.xlsx
Download elife-90799-fig3-data1-v1.xlsx

High EGTA experiments suggest unaltered coupling between calcium channels and synaptic vesicles

Previous studies have used Ca²⁺ chelator such as ethylene glycol tetraacetic acid (EGTA) to examine the sensitivity of release to intracellular Ca²⁺ buffers (Adler et al., 1991; Borst and Sakmann, 1996; Vyleta and Jonas, 2014). When the physical coupling between Ca²⁺ channels and synaptic vesicles is tight, EGTA (slow Ca²⁺ chelator) has less effect on release probability, but EGTA is effective when the coupling is loose. Previous experiments have suggested that RIM-BPs regulated the channel–vesicle coupling at the calyx of Held (Acuna et al., 2015). If this observation also applies to the mossy fiber synapse, EGTA sensitivity of release should be higher in RIM-BP2 KO terminals. To investigate the coupling at mossy fiber boutons in RIM-BP2 KO, we changed the concentration of EGTA in the patch pipette. In the presence of 5 mM EGTA, we depolarized the terminal from –80 mV to +10 mV for 5 ms and recorded Ca²⁺ currents and membrane capacitance (Figure 4A). Ca²⁺ currents and ΔC_m were plotted against pulse duration (Figure 4B, Figure 4—figure supplement 1). In both WT and RIM-BP2 KO mice, ΔC_m were reduced under 5 mM EGTA compared with the control condition of 0.5 mM EGTA. We compared the effect of EGTA on average amplitudes of peak Ca²⁺ currents in response to a 20 ms pulse (Figure 4D). Interestingly, Ca²⁺ current amplitudes were ~1.2 times larger at 5 mM EGTA (53 ± 12 pA, n = 5) than at 0.5 mM EGTA (43 ± 5.1 pA, n = 9) in WT, though the data scattered under 5 mM EGTA condition and there was no statistically significant difference (p=0.36, t-test). In RIM-BP2 KO, we did not observe such an increase. EGTA might inhibit Ca²⁺ channel inactivation in WT (von Gersdorff and Matthews, 1996). In Figure 4C, we compared the effect of EGTA on the ΔC_m in response to a 20 ms pulse. In both genotypes, ΔC_m values were inhibited by 5 mM EGTA, and the inhibition was somewhat larger in RIM-BP2 KO (EGTA inhibition: p<0.01, genotypes p>0.05 from mixed model).

Figure 4 with 1 supplement see all

Download asset Open asset

The effects of high EGTA on calcium currents and synaptic vesicle exocytosis.

(A) Example traces in response to a 5 ms depolarizing pulse to +10 mV in WT (left) and RIM-BP2 KO (right) boutons. Ca²⁺ current (I_Ca) and membrane capacitance (C_m) recorded with 0.5 mM EGTA (black) or 5 mM EGTA (red) in the patch pipette are shown. Note that each trace was obtained from different terminals but the traces were superimposed. (B) The I_Cas (top) and C_ms (bottom) are plotted against the pulse duration. The patch pipette contained 5 mM EGTA. Black filled diamonds and gray open diamonds represent the data from WT (n = 4–5 from four animals) and RIM-BP2 KO (n = 4 from three animals), respectively. Each data point indicates mean ± SEM. (**C, D**) Average C_ms (C) and I_Cas (D) elicited by a 20 ms pulse in WT (filled bars) and RIM-BP2 KO (hatched bars). The extracellular Ca²⁺ concentration was 1 mM (from five animals) or 2 mM. The intracellular solution contained either 0.5 mM EGTA (black) or 5 mM EGTA (red). Error bars show SEM. Circles and diamonds indicate individual values. Numerical values of plots are provided in Figure 4—source data 1.

Figure 4—source data 1 Datasets of Ca²⁺ current and capacitance amplitudes presented in Figure 4 and Figure 4—figure supplement 1.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig4-data1-v1.xlsx
Download elife-90799-fig4-data1-v1.xlsx

Ca²⁺ current amplitudes in RIM-B2 KO are smaller than in WT (Figure 4B). It is possible that the strong effect of high EGTA on release may be due to reduced Ca²⁺ currents in RIM-BP2 KO. Therefore, we changed [Ca²⁺]_ext from 2 mM to 1 mM and adjusted Ca²⁺ current amplitudes of WT to the RIM-BP2 KO level. The capacitance increase and the amplitudes of Ca²⁺ currents became similar to those of RIM-BP2 KO (Figure 4C and D). These results indicated that WT and RIM-BP2 KO terminals had similar sensitivity of neurotransmitter release to EGTA. Thus, it is unlikely that changes in the coupling distance are responsible for the reduced exocytosis of the mutants.

Smaller AP-evoked EPSCs in KO could not be explained entirely by the reduced Ca²⁺ influx

While Ca²⁺ current amplitudes were clearly reduced in KO, capacitance jumps were reduced little. Capacitance measurements could only detect large amounts of exocytosis (~5 fF, 50 vesicles), and the changes relevant for the AP-evoked release (<10 vesicles per AP per synapse) could not be detected. Moreover, large Ca²⁺ influx used for the stimulation protocol of Figures 1–3 could overcome the deficits of RIM-BP2 KO. In order to detect the changes related to physiological conditions, we measured the AMPA-EPSCs evoked by electrical mossy fiber stimulation at CA3 pyramidal cells (Figure 5). As seen in Figure 5A and B , the EPSCs were evoked by dual stimulation of mossy fibers. The mossy fiber-evoked responses were confirmed by the relatively faster EPSC kinetics and sensitivity to DCG-IV (1 μM), an mGluR2 agonist (remaining response of <30%, 0.23 ± 0.01 for WT [n = 5], 0.29 ± 0.02 for KO [n = 5]). Because multiple fibers were stimulated, we could not estimate the number of vesicles released per one synapse. Nevertheless, when fibers were stimulated similarly, the EPSC amplitudes of KO were much smaller (893 ± 202 pA in WT, n = 7; 189 ± 73 pA in KO, n = 7) (p=0.011). In Figure 2, presynaptic recordings indicated that Ca²⁺ current amplitudes of KO at 4 mM [Ca²⁺]_ext became similar to those of WT at 2 mM [Ca²⁺]_ext (Figure 2). The EPSCs became larger when the [Ca²⁺]_ext was raised from 2 mM to 4 mM in KO (Figure 5A, top, see also Figure 6—figure supplement 1) and became more similar to those of WT. In Figure 3, presynaptic recording showed that I_Cas of WT at 1.5 mM [Ca²⁺]_ext were similar to those of KO at 2 mM [Ca²⁺]_ext. However, the WT EPSCs at 1.5 mM [Ca²⁺]_ext seemed larger than KO at 2 mM [Ca²⁺]_ext (Figure 5A, bottom, see also Figure 6—figure supplement 1). Changing the [Ca²⁺]_ext from 1.5 mM to 2 mM in WT and from 2 mM to 4 mM in KO should have the same potentiation effect on the EPSCs if the reduced Ca²⁺ current amplitudes are the only mechanism for reduced EPSCs in KO. In Figure 5B, we calculated two types of potentiation ratios: in WT, the ratio of the EPSC response in 2 mM to that in 1.5 mM [Ca²⁺]_ext was calculated in each cell. In KO, the ratio of the EPSC response in 4 mM to that in 2 mM [Ca²⁺]_ext was calculated in each cell. When these two values were compared, the ratios were much larger in KO (Figure 5B, p<0.01, t-test). Pr is more sensitive to the [Ca²⁺]_ext in the low end of the dose–response curve because of the third to fourth power dependence of release on [Ca²⁺]_ext at the low end (Dodge and Rahamimoff, 1967; Schneggenburger and Neher, 2000; Bollmann et al., 2000). Therefore, the result suggests lower release probability in KO even when the amounts of Ca²⁺ influx were adjusted to be similar. When the paired pulse ratios were compared, all four conditions (WT in 1.5 mM and 2 mM [Ca²⁺]_ext, KO in 2 mM and 4 mM [Ca²⁺]_ext) provided similar values (Figure 5C). The paired pulse ratio should be inversely correlated to Pr, in principle. However, paired pulse facilitation in mossy fiber synapses could be mediated by multiple effects, including Ca²⁺-independent broadening of AP waveforms (Geiger and Jonas, 2000) and Ca²⁺ buffer saturation, which is more pronounced and increases the paired-pulse ratios at higher [Ca²⁺]_ext (Blatow et al., 2003). Therefore, the value might not be sensitive to the [Ca²⁺]_ext.

Figure 5

Download asset Open asset

The evoked EPSC amplitudes in WT and KO, and their sensitivity to the extracellular Ca^2+.

(A) The mossy fiber-evoked EPSCs measured at CA3 pyramidal cells. The fibers were stimulated twice with an interval of 20 ms. The responses of WT in 1.5 mM and 2 mM [Ca²⁺]_ext (n = 7 cells from five animals), as well as those of KO in 2 mM and 4 mM [Ca²⁺]_ext (n = 7 cells from six animals) are shown. The stimulus artifacts were blanked. (B) The amplitude ratios of WT EPSCs (2 mM/1.5 mM [Ca²⁺]_ext) and KO EPSCs (4 mM/2 mM [Ca²⁺]_ext) are shown. The concentrations were chosen to set the Ca²⁺ influx similar between WT and KO from Figures 1—3. (C) The paired pulse ratios under four conditions (WT in 1.5 mM and 2 mM [Ca²⁺]_ext, KO in 2 mM and 4 mM [Ca²⁺]_ext). Numerical values of plots are provided in Figure 5—source data 1.

Figure 5—source data 1 Datasets of numerical values presented in Figure 5.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig5-data1-v1.xlsx
Download elife-90799-fig5-data1-v1.xlsx

We also analyzed the EPSCs in response to a stimulus train (50 Hz × 25 or 26 pulses) both in WT and KO (Figure 6) under different external Ca²⁺ concentrations. When the WT and KO responses were compared, the time course of facilitation/depression was quite different between WT and KO (Figure 6A–C). In 2 mM [Ca²⁺]_ext, WT responses showed initial facilitation (approximately twofold) followed by depression, whereas KO responses showed pronounced facilitation of approximately tenfold. The difference could be due to both reduced Ca²⁺ currents and impaired fusion competence in KO.

Figure 6 with 2 supplements see all

Download asset Open asset

The time course of synaptic facilitation/depression in WT and KO.

(A) Mossy fibers were stimulated at 50 Hz (25 times) and the evoked EPSCs were measured in 1.5 mM (n = 5 cells from four animals) and 2 mM [Ca²⁺]_ext (n = 7 cells from five animals) in WT. The stimulus artifacts were blanked. (B) Mossy fibers were stimulated at 50 Hz (26 times in this particular example), and the evoked EPSCs were measured in 2 mM (n = 7 cells from six animals) and 4 mM [Ca²⁺]_ext (n = 8 cells from six animals). (C) The time course of EPSC amplitudes during a 50 Hz train. The data were obtained from WT and KO in 2 mM [Ca²⁺]_ext. The time courses were significantly different (p<0.01 from ANOVA). (D) The same as (C), but the data were obtained from WT in 1.5 mM [Ca²⁺]_ext and KO in 2 mM [Ca²⁺]_ext. The time courses were significantly different (p<0.05 from ANOVA). (E) The same as (C), but the data were obtained from WT in 2 mM [Ca²⁺]_ext and KO in 4 mM [Ca²⁺]_ext. Numerical values of plots are provided in Figure 6—source data 1. The time courses were not significantly different (p=0.12 from ANOVA). Numerical values of plots are provided in Figure 6—source data 1.

Figure 6—source data 1 Datasets of numerical values presented in Figure 6 and Figure 6—figure supplements 1 and 2.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig6-data1-v1.xlsx
Download elife-90799-fig6-data1-v1.xlsx

To adjust presynaptic Ca²⁺ influx to the similar level as the KO terminals, the [Ca²⁺]_ext was reduced to 1.5 mM in WT. Under this condition, presynaptic Ca²⁺ current amplitudes were expected to be similar between WT and KO (in 2 mM [Ca²⁺]_ext). If the reduced Ca²⁺ influx is only responsible for smaller EPSCs in KO, we will observe similar time course of train responses between WT and KO. However, facilitation was much less in the WT condition (at 1.5 mM [Ca²⁺]_ext, Figure 6D, p<0.05 from ANOVA), suggesting the mechanism independent of Ca²⁺ influx. In order to obtain the same Ca²⁺ influx as that of WT in 2 mM [Ca²⁺]_ext, the [Ca²⁺]_ext was increased to 4 mM in KO. The time course of facilitation was somewhat larger in the KO condition compared with that WT in 2 mM [Ca²⁺]_ext. Because of the data scatter in KO, the time course was statistically not significant (p>0.05 from ANOVA). Because multiple fibers were stimulated, the absolute EPSC amplitudes depend on not only the release probability and the number of release sites per synapse, but also the number of stimulated synapses. Nevertheless, when the absolute amplitudes were plotted against the stimulus number, the differences were most prominent at the first responses in the train and the differences were diminished later in the train, suggesting that the initial release probability was likely to be lower at RIM-BP2 KO synapses, but the release could be restored during repetitive stimulation (Figure 6—figure supplement 1). Restoration of release is consistent with minor difference of capacitance changes in response to the depolarization (Figures 1—4).

STED microscopy is consistent with less abundance of P/Q-type Ca²⁺ channels in RIM-BP2 KO

Our electrophysiological data suggest that RIM-BP2 may regulate the abundance of Ca²⁺ channels. In order to directly study the Ca²⁺ channel abundance at the AZ, we performed STED microscopy analysis of Ca²⁺ channels in WT and RIM-BP2 KO hippocampal mossy fiber terminals. We here focused on P/Q-type and N-type Ca²⁺ channels because both Ca²⁺ channel types are relevant for transmitter release at this synapse (Castillo et al., 1994; Pelkey et al., 2006; Li et al., 2007). We performed immunohistochemistry on thin brain cryosections from WT and RIM-BP2 KO mice. STED microscopy confirmed complete loss of RIM-BP2 proteins in KO terminals (Figure 7—figure supplement 1). We then immunohistochemically labeled the α-subunit of either P/Q-type Ca²⁺ channel (Cav2.1) or N-type Ca²⁺ channel (Cav2.2) and Munc13-1 (AZ/release site marker, Böhme et al., 2016; Sakamoto et al., 2018) with VGLUT1 (presynaptic marker) and PSD-95 (postsynaptic marker) (Figure 7A and B, Figure 7—figure supplement 2). In the stratum lucidum of the hippocampal CA3 region, mossy fibers form large synapses containing multiple and clustered AZs on CA3 pyramidal cells, and also small en passant and filopodial synapses containing a single AZ on interneurons (Acsády et al., 1998; Rollenhagen et al., 2007). Consistent with this, large VGLUT1-positive terminals rarely contacted interneurons marked with two major interneuron markers, mGluR1α and GluA4 (Figure 7—figure supplement 3). Thus, we identified AZs using Munc13-1 STED images and quantified the signal intensity of Cav2.1 or Cav2.2 only at AZs clustered in large VGLUT1-positive terminals in the CA3 stratum lucidum, allowing us to confine the analysis to large mossy fiber terminals innervating onto CA3 pyramidal cells. The total signal intensity of Cav2.1 at the AZ was 22% lower in RIM-BP2 KO mice (n = 4 animals) than in WT mice (n = 4 animals) (p=0.0286; Figure 7C, left). These data are consistent with the reduction of Ca²⁺ currents in RIM-BP2 KO mice (Figure 1), though STED microscopy is nonlinear and can only provide qualitative differences regarding intensity. In addition, the spatial resolution of STED microscopy (especially z axis) does not assign the signals to the large mossy fiber terminal rigidly. The area, length, and width of Cav2.1 clusters in the AZ were not different between WT and RIM-BP2 KO terminals (Figure 7—figure supplement 4). The analysis in this study might not have spatial resolution to detect the differences. The analysis from the confocal microscopy also indicated that the signal intensity of Cav2.1 was reduced in KO, consistent with the STED data (Figure 7—figure supplement 5).

Figure 7 with 5 supplements see all

Download asset Open asset

RIM-BP2 deletion alters the signal intensity of Cav2.1 clusters within the active zone (AZ).

(A) Confocal (top) and STED (bottom) images of Munc13-1 (magenta) and Cav2.1 (yellow) clusters at hippocampal mossy fiber terminals of WT (left) and RIM-BP2 KO (right) mice. Scale bar: 500 nm. (B) Confocal images of PSD95 (cyan) and VGLUT1 (white) to identify glutamatergic synapses in CA3 stratum lucidum: mossy fiber-CA3 synapses. Scale bar: 500 nm. The image was taken from same region shown in (A, left). (C) Histograms (top) and cumulative distribution plots (bottom) of the total signal intensity of Cav2.1 (left), Cav2.2, and Munc13-1 (right) at AZs in WT (black) and RIM-BP2 KO (red) mice. (D) The average nearest-neighbor distance between Cav2.1 and Munc13-1 clusters in WT (black; n = 4 animals) and RIM-BP2 KO (red; n = 4 animals) mice. Several hundreds of AZs per image were analyzed. Data show the average value of distance per animal, and error bars represent SEM. Each data point indicates individual values. Numerical values of plots are provided in Figure 7—source data 1.

Figure 7—source code 1 Custom-designed programs in Mathematica.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig7-code1-v1.zip
Download elife-90799-fig7-code1-v1.zip
Figure 7—source data 1 Datasets of numerical values presented in Figure 7.: https://cdn.elifesciences.org/articles/90799/elife-90799-fig7-data1-v1.xlsx
Download elife-90799-fig7-data1-v1.xlsx

In contrast to Cav2.1, the total signal intensity of Cav2.2 and Munc13-1 did not significantly differ between WT and RIM-BP2 KOs (p=0.3143 and 0.0831) (Figure 7C, right), though it remains possible that we failed to detect some changes. Furthermore, we found that the number of Cav2.1 clusters within the AZ identified by STED deconvolution analysis was not altered (2.5 ± 0.11 in WT, n = 4 animals; 2.3 ± 0.02 in KO, n = 4 animals) (p=0.114). These data are consistent with the idea that RIM-BP2 KO reduces the abundance of P/Q-type Ca²⁺ channels per cluster. To optically estimate physical distances between Ca²⁺ channels and release sites, we next analyzed the nearest-neighboring distance between Cav2.1 and Munc13-1 clusters. These distances were unchanged (61 ± 1.4 nm in WT, n = 4 animals; 64 ± 1.3 nm in KO, n = 4 animals) (p=0.200) (Figure 7D), consistent with the similar sensitivity of release to EGTA between WT and RIM-BP2 KO mice. However, the resolution of STED microscopy cannot allow detection of small differences of the cluster number and the distance between WT and KO. From these results together with presynaptic recordings, we suggest that decreased Ca²⁺ influx in RIM-BP2 KO hippocampal mossy fiber terminals is caused by less abundance of P/Q-type Ca²⁺ channels at the AZ.

Discussion

Accumulation of Ca²⁺ channels at the AZ, and efficient vesicle docking and priming are essential factors for fast synaptic vesicle exocytosis. AZ proteins are critical for fast synchronous release and synaptic diversity. RIM-BPs have been implicated in recruitment of Ca²⁺ channels to the AZ (Kaeser et al., 2011; Liu et al., 2011) by interacting with RIMs and Ca²⁺ channels (Wang et al., 2000; Hibino et al., 2002). In addition, RIM-BPs, like RIM1/2, recruit Munc-13 and accelerate synaptic vesicle priming (Brockmann et al., 2020). At murine central synapses, RIM-BPs-deficient synapses demonstrate overall a rather mild decrease in the amounts of transmitter release (Acuna et al., 2015; Grauel et al., 2016; Luo et al., 2017; Krinner et al., 2017; Brockmann et al., 2019; Butola et al., 2021). Most studies so far have been mainly focused on synapses with high release probability (phasic synapses) such as the calyx of Held and CA3-CA1 synapses. That said, at mossy fiber-CA3 synapses, KO of RIM-BPs has a strong influence on synaptic transmission (Brockmann et al., 2019). Notably, at mossy fiber-CA3 synapses, release probability is relatively low (tonic synapses). It was unknown whether the reduction of transmitter release at RIM-BP2 KO mossy fiber synapses was due to impaired Ca²⁺ channel recruitment and/or impaired vesicle docking or priming. Using STED microscopy, Brockmann et al., 2019 suggested that the physical recruitment of Munc13-1 might be an important function of RIM-BP2, though functional demonstration had been lacking.

By using direct presynaptic patch-clamp recordings, we here observed a decrease of Ca²⁺ current amplitudes (~30%) in RIM-BP2 KO mice (Figure 1). Consistently, STED microscopy supported reduced abundance of P/Q-type Ca²⁺ channels (Cav2.1) in the mutant mossy fiber terminal (Figure 7). Interestingly, this observation is similar to that at Drosophila NMJ and hair cell synapses (Liu et al., 2011; Krinner et al., 2017), but not that at other synapses (Acuna et al., 2015; Grauel et al., 2016; Butola et al., 2021), suggesting that the functional role of RIM-BP2 in recruiting Ca²⁺ channels differs among synapse types.

Brockmann et al., 2019 observed a reduction of Munc13-1 cluster number and docked vesicles in RIM-BP2-deficient synapses at hippocampal mossy fiber terminals (Figure 7). They identified the terminals by using ZnT3, enriched at mossy fiber (Brockmann et al., 2019; Wenzel et al., 1997). Our STED imaging did not detect differences in Munc13-1 cluster number at the AZ between WT and RIM-BP2 KO mice. Notably, we analyzed the number of Cav2.1, Cav2.2, and Munc13-1 clusters only within AZs showing direct co-localization with VGLUT1 and PSD-95, which mainly reflects the AZs facing CA3 pyramidal cells rather than the synapses onto interneurons (Acsády et al., 1998; Rollenhagen et al., 2007). Such a difference in areas analyzed between previous and our study might explain the difference. In addition, some developmental variability/compensation could still occur in the range we studied and be relevant for the discrepancy between Munc13-1 results between Brockmann et al., 2019 and this study. Also, the spatial resolution of our study is limited and we cannot rigidly measure the signals only from large mossy fiber terminals. It is important to state that our results do not exclude roles of RIM-BP2 in synaptic vesicle priming. Indeed, we have shown that the reduction of the EPSC amplitudes could not be fully explained by the reduced Ca²⁺ influx (Figure 5). Because the differences in the RRP size measured from capacitance measurements were relatively minor (Figures 1—3), the fusion competence was likely to be impaired by RIM-BP2 KO, but this could be overcome by large Ca²⁺ influx. Consistently, the EPSCs responses of KO became larger and comparable to those of WT (Figure 6A and B, Figure 6—figure supplement 1) during repetitive AP stimulation, at least qualitatively.

Recent studies have reported that RIM-BPs deletion altered Ca²⁺ channel localization and loosened the coupling between Ca²⁺ channels and synaptic vesicles at mammalian synapses (Acuna et al., 2015; Grauel et al., 2016; Butola et al., 2021). We investigated the Ca²⁺ channel–vesicle coupling at hippocampal mossy fiber terminals. The sensitivity of release to Ca²⁺ chelator EGTA was not changed between WT and RIM-BP2 KO mice. We also indicated RIM-BP2 KO did not alter the coupling of Ca²⁺ channels to release sites by using STED microscopy, though spatial resolution of STED microscopy in this study could not allow to detect small changes (Figure 7).

At some mammalian synapses, deletion of RIM-BP2 affects physical distance between Ca²⁺ channels and synaptic vesicles (Acuna et al., 2015; Grauel et al., 2016; Butola et al., 2021). In contrast, at hippocampal mossy fiber terminals having low release probability (Vyleta and Jonas, 2014), RIM-BP2 KO decreased the abundance of P/Q-type Ca²⁺ channels, thereby reducing Ca²⁺ influx and neurotransmitter release. We hypothesize the specific molecular–architectural and biochemical contributions by RIM-BPs might be of different relevance among different types of synapses. Brockmann et al., 2020 proposed that RIM-BP2 controlled Ca²⁺ channel recruitment and vesicle priming by profound interaction with RIMs and Munc13s. These results have been obtained by studying hippocampal cultures dominated by phasic synapses. It seems possible that the interaction among three protein types is tight at some types of phasic synapses, simply because of the high density of these proteins at AZs. Alternatively, one might speculate about additional proteins, different interaction surfaces taken, and transsynaptic columns (Tang et al., 2016). Deletion of RIM-BP2 does not lead to loss of Ca²⁺ channels themselves and/or vesicle priming due to remaining RIMs, Munc13, and other proteins, which can regulate recruitment of Ca²⁺ channels and synaptic vesicle priming without RIM-BP2. Instead, fine-tuning of release such as synchronization is compromised. We hypothesize that AZ scaffold might be differently built, leading to different consequences when eliminating one component. Hence deletion of RIM-BP2 leads to immediate loss of Ca²⁺ channels and vesicle priming. This is in line with the proposal that at hippocampal mossy fiber synapses, synaptic vesicles are only loosely coupled with Ca²⁺ channels (Vyleta and Jonas, 2014) and vesicles are de-primed under resting conditions (Miki et al., 2016; Neher and Brose, 2018). Future research along this line may lead to an understanding of how fine molecular differences in the AZ scaffolds might orchestrate synaptic diversity.

Although this study reveals reduction of Ca²⁺ currents and reduced fusion competence in RIM-BP2 KO mossy fiber synapses, it has some limitation. Most importantly, with conventional knockouts the observed changes could reflect compensatory developmental changes and not direct outcomes of the RIM-BP2 knockout. This issue should be resolved by using region-specific and time-dependent conditional KO.

Materials and methods

Slice preparation

Request a detailed protocol

All animal experiments were conducted in accordance with the guidelines of the Physiological Society of Japan and were approved by Doshisha University Animal Experiment Committee (A22063, D22063).

We used male and female C57BL/6 mice (postnatal days 35–40). WT and RIM-BP2 (encoded by the Rimbp2 locus) KO mice (Grauel et al., 2016) were deeply anesthetized with isoflurane and decapitated. Their brains were quickly removed and chilled in sherbet-like cutting solution containing (in mM) 87 NaCl, 75 sucrose, 25 NaHCO₃, 1.25 NaH₂PO₄, 2.5 KCl, 10 glucose, 0.5 CaCl₂, and 7 MgCl₂ bubbled with 95% O₂ and 5% CO₂ (Hallermann et al., 2003). Hippocampal slices (300 μm thick) were prepared from brains using a vibratome (VT1200S, Leica) in ice-cold cutting solution. After slicing, slices were incubated in cutting solution at 37°C for 30 min and subsequently kept at room temperature (22–25°C) up to 4 h.

Whole-cell recordings

Request a detailed protocol

Electrophysiological recordings were performed in a recording chamber filled with the extracellular solution containing (in mM) 125 NaCl, 2.5 KCl, 25 glucose, 25 NaHCO₃, 1.25 NaH₂PO₄, 0.4 ascorbic acid, 3 myo-inositol, 2 Na-pyruvate, 2 CaCl₂, and 1 MgCl₂ saturated with 95% O₂ and 5% CO₂. In some experiments, the concentration of CaCl₂ was changed to 1 mM, 1.5 mM, and 4 mM. For presynaptic membrane capacitance measurements, 1 μM tetrodotoxin (TTX, Wako) was added to block Na⁺ channels. Moreover, for recording Ca²⁺ currents (Figure 1B), 10 mM TEA-Cl was added to block K⁺ channels. Slices were visualized by an upright microscope (BX-51, Olympus). Whole-cell patch-clamp recordings were applied to hippocampal mossy fiber terminals and CA3 pyramidal cells at room temperature. The patch pipettes were filled with the intracellular solution containing (in mM) 135 Cs-gluconate, 20 TEA-Cl, 10 HEPES, 5 Na₂-phosphocreatine, 4 MgATP, 0.3 GTP, and 0.5 EGTA (pH = 7.2 adjusted with CsOH). In some experiments, the concentration of EGTA was changed to 5 mM. Presynaptic patch pipettes (BF150-86-10, Sutter Instrument) had a resistance of 15–20 MΩ and series resistance (R_s) was 30–70 MΩ. R_s was compensated so that residual resistance was 30–35 MΩ. For postsynaptic recordings, the same internal solution was used, except that EGTA concentration was raised to 5 mM. Postsynaptic patch pipettes (BF150-86-10, Sutter Instrument) had a resistance of 3–7 MΩ and series resistance (R_s) was 5–20 MΩ, which was compensated by 50–80%.

Patch-clamp recordings were performed using an EPC10/3 or EPC10/2 amplifier (HEKA) in voltage-clamp mode, controlled by Patchmaster software (HEKA). Membrane currents were low-pass filtered at 2.9 kHz and sampled at 20 or 50 kHz.

Membrane capacitance measurements were performed using an EPC10/3 amplifier in the sine + DC configuration (Lindau and Neher, 1988; Gillis, 2000) using Patchmaster software (HEKA). A sine wave (30 mV in amplitude, 1000 Hz in frequency) was superimposed on the holding potential of –80 mV. The terminal was depolarized from –80 mV to +10 mV for 5–100 ms. The IV of presynaptic Ca currents were measured by sequential depolarized for 5 ms, with 2 s intervals from –80 mV to +70 mV by 10 mV steps.

For the EPSC measurements, postsynaptic cells (CA3 pyramidal cells) were voltage clamped at –60 to –80 mV. For Figures 5 and 6, the data were corrected for the different holding potential (re-calculated to the value at –70 mV assuming the reversal potential of 0 mV, which may have the correction effect of ~15%). At the end of the experiments, DCG-IV was applied to confirm mossy fiber synaptic responses, whenever possible. The mossy fibers were stimulated extracellularly using a glass electrode used for patch clamp containing the extracellular solution and the stimulation intensity was usually 10–60 V.

Data were obtained from at least three different WT and RIM-BP2 KO terminals in each set of experiments (biological replicate).

Immunostaining

Request a detailed protocol

Brains of WT and RIM-BP2 KO mice were quickly removed and transferred to cryomold (Sakura Finetek) filled with tissue freezing medium (Leica). Brains were instantaneously frozen on aluminum block in liquid nitrogen and stored at –80°C. For cryosectioning, frozen brains were kept in cryostat (CM1860, Leica) at –18°C for 30 min. Then, 10-μm-thick sections were sliced and collected on cover glasses (Matsunami glass, 25 × 25 No.1). Sections were quickly fixed by dehydration with a heat blower at 50°C for 1 min and further dehydrated in ethanol for 30 min at –25°C and in acetone for 10 min on ice. After blocking with PBS containing 0.3% BSA at room temperature, sections were incubated with primary antibodies diluted in PBS containing 0.3% BSA: anti-VGLUT1 (1:2000, Synaptic Systems, guinea pig polyclonal, RRID:AB_887878), anti-PSD-95 (1:50, NeuroMab, mouse monoclonal IgG2a, clone K28/43, TC Supernatant, RRID:AB_2877189), anti-Munc13-1 (1:1000, mouse monoclonal IgG1, clone 11B-10G, Sakamoto et al., 2018), and anti-Cav2.1 (1:400, Synaptic Systems, rabbit polyclonal, RRID:AB_2619841) or anti-Cav2.2 (1:400, Synaptic Systems, mouse monoclonal IgG2b, clone 163E3, RRID:AB_2619843) or anti-RIM-BP2 (1:200, mouse monoclonal IgG2b, clone 8-4G, Sakamoto et al., 2022) for 3 hr at room temperature. Afterward, sections were washed and incubated with fluorescence dye-labeled secondary antibodies: DyLight 405 Anti-Guinea Pig IgG (Jackson ImmunoResearch, RRID:AB_2340470), Alexa Fluor 488 (Thermo Fisher Scientific)-labeled Anti-Mouse IgG2a (Jackson ImmunoResearch, RRID:AB_2338462), STAR635P (Abberior)-labeled Anti-Mouse IgG1 (Jackson ImmunoResearch, RRID:AB_2338461), and Alexa Fluor 594 (Thermo Fisher Scientific)-labeled Anti-Rabbit IgG (Jackson ImmunoResearch, RRID:AB_2340585) or Anti-Mouse IgG2b (Jackson ImmunoResearch, RRID:AB_2338463) for 1 hr at room temperature. Sections were post-fixed with 4% PFA in PBS and washed with PBS. Sections were mounted on coverslips using Prolong Glass Antifade Mountant (Thermo Fisher Scientific). The same number of sections were obtained from WT and RIM-BP2 KO brains and processed in parallel at the same experimental day (biological replicate).

For Figure 7—figure supplement 3, only WT brains were used. Primary antibodies used were anti-VGLUT1 (1:2000, Synaptic Systems, rabbit polyclonal, RRID:AB_887877), anti-VGLUT1 (1:2000, Synaptic Systems, guinea pig polyclonal, RRID:AB_887878), anti-mGluR1α (1:200, Nittobo Medical, guinea pig polyclonal, mGluR1a-GP-Af660), anti-GluA4C (1:100, Nittobo Medical, rabbit polyclonal, GluR4C-Rb-Af160), and anti-Munc13-1 (1:1000, mouse monoclonal IgG1, clone 11B-10G).

