SCC3 is an axial element essential for homologous chromosome pairing and synapsis
- Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, China
- State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, China
- College of Horticulture Science and Engineering, Shandong Agricultural University, China
Figures
Figure 1 with 5 supplements
Sister chromatid cohesion 3 (SCC3) is required for sister chromatid cohesion during mitosis.
(A) Morphology of seedlings and root tips in wild-type and scc3. (B) Morphology of plants in wild-type and scc3. (C) Morphology of panicles in wild-type and scc3. (D) Pollen grains stained in 1% I2-K…
Figure 1—figure supplement 1
Multiple sequence alignment of sister chromatid cohesion 3 (SCC3) with its homologs from three different species.
(A) SCC3 contains a conserved STAG domain. (B) STAG domains are conserved in both monocotyledonous and dicotyledonous plants.
Figure 1—figure supplement 2
Protein structure and phylogenetic tree analysis of sister chromatid cohesion 3 (SCC3).
(A) The full-length (1–1,116 aa) SCC3 protein among different species shows high structural similarity. The arrow indicates similarity in SCC3 subunits. (B) Phylogenetic tree derived from …
Figure 1—figure supplement 3
Multiple sequence alignment of sister chromatid cohesion 3 (SCC3) with its homologs from different species analyzed in an evolutionary tree.
Figure 1—figure supplement 4
Schematic representation of sister chromatid cohesion 3s (SCC3’s) mutation site.
Schematic representation of the SCC3 gene and its mutation site. Coding regions are shown as black boxes and untranslated regions are shown as gray boxes.
Figure 1—figure supplement 5
The expression pattern of sister chromatid cohesion 3 (SCC3) and plant phenotypic statistics of wild-type and scc3 mutants.
(A) The expression pattern of SCC3. P2, 2 cm long panicles; P2-3, 2–3 cm long panicles; P3-5, 3–5 cm long panicles; P5-7, 5–7 cm long panicles; P7, 7 cm long panicles. Expression values represent …
Figure 2 with 1 supplement
Sister chromatid cohesion 3 (SCC3) alters the structure of sister chromatids during mitosis.
(A) The dynamic process of chromosome structure in early mitosis as revealed by pooled oligos specific to chromosome 12 (red). In the wild-type, S1 and S2 indicate the replicated sister chromatids. …
Figure 2—figure supplement 1
Immunolocalization of sister chromatid cohesion 3 (SCC3) in scc3 mitosis and mitosis.
(A) Immunolocalization of CENH3 (red, from rabbit) and SCC3 (green, from mouse) in scc3 root tip cells during mitosis. Chromosomes were stained with 4',6-diamidino-2-phenylindole (DAPI). Bar, 5 μm. …
Figure 3 with 1 supplement
Sister chromatid cohesion 3 (SCC3) is an axial element during meiosis.
(A) In meiosis, SCC3 (green, from mouse) colocalizes with REC8 (red, from rabbit) from the leptotene to diplotene. Bars, 5 μm. (B) During diakinesis and metaphase I, SCC3 (green, from mouse) …
Figure 3—figure supplement 1
Immunolocalization of CENH3 and REC8 in wild-type from diakinesis to metaphase I.
Figure 4
Sister chromatid cohesion 3 (SCC3) is required for sister chromatid cohesion in early meiosis.
(A) Meiocyte chromosome morphology at the preleptotene and leptotene in wild-type, scc3, rec8, and rec8 scc3. Chromosomes at preleptotene exhibited a hairy rodlike appearance and formed thin threads …
Figure 5 with 2 supplements
Homologous pairing and synapsis are disