(A) Left, schematic of fiber photometry with simultaneous electroencephalogram (EEG) and electromyogram (EMG) recordings. Mouse brain figure adapted from the Allen Reference Atlas - Mouse Brain. …
Related to Figure 1. (A) Left, schematic of fiber photometry with simultaneous electroencephalogram (EEG) and electromyogram (EMG) recordings. Mouse brain figure adapted from the Allen Reference …
Related to Figure 1. (A) Left, schematic of fiber photometry with simultaneous electroencephalogram (EEG) and electromyogram (EMG) recordings. Middle, fluorescence image of TMN in an HDC-Cre mouse …
(A) Left, schematic of optogenetic inhibition experiments. Center left, fluorescence image of POA in a GAD2-CRE mouse injected with AAVretro-DIO-SwiChR++-eYFP into the TMN. Scale bar, 1 mm. Center …
Related to Figure 2. (A) Duration of rapid eye movement sleep (REMs), non-rapid eye movement sleep (NREMs), and wake episodes with and without laser stimulation in SwiChR++ and eYFP mice. (B) …
Related to Figure 2. (A) Left, schematic of optogenetic inhibition experiments. Center left, fluorescence image of TMN in HDC-Cre mouse injected with AAV2-EF1a-DIO-SwiChR++-eYFP into the TMN. Scale …
(A) Schematic of REMs restriction/rebound and photometry recording experiments. The brain state was continuously monitored; once a REMs episode was detected, we used a vibrating motor (zeitgeber …
Related to Figure 3. (A) Example session during REMs restriction (top) and REMs rebound (bottom) from the same mouse. Shown are EEG spectrogram, electromyogram (EMG) amplitude, motor vibration …
Related to Figure 3. (A) Percentage of REMs, duration, and frequency of REMs episodes during REMs restriction (green, zeitgeber time [ZT] 6.5–7.5) and baseline recordings (gray, ZT 6.5–7.5). Paired …
(A) Schematic of REMs restriction/rebound and optogenetic inhibition experiments. During closed-loop REMs restriction (zeitgeber time [ZT] 1.5–7.5), a vibrating motor attached to the mouse head was …
Related to Figure 4. (A) Duration of REMs, non-rapid eye movement sleep (NREMs), and wake episodes during the last 3 hr of REMs restriction with laser stimulation (zeitgeber time [ZT] 4.5–7.5) in …
Mouse brain figure adapted from the Allen Reference Atlas - Mouse Brain.
This data is shown as bar graphs in Figure 4.
(A) Schematic of optogenetic inhibition experiments. Mouse brain figure adapted from the Allen Reference Atlas - Mouse Brain. (B) Percentage of time spent in REMs, NREMs and wakefulness with laser …
Statistical Analysis Table.