STED imaging

Request a detailed protocol

STED imaging was performed using TCS SP8 STED 3× microscope (Leica) equipped with a 405 nm diode laser, a pulsed white light laser (WLL), a continuous 592 nm STED laser for alignment, a pulsed 775 nm STED laser, HyD detectors, and a 100× oil-immersion objective lens (NA = 1.4). STED images were acquired using the Leica LAS-X software with an image format of 1024 × 1024 pixels, 16-bit sampling, 8-line accumulations, and 11.36-zoom factor, yielding a pixel size of 10 nm. HyD detectors were configured to counting mode with a gating from 0.5 to 6.5 ns. A 405 nm diode laser was used to excite DyLight405. Alexa488, Alexa594, and STAR635P were excited using WLL at 488 nm, 561 nm, and 633 nm, respectively. The 775 nm STED laser power was set to 75 and 100% of maximum power for depletion of STAR635P and Alexa594, respectively, and delay time was set to 300 ps. STED imaging was performed on thin sections obtained from four different WT and RIM-BP2 KO mice (biological replicate). Images were acquired three times from each section (technical replicate).

Analysis

For electrophysiological experiments, the data were analyzed using Igor Pro (WaveMetrics) or Excel (Microsoft Corp). Presynaptic calcium currents were measured at the peak amplitude during the depolarizing pulse. Capacitance jumps were measured between baseline (before the pulse) and ~10 ms after the pulse where the effect of tail currents became minor. To determine the time constant of release, an exponential fit was applied. Values are given as mean ± SEM, and n indicates the number of recorded terminals or the number of animals used. Statistical method of sample size determination was not done, but our sample sizes are similar to those of previous studies (Hallermann et al., 2003; Midorikawa and Sakaba, 2017).

For STED imaging, data were analyzed using custom-designed programs in Mathematica (Wolfram), values were processed using Igor Pro (WaveMetrics), and pictures were created with ImageJ (NIH). To determine the area of AZs, image masks were generated from STED images of Munc13-1 by unsharp masking and image binarization. Only AZs that show co-localization with VGLUT1 and PSD-95 immunofluorescence signals were included in the analysis. To confine the analysis to the large mossy fiber boutons, AZs in presynaptic terminals of small VGLUT1-positive area were excluded. Then, the background-subtracted integral of signal intensity of Cav2.1 or Cav2.2 was quantified using the image masks of AZs. The background signal intensity was estimated from non-AZ area in the same STED images. For quantification of Cav2.1 clusters in the AZs, STED images of Cav2.1 were deconvolved using a Gaussian kernel with a radius of 40 nm. Image masks of each Ca²⁺ channel were generated from deconvolved STED images by unsharp masking and image binarization. Then, the total signal intensity, the size, and the number of Cav2.1 clusters at the AZs were quantified using the image masks of Cav2.1 clusters. Custom-designed programs in Mathematica (Wolfram) are available as Figure 7—source code 1. Values are given as mean ± SEM, and n indicates the number of animals. Statistical method of sample size determination was not done, but our sample sizes are similar to those of previous studies (Brockmann et al., 2019).

Experiments were not fully randomized and blinded.

Statistical analysis was done in MATLAB (The MathWorks) or SPSS (IBM). t-test, ANOVA, and mixed model were used for statistical tests. Significance level was set at α = 0.05, and p values are described in the main text or figure legends.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files. Numerical values of graphs are provided in source data files. The custom-written code files in Mathematica are uploaded as Figure 7—source code 1.

