Time-lapse observations of three-dimensional (3D) reconstructed chloroplast morphology in Arabidopsis mesophyll cells accumulating chloroplast stroma–targeted fluorescent markers. A leaf from a …
Confocal images of mesophyll cells from wild-type (A) or atg7 (B) plants accumulating the chloroplast stroma marker RBCS-mRFP. Second rosette leaves of 21-day-old plants were excised and incubated …
Source data for the graph in Figure 1—figure supplement 1.
(A) Orthogonal projections produced from z-stack images (30 µm in depth) of mesophyll cells from wild-type (WT) or atg7 leaves accumulating the chloroplast stroma marker RBCS-mRFP. Second rosette …
Source data for the graph in Figure 1—figure supplement 2.
Time-lapse observations of Arabidopsis mesophyll cells accumulating the chloroplast stroma marker along with an envelope marker or a thylakoid membrane marker. Leaves accumulating stromal RBCS-GFP …
Source data for the graphs in Figure 2.
Second rosette leaves accumulating stromal RBCS-GFP along with outer envelope membrane–bound TOC64-mRFP (A), inner envelope membrane–bound KEA1-mRFP (B), or thylakoid membrane–bound ATPC1-tagRFP (C) …
Source data for the graphs in Figure 2—figure supplement 1.
Time-lapse observations of Arabidopsis mesophyll cells accumulating the chloroplast stroma marker RBCS-GFP along with inner envelope membrane–bound KEA1-mRFP. The second rosette leaf from a …
A leaf accumulating chloroplast stroma–targeted GFP (CT-GFP) was incubated in sugar-free solution in darkness for 6 hr from dawn, and the transport of an RCB was tracked. The second rosette leaf …
Source data for the graph in Figure 3.
A leaf accumulating RBCS-tagRFP (A) or RBCS-EYFP (B) was incubated in sugar-free solution in the dark for 7–8 hr from dawn, and the transport of an RCB was tracked. Second rosette leaves from …
Leaves accumulating the chloroplast stroma marker RBCS-mRFP along with the vacuolar membrane marker VHP1-mGFP were incubated in sugar-free solution in darkness for 6–8 hr from dawn, and the behavior …
Additional time-lapse data of the experiments described in Figure 4. The second rosette leaves from 21- (A) or 24-day-old (B, C) plants were used. The images in (A), (B) and (C) are still frames …
Leaves from wild-type (WT), atg5, or atg7 plants accumulating the chloroplast stroma marker RBCS-mRFP were incubated in sugar-free solution containing 1 µM concanamycin A (concA) for 1 day in …
Source data for the graphs in Figure 5.
The experiments described in Figure 5 were performed on leaves from wild-type (WT), atg2, or atg10 plants accumulating the chloroplast stroma marker RBCS-mRFP. Second rosette leaves from 21-day-old …
Source data for the graphs in Figure 5—figure supplement 1.
Leaves accumulating the chloroplast stroma marker, RBCS-mRFP (A) or CT-DsRed (B), and the isolation membrane marker GFP-ATG8a were incubated in sugar-free solution in darkness for 6 hr from dawn (A) …
Source data for the graphs in Figure 6.
(A) A leaf accumulating the chloroplast stroma marker CT-DsRed and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in darkness for 6 hr from dawn and then observed. A …
Source data for the graphs in Figure 6—figure supplement 1.
A leaf accumulating the chloroplast stroma marker RBCS-tagRFP and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in darkness for 5 hr from dawn and then observed. A …
Leaves accumulating the chloroplast stroma marker RBCS-mRFP and the isolation membrane marker GFP-ATG8a were incubated in sugar-free solution in the dark for 5–9 hr from dawn, and chloroplast …
Source data for the graphs in Figure 6—figure supplement 3.
Leaves accumulating the chloroplast stroma marker RBCS-mRFP and the isolation membrane marker GFP-ATG8a were incubated in sugar-free solution in the dark for 5–9 hr from dawn, and GFP-ATG8a-labeled …
Source data for the graph in Figure 6—figure supplement 4.
(A) Orthogonal projections produced from z-stack images (10 μm in depth) of guard cells from wild-type (WT), atg5, NahG, and atg5 NahG leaves accumulating chloroplast stroma−targeted GFP (CT-GFP). …
Source data for the graphs in Figure 7.
(A) Orthogonal projections produced from z-stack images (15 µm in depth) of mesophyll cells from leaves of wild-type (WT), atg5, and atg7 plants accumulating RBCS-mRFP. Third rosette leaves from …
Source data for the graph in Figure 7—figure supplement 1.
