(A, B) Scanning electron microscopy (SEM), (C, D) transmitted light microscope, and (E, F) stereomicroscope images. White arrows show the outer organic sheath of a new chamber during its gradual …
(A) Cell-impermeable Calcein (cyan) indicating endocytotic seawater vesicles, see Figure 2—video 1. (B) FM1-43 membrane dye indicating endocytotic vesicles (red), see Figure 2—video 2. (C) …
Calcein staining indicates the occurrence of seawater vesicles inside the cytosol.
Because FM1-43 stains only the cell membrane, the observed vesicles must be originated during the process of endocytosis. The movie was taken by overlaid of 84 images during 433 s.
The movie was taken by overlaid of 37 images during 555 s.
The movie was taken by overlaid of 37 images during 555 s.
Test cross-section showing: (A, B) carbonate nanograins within organic matrix, (C, D) nanograins merging into needle-like mesocrystals, (E) fully developed needle-shaped elements; pn – nanograins …
SEM images of fixed P. eburnea.
SEM images of broken specimen of P. eburnea (d’Orbigny) showing the test wall made of calcite nanograins (A–E) within organic gel-like fluids in-between, and cytoplasmic structures below a newly …
SEM images of fixed P. eburnea.
SEM images of broken specimen of P. eburnea (d’Orbigny) showing a cross-section of the mineralized outer organic sheath of the last chamber (A, B) and the fully mineralized test wall of the former …
SEM images of fixed P. eburnea.
SEM images of different parts of the broken test of the P. eburnea (d’Orbigny) specimen showing (A) a fully developed test wall made of randomly oriented calcite needle-shaped crystals with planar …
SEM images of P. eburnea.
Scanning electron microscopy (SEM) images (A) and EDS spectra (A1–A3) of P. eburnea fixed cytosol (A1, A2) and newly created chamber (A3) indicating the chemical composition of both structures. …
(A) Outer organic sheath, (B) mineralized outer organic sheath, (C) calcite nanograins within a gel-like organic matrix, (D) needle-shaped mesocrystal growth, (E) needle-like calcite building …
SEM images of P. eburnea.
White spots labeled as Mg-ACC represent vesicles with Mg-rich amorphous calcium carbonates.
Dye | Concentration | Excitation nm | Emission nm | Source | Function |
---|---|---|---|---|---|
LysoGlow84 | 50 µM | Multiphoton 730 | 380–415/450–470 | Marnas Biochemicals | pH, membrane permeable |
FM1-43 | 1 µM | Argon 488 or Multiphoton 1000 nm | 580–620 | Thermo Fisher Scientific | Membrane staining |
Calcein | 0,7 mg/10 mL | Argon 488 | 510–555 | Thermo Fisher Scientific | Membrane -impermeable water soluble dye |
Autofluorescence | Diode 405 MP 800 | 420–490 | CaCO3, ACC | ||
Autofluorescence | Diode 405/HeNe 633 | 650–700 | Chlorophyll of algae |