References

1. Acsády L
2. Kamondi A
3. Sík A
4. Freund T
5. Buzsáki G
(1998) GABAergic cells are the major postsynaptic targets of mossy fibers in the rat hippocampus
The Journal of Neuroscience 18:3386–3403.
https://doi.org/10.1523/JNEUROSCI.18-09-03386.1998
- PubMed
- Google Scholar
1. Acuna C
2. Liu X
3. Gonzalez A
4. Südhof TC
(2015) RIM-BPs mediate tight coupling of action potentials to Ca(2+)-triggered neurotransmitter release
Neuron 87:1234–1247.
https://doi.org/10.1016/j.neuron.2015.08.027
- PubMed
- Google Scholar
(1991) Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse
The Journal of Neuroscience 11:1496–1507.
https://doi.org/10.1523/JNEUROSCI.11-06-01496.1991
- PubMed
- Google Scholar
1. Blatow M
2. Caputi A
3. Burnashev N
4. Monyer H
5. Rozov A
(2003) Ca2+ buffer saturation underlies paired pulse facilitation in calbindin-D28k-containing terminals
Neuron 38:79–88.
https://doi.org/10.1016/s0896-6273(03)00196-x
- PubMed
- Google Scholar
1. Böhme MA
2. Beis C
3. Reddy-Alla S
4. Reynolds E
5. Mampell MM
6. Grasskamp AT
7. Lützkendorf J
8. Bergeron DD
9. Driller JH
10. Babikir H
11. Göttfert F
12. Robinson IM
13. O’Kane CJ
14. Hell SW
15. Wahl MC
16. Stelzl U
17. Loll B
18. Walter AM
19. Sigrist SJ
(2016) Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca(2+) channel-vesicle coupling
Nature Neuroscience 19:1311–1320.
https://doi.org/10.1038/nn.4364
- PubMed
- Google Scholar
(2000) Calcium sensitivity of glutamate release in a calyx-type terminal
Science 289:953–957.
https://doi.org/10.1126/science.289.5481.953
- PubMed
- Google Scholar
1. Borst JG
2. Sakmann B
(1996) Calcium influx and transmitter release in a fast CNS synapse
Nature 383:431–434.
https://doi.org/10.1038/383431a0
- PubMed
- Google Scholar
1. Brockmann MM
2. Maglione M
3. Willmes CG
4. Stumpf A
5. Bouazza BA
6. Velasquez LM
7. Grauel MK
8. Beed P
9. Lehmann M
10. Gimber N
11. Schmoranzer J
12. Sigrist SJ
13. Rosenmund C
14. Schmitz D
(2019) RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses
eLife 8:e43243.
https://doi.org/10.7554/eLife.43243
- PubMed
- Google Scholar
(2020) A trio of active zone proteins comprised of RIM-BPs, RIMs, and Munc13s governs neurotransmitter release
Cell Reports 32:107960.
https://doi.org/10.1016/j.celrep.2020.107960
- PubMed
- Google Scholar
(2021) RIM-binding protein 2 organizes Ca²⁺ channel topography and regulates release probability and vesicle replenishment at a fast central synapse
The Journal of Neuroscience 41:7742–7767.
https://doi.org/10.1523/JNEUROSCI.0586-21.2021
- PubMed
- Google Scholar
(1994) The role of Ca2+ channels in hippocampal mossy fiber synaptic transmission and long-term potentiation
Neuron 12:261–269.
https://doi.org/10.1016/0896-6273(94)90269-0
- PubMed
- Google Scholar
1. Dodge FA
2. Rahamimoff R
(1967) Co‐operative action of calcium ions in transmitter release at the neuromuscular junction
The Journal of Physiology 193:419–432.
https://doi.org/10.1113/jphysiol.1967.sp008367
- Google Scholar
1. Geiger JRP
2. Jonas P
(2000) Dynamic control of presynaptic Ca2+ inflow by fast-inactivating K+ channels in hippocampal mossy fiber boutons
Neuron 28:927–939.
https://doi.org/10.1016/S0896-6273(00)00164-1
- Google Scholar
1. Gillis KD
(2000) Admittance-based measurement of membrane capacitance using the EPC-9 patch-clamp amplifier
Pflugers Archiv 439:655–664.
https://doi.org/10.1007/s004249900173
- PubMed
- Google Scholar
1. Grauel MK
2. Maglione M
3. Reddy-Alla S
4. Willmes CG
5. Brockmann MM
6. Trimbuch T
7. Rosenmund T
8. Pangalos M
9. Vardar G
10. Stumpf A
11. Walter AM
12. Rost BR
13. Eickholt BJ
14. Haucke V
15. Schmitz D
16. Sigrist SJ
17. Rosenmund C
(2016) RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses
PNAS 113:11615–11620.
https://doi.org/10.1073/pnas.1605256113
- PubMed
- Google Scholar
(2003) A large pool of releasable vesicles in A cortical glutamatergic synapse
PNAS 100:8975–8980.
https://doi.org/10.1073/pnas.1432836100
- PubMed
- Google Scholar
(2002) RIM binding proteins (RBPs) couple Rab3-interacting molecules (RIMs) to voltage-gated Ca(2+) channels
Neuron 34:411–423.
https://doi.org/10.1016/s0896-6273(02)00667-0
- PubMed
- Google Scholar
1. Kaeser PS
2. Deng L
3. Wang Y
4. Dulubova I
5. Liu X
6. Rizo J
7. Südhof TC
(2011) RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction
Cell 144:282–295.
https://doi.org/10.1016/j.cell.2010.12.029
- PubMed
- Google Scholar
1. Krinner S
2. Butola T
3. Jung S
4. Wichmann C
5. Moser T
(2017) RIM-binding protein 2 promotes a large number of Ca_V1.3 Ca²⁺-channels and contributes to fast synaptic vesicle replenishment at hair cell active zones
Frontiers in Cellular Neuroscience 11:334.
https://doi.org/10.3389/fncel.2017.00334
- PubMed
- Google Scholar
1. Krinner S
2. Predoehl F
3. Burfeind D
4. Vogl C
5. Moser T
(2021) RIM-binding proteins are required for normal sound-encoding at afferent inner hair cell synapses
Frontiers in Molecular Neuroscience 14:651935.
https://doi.org/10.3389/fnmol.2021.651935
- PubMed
- Google Scholar
(2007) Differential gating and recruitment of P/Q-, N-, and R-type Ca2+ channels in hippocampal mossy fiber boutons
The Journal of Neuroscience 27:13420–13429.
https://doi.org/10.1523/JNEUROSCI.1709-07.2007
- PubMed
- Google Scholar
1. Lindau M
2. Neher E
(1988) Patch-clamp techniques for time-resolved capacitance measurements in single cells
Pflugers Archiv 411:137–146.
https://doi.org/10.1007/BF00582306
- PubMed
- Google Scholar
1. Liu KSY
2. Siebert M
3. Mertel S
4. Knoche E
5. Wegener S
6. Wichmann C
7. Matkovic T
8. Muhammad K
9. Depner H
10. Mettke C
11. Bückers J
12. Hell SW
13. Müller M
14. Davis GW
15. Schmitz D
16. Sigrist SJ
(2011) RIM-binding protein, a central part of the active zone, is essential for neurotransmitter release
Science 334:1565–1569.
https://doi.org/10.1126/science.1212991
- PubMed
- Google Scholar
1. Luo F
2. Liu X
3. Südhof TC
4. Acuna C
(2017) Efficient stimulus-secretion coupling at ribbon synapses requires RIM-binding protein tethering of L-type Ca²⁺ channels
PNAS 114:E8081–E8090.
https://doi.org/10.1073/pnas.1702991114
- PubMed
- Google Scholar
1. Midorikawa M
2. Sakaba T
(2017) Kinetics of releasable synaptic vesicles and their plastic changes at hippocampal mossy fiber synapses
Neuron 96:1033–1040.
https://doi.org/10.1016/j.neuron.2017.10.016
- PubMed
- Google Scholar
1. Miki T
2. Malagon G
3. Pulido C
4. Llano I
5. Neher E
6. Marty A
(2016) Actin- and myosin-dependent vesicle loading of presynaptic docking sites prior to exocytosis
Neuron 91:808–823.
https://doi.org/10.1016/j.neuron.2016.07.033
- PubMed
- Google Scholar
1. Mittelstaedt T
2. Schoch S
(2007) Structure and evolution of RIM-BP genes: identification of a novel family member
Gene 403:70–79.
https://doi.org/10.1016/j.gene.2007.08.004
- PubMed
- Google Scholar
(2015) RIM-binding protein links synaptic homeostasis to the stabilization and replenishment of high release probability vesicles
Neuron 85:1056–1069.
https://doi.org/10.1016/j.neuron.2015.01.024
- PubMed
- Google Scholar
1. Neher E
2. Brose N
(2018) Dynamically primed synaptic vesicle states: Key to understand synaptic short-term plasticity
Neuron 100:1283–1291.
https://doi.org/10.1016/j.neuron.2018.11.024
- PubMed
- Google Scholar
(2006) Compartmentalized Ca(2+) channel regulation at divergent mossy-fiber release sites underlies target cell-dependent plasticity
Neuron 52:497–510.
https://doi.org/10.1016/j.neuron.2006.08.032
- PubMed
- Google Scholar
1. Petzoldt AG
2. Götz TWB
3. Driller JH
4. Lützkendorf J
5. Reddy-Alla S
6. Matkovic-Rachid T
7. Liu S
8. Knoche E
9. Mertel S
10. Ugorets V
11. Lehmann M
12. Ramesh N
13. Beuschel CB
14. Kuropka B
15. Freund C
16. Stelzl U
17. Loll B
18. Liu F
19. Wahl MC
20. Sigrist SJ
(2020) RIM-binding protein couples synaptic vesicle recruitment to release sites
The Journal of Cell Biology 219:e201902059.
https://doi.org/10.1083/jcb.201902059
- PubMed
- Google Scholar
(2007) Structural determinants of transmission at large hippocampal mossy fiber synapses
The Journal of Neuroscience 27:10434–10444.
https://doi.org/10.1523/JNEUROSCI.1946-07.2007
- PubMed
- Google Scholar
1. Sakamoto H
2. Ariyoshi T
3. Kimpara N
4. Sugao K
5. Taiko I
6. Takikawa K
7. Asanuma D
8. Namiki S
9. Hirose K
(2018) Synaptic weight set by Munc13-1 supramolecular assemblies
Nature Neuroscience 21:41–49.
https://doi.org/10.1038/s41593-017-0041-9
- PubMed
- Google Scholar
Preprint
1. Sakamoto H
2. Kimpara N
3. Namiki S
4. Hamada S
5. Ohtsuka T
6. Hirose K
(2022) Synapse Type-Specific Molecular Nanoconfigurations of the Presynaptic Active Zone in the Hippocampus Identified by Systematic Nanoscopy
bioRxiv.
https://doi.org/10.1101/2022.03.11.483942
- Google Scholar
(1999) Released fraction and total size of a pool of immediately available transmitter quanta at a calyx synapse
Neuron 23:399–409.
https://doi.org/10.1016/s0896-6273(00)80789-8
- PubMed
- Google Scholar
1. Schneggenburger R
2. Neher E
(2000) Intracellular calcium dependence of transmitter release rates at a fast central synapse
Nature 406:889–893.
https://doi.org/10.1038/35022702
- PubMed
- Google Scholar
1. Südhof TC
(2012) The presynaptic active zone
Neuron 75:11–25.
https://doi.org/10.1016/j.neuron.2012.06.012
- PubMed
- Google Scholar
1. Tang AH
2. Chen H
3. Li TP
4. Metzbower SR
5. MacGillavry HD
6. Blanpied TA
(2016) A trans-synaptic nanocolumn aligns neurotransmitter release to receptors
Nature 536:210–214.
https://doi.org/10.1038/nature19058
- PubMed
- Google Scholar
1. Thanawala MS
2. Regehr WG
(2013) Presynaptic calcium influx controls neurotransmitter release in part by regulating the effective size of the readily releasable pool
The Journal of Neuroscience 33:4625–4633.
https://doi.org/10.1523/JNEUROSCI.4031-12.2013
- PubMed
- Google Scholar
1. von Gersdorff H
2. Matthews G
(1996) Calcium-dependent inactivation of calcium current in synaptic terminals of retinal bipolar neurons
The Journal of Neuroscience 16:115–122.
https://doi.org/10.1523/JNEUROSCI.16-01-00115.1996
- Google Scholar
1. Vyleta NP
2. Jonas P
(2014) Loose coupling between Ca2+ channels and release sensors at a plastic hippocampal synapse
Science 343:665–670.
https://doi.org/10.1126/science.1244811
- PubMed
- Google Scholar
1. Wadel K
2. Neher E
3. Sakaba T
(2007) The coupling between synaptic vesicles and Ca2+ channels determines fast neurotransmitter release
Neuron 53:563–575.
https://doi.org/10.1016/j.neuron.2007.01.021
- PubMed
- Google Scholar
(2000) The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins
The Journal of Biological Chemistry 275:20033–20044.
https://doi.org/10.1074/jbc.M909008199
- PubMed
- Google Scholar
(1997) Ultrastructural localization of zinc transporter-3 (ZnT-3) to synaptic vesicle membranes within mossy fiber boutons in the hippocampus of mouse and monkey
PNAS 94:12676–12681.
https://doi.org/10.1073/pnas.94.23.12676
- PubMed
- Google Scholar

