(A) Temporal changes in growth rate. Color variation indicates the nine evolutionary lineages. (B) Growth rate and maximal population size of the reduced genome. Blue and pink indicate the common …
Four different dilution rates of serial transfer were made for each evolutionary lineage, indicated in the blue gradation. Only one of the four overnight cultures in the exponential growth phase (OD6…
The nine evolutionary lineages are shown separately, equivalent to Figure 1A. The red lines are the logarithmic regression of the changing growth rates.
(A) Normalized genomic positions of all mutations. The vertical lines highlight the total 65 mutations fixed in the nine Evos. Color variation indicates the nine Evos. WT and reduced represent the …
(A) Chromosomal periodicity of transcriptomes. The transcriptomes of the nine Evos are shown. Black lines, red curves, and red vertical lines indicate the gene expression levels, fitted periods, and …
(A) Commonality of DEGs in the nine Evos. Closed circles represent the combinations of the Evos. Vertical and horizontal bars indicate the number of the overlapped DEGs in the combinations and the …
(A) Hierarchical clustering. (B) Principal component analysis. PC1 and PC2 components are shown. Black, blue, and pink represent the wild-type, reduced, and evolved genomes, respectively.
(A) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. (B) Gene Ontology (GO) terms. The number of the Evos sharing the same enriched functions is shown.
(A) Venn diagrams of differentially expressed genes (DEGs) induced by genome reduction and evolution. The number of individual and overlapped DEGs is indicated. (B) Heatmap of enriched regulons. …
The number of individual and overlapped DEGs induced by genome reduction and evolution is indicated.
(A) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. (B) Gene Ontology (GO) term. GR and Evos indicate the genome reduction and evolution, respectively. The statistical significance (false …
(A) Cluster dendrogram of the gene modules reconstructed by weighted gene co-expression network analysis (WGCNA). A total of 21 gene modules (M1–M21) were reconstructed. The significance of the …
(A) Gene modules clustered by weighted gene co-expression network analysis (WGCNA). (B) Gene modules reconstructed by WGCNA. A total of 21 modules newly constructed are represented in gradation. The …
Three evolutionary strategies are proposed. Pink and blue arrowed lines indicate experimental evolution and genome reduction, respectively. The size of the open cycles represents the genome size. …
The number of mutations in the nine Evos is shown separately and summed. SNP, N, and S indicate single nucleotide substitution, nonsynonymous, and synonymous SNP, respectively.
Evos | All | Intergenic | Genic | Genic SNP | ||
---|---|---|---|---|---|---|
Indel | SNP | N | S | |||
A1 | 3 | 0 | 0 | 2 | 1 | 1 |
A2 | 2 | 1 | 0 | 1 | 1 | 0 |
B2 | 13 | 0 | 1 | 11 | 9 | 2 |
C2 | 11 | 1 | 0 | 9 | 8 | 1 |
D2 | 7 | 0 | 1 | 5 | 4 | 1 |
E2 | 3 | 1 | 0 | 1 | 1 | 0 |
F2 | 8 | 1 | 0 | 6 | 5 | 1 |
G2 | 12 | 2 | 2 | 6 | 4 | 2 |
H2 | 6 | 0 | 2 | 4 | 3 | 1 |
Sum | 65 | 6 | 6 | 45 | 36 | 9 |
Daily records of experimental evolution.
The time and OD600 of overnight culture, the well (dilution rate) used for the serial transfer, and the calculated generation and growth rate are shown. All nine evolutionary lineages are summarized.
Statistics of genome sequencing.
The parameters acquired in the genome resequencing, which represent the goodness of the sequencing, are summarized.
List of genome mutations.
The total 65 mutations fixed in the Evos are summarized. The type of mutation, the position in the reduced genome, distance to the nearest genomic scar, changes in DNA and amino acid, gene function, essentiality (e, essential; n, nonessential), gene category, etc., are indicated. Note that the entire population held the mutations, i.e., 100% frequency in DNA sequencing.
Gene categories comprising the mutated genes.
The full names and abbreviations of the gene categories are shown. The number of mutated genes in each gene category is tallied for each Evos.
Statistics of chromosomal periodicity of transcriptomes.
The maximal peak (wavelength) acquired by the Fourier transform, the number of periods resulting from the curve fitting, the Fisher’s g test, and the p-values are summarized.
List of the overlapped DEGs.
A total of 108 DEGs are summarized. Gene ID, gene name, and gene function are indicated.
Datasheet of normalized gene expression.
Gene expression levels are shown in the logarithmic value of FKPM. Gene ID, gene name, strain name, and nine evolutionary lineages are indicated. N0 and N28 represent the wild-type and reduced genomes, respectively.