Orthogonal projections produced from z-stack images (15 µm in depth) of mesophyll cells from wild-type (WT), atg5, atg7, sid2, sid2 atg5, and sid2 atg7 leaves accumulating CT-GFP. Third rosette …
H2O2 contents in third rosette leaves from 36-day-old plants. Wild-type (WT) plants without any transgenic construct and CT-GFP-expressing plants in the WT, atg5, NahG, and atg5 NahG backgrounds …
Source data for the graph in Figure 7—figure supplement 3.
(A) Confocal images of mesophyll cells from wild-type (WT) and drp5b leaves accumulating the stroma marker RBCS-mRFP. Second rosette leaves from 21-day-old plants were incubated in sugar-free …
Original files for western blot analysis displayed in Figure 8C.
PDF file containing original western blots for Figure 8C, indicating the relevant bands, genotypes, and conditions.
Source data for the graphs in Figure 8.
(A) Confocal images of mesophyll cells from wild-type (WT), atg5, drp5b, and drp5b atg5 leaves accumulating the stroma marker CT-GFP. Second rosette leaves from 21-day-old plants were incubated in …
Source data for the graph in Figure 8—figure supplement 1.
(A) Confocal images of mesophyll cells from wild-type (WT), drp5b, atg5, and atg7 leaves accumulating the stroma marker RBCS-mRFP. Second rosette leaves from 21-day-old plants were incubated in …
Source data for the graph in Figure 8—figure supplement 2.
(A) A leaf from the drp5b mutant accumulating the stroma marker RBCS-mRFP was incubated in sugar-free solution in darkness for 5 hr from dawn and then observed. Arrowheads indicate a chloroplast …
(A) Individual channel images (CT-GFP and chlorophyll) from the merged orthogonal projection image shown in the previous version of Figure 7–figure supplement 1. The right panel shows the enhanced …
The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma–targeted GFP (CT-GFP) was incubated in sugar-free solution in darkness for time-lapse imaging with a two-photon …
The second rosette leaf from a 22-day-old plant accumulating the chloroplast stroma marker RBCS-mRFP was incubated in sugar-free solution in darkness for time-lapse imaging with a two-photon …
The second rosette leaf from a 20-day-old plant accumulating the chloroplast stroma marker RBCS-EYFP was incubated in sugar-free solution in darkness for time-lapse imaging with a two-photon …
The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma marker RBCS-GFP and the outer envelope marker TOC64-mRFP was incubated in sugar-free solution in darkness for …
The second rosette leaf from a 22-day-old plant accumulating the chloroplast stroma marker RBCS-GFP and the inner envelope marker KEA1-mRFP was incubated in sugar-free solution in the dark for …
The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma marker RBCS-GFP and the thylakoid membrane marker ATPC1-tagRFP was incubated in sugar-free solution in darkness …
The second rosette leaf from a 21-day-old plant accumulating chloroplast stroma–targeted GFP (CT-GFP) was incubated in sugar-free solution in darkness for time-lapse monitoring of an RCB marked by …
The second rosette leaf from a 22-day-old plant accumulating stromal RBCS-tagRFP was incubated in sugar-free solution in the dark for time-lapse monitoring of an RCB marked by RBCS-tagRFP. Arrowhead …
The second rosette leaf from a 22-day-old plant accumulating stromal RBCS-EYFP was incubated in sugar-free solution in the dark for time-lapse monitoring of an RCB marked by RBCS-EYFP. Arrowhead …
The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma marker RBCS-mRFP along with the vacuolar membrane marker VHP1-mGFP was incubated in sugar-free solution in …
The second rosette leaf from a 23-day-old plant accumulating the chloroplast stroma marker RBCS-mRFP and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in darkness for …
The second rosette leaf from a 23-day-old plant accumulating the chloroplast stroma–targeted DsRed (CT-DsRed) and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in …
The second rosette leaf from a 22-day-old plant accumulating the chloroplast stroma–targeted DsRed (CT-DsRed) and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in …
The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma marker RBCS-tagRFP and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in darkness …
The second rosette leaf from a 21-day-old drp5b plant accumulating the chloroplast stroma marker RBCS-mRFP was incubated in sugar-free solution in darkness for time-lapse imaging. Arrowhead …
The second rosette leaf from a 22-day-old drp5b plant accumulating the chloroplast stroma marker RBCS-mRFP and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Arabidopsis thaliana) | RBCS2B | TAIR | AT5G38420 | |
Gene (A. thaliana) | VHP1 | TAIR | AT1G15690 | |
Gene (A. thaliana) | TOC64-III | TAIR | AT3G17970 | |
Gene (A. thaliana) | ATG8a | TAIR | AT4G21980 | |
Gene (A. thaliana) | ATPC1 | TAIR | AT4G04640 | |
Gene (A. thaliana) | KEA1 | TAIR | AT1G01790 | |
Genetic reagent (A. thaliana) | atg5-1 | ABRC | SAIL_129_B07 | |
Genetic reagent (A. thaliana) | atg7-2 | ABRC | GABI_655B06 | |
Genetic reagent (A. thaliana) | atg2-1 | ABRC | SALK_076727 | |
Genetic reagent (A. thaliana) | atg10-1 | ABRC | SALK_084434 | |
Genetic reagent (A. thaliana) | drp5b (arc5-2) | ABRC | SAIL_71_D11 | |
Genetic reagent (A. thaliana) | sid2-2 | ABRC | CS16438 | |
Genetic reagent (A. thaliana) | NahG atg5-1 | 10.1105/tpc.109.068635 | ||
Genetic reagent (A. thaliana) | sid2-2 atg5-1 | 10.1105/tpc.109.068635 | ||
Genetic reagent (A. thaliana) | Pro35S:CT-GFP | 10.1126/science.276.5321.2039 | ||
Genetic reagent (A. thaliana) | Pro35S:CT-DsRed | 10.1093/pcp/pcab084 | ||
Genetic reagent (A. thaliana) | ProRBCS:RBCS-GFP | 10.1104/pp.108.122770 | ||
Genetic reagent (A. thaliana) | ProVHP1:VHP1-mGFP | 10.1105/tpc.114.127571 | ||
Genetic reagent (A. thaliana) | ProUBQ:GFP-ATG8a | 10.1093/pcp/pcaa162 | ||
Genetic reagent (A. thaliana) | ProTOC64:TOC64-mRFP | 10.26434/chemrxiv-2023-kx6gp | ||
Genetic reagent (A. thaliana) | ProRBCS:RBCS-EYFP | This paper | See ‘Plant materials’ in Materials and methods | |
Genetic reagent (A. thaliana) | ProRBCS:RBCS-mRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Genetic reagent (A. thaliana) | ProRBCS:RBCS-tagRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Genetic reagent (A. thaliana) | ProATPC1:ATPC1-tagRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Genetic reagent (A. thaliana) | ProKEA1:KEA1-mRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Antibody | Anti-RFP (Mouse, monoclonal) | MBL | M204-3 | (1:2000) |
Antibody | Anti-cFBPase (Rabbit, polyclonal) | Agrisera | AS04043 | (1:5000) |
Recombinant DNA reagent | ProRBCS:RBCS-EYFP | This paper | See ‘Plant materials’ in Materials and methods | |
Recombinant DNA reagent | ProRBCS:RBCS-mRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Recombinant DNA reagent | ProRBCS:RBCS-tagRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Recombinant DNA reagent | ProATPC1:ATPC1-tagRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Recombinant DNA reagent | ProKEA1:KEA1-mRFP | This paper | See ‘Plant materials’ in Materials and methods | |
Sequence-based reagent | ATPC1_F | This paper | PCR primers (cloning) | CACCCATGGAGAGGGCTCGTACCTTAC |
Sequence-based reagent | ATPC1_R | This paper | PCR primers (cloning) | AACCTGTGCATTAGCTCCAG |
Sequence-based reagent | KEA1_F | This paper | PCR primers (cloning) | AGGAACCAATTCAGTCGACTCATGATCATAACAAGTCTC |
Sequence-based reagent | KEA1_R | This paper | PCR primers (cloning) | AAAGCTGGGTCTAGATATCCGATTACGACTGTGCCTCCTTC |
Commercial assay or kit | Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit | Invitrogen | A22188 | |
Chemical compound, drug | Concanamycin A | Santa Cruz | sc-202111 | |
Software, algorithm | ZEN | Carl Zeiss | RRID:SCR_013672 | Image processing and quantification (microscopy) |
Software, algorithm | NIS-Elements C | Nikon | RRID:SCR_020318 | Image processing and quantification (microscopy) |
Software, algorithm | LAS X | Leica | RRID:SCR_013673 | Image processing and quantification (microscopy) |
Software, algorithm | Imaris | Bitplane | RRID:SCR_007370 | Image processing and quantification (microscopy) |
Software, algorithm | Image lab | Bio-Rad | RRID:SCR_014210 | Image processing and quantification (western blot) |
Software, algorithm | JMP14 | SAS | RRID:SCR_022199 | Statistics |