Article and author information

Author details

Rinako Miyano

Graduate School of Brain Science, Doshisha University, Kyoto, Japan

Contribution
Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing - original draft, Writing - review and editing

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0002-0022-7198
Hirokazu Sakamoto

Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Japan

Contribution
Conceptualization, Resources, Formal analysis, Funding acquisition, Investigation, Writing - original draft, Writing - review and editing

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0002-9881-5173
Kenzo Hirose
1. Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Japan
2. International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo, Bunkyo-ku, Japan
Contribution
Conceptualization, Supervision, Funding acquisition, Investigation, Writing - review and editing

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0002-8944-6513
Takeshi Sakaba

Graduate School of Brain Science, Doshisha University, Kyoto, Japan

Contribution
Conceptualization, Supervision, Funding acquisition, Validation, Investigation, Writing - review and editing

For correspondence
tsakaba@mail.doshisha.ac.jp

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0003-0688-7717

Funding

Japan Society for the Promotion of Science (KAKENHI JP20J20550)

Rinako Miyano

Japan Society for the Promotion of Science (JSPS Core-to-Core Program A. Advanced Research Networks JPJSCCA20220007)

Takeshi Sakaba

Japan Science and Technology Agency (PRESTO JPMJPR21E7)

Hirokazu Sakamoto

Takeda Science Foundation (Bioscience and Specific Research Grants)

Takeshi Sakaba

Japan Society for the Promotion of Science (KAKENHI JP20KK0171)

Takeshi Sakaba
Hirokazu Sakamoto

Japan Society for the Promotion of Science (KAKENHI JP21K15183)

Hirokazu Sakamoto

Japan Society for the Promotion of Science (KAKENHI JP20H03427)

Kenzo Hirose

Japan Society for the Promotion of Science (KAKENHI JP21H02598)

Takeshi Sakaba

Japan Society for the Promotion of Science (KAKENHI JP22K18358)

Kenzo Hirose

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Stephan Sigrist, Dietmar Schmitz, and Christian Rosenmund for kindly providing us RIM-BP2 KO mice. We also thank Stephan Sigrist for comments on the manuscript and The IRCN Imaging Core, The University of Tokyo Institutes for Advanced Studies, for the use of STED microscopy and for assistance. This work has been supported by JSPS KAKENHI (JP20J20550 to RM, JP20KK0171, JP21K15183 to HS, JP20H03427 to KH, JP20KK0171, JP21H02598 to TS), JSPS Core-to-Core program A. Advanced Research Networks (JPJSCCA20220007 to TS), JST PRESTO (JPMJPR21E7 to HS), and Takeda Science Foundation (Bioscience and Specific Research Grants to TS).

Ethics

All animal experiments were conducted in accordance with the guidelines of the 599 Physiological Society of Japan, and were approved by Doshisha University Animal 600 Experiment Committee (A22063, D22063).

Version history

Sent for peer review: July 18, 2023
Preprint posted: July 19, 2023 (view preprint)
Preprint posted: November 9, 2023 (view preprint)
Preprint posted: January 19, 2024 (view preprint)
Version of Record published: February 8, 2024 (version 1)

Cite all versions

You can cite all versions using the DOI https://doi.org/10.7554/eLife.90799. This DOI represents all versions, and will always resolve to the latest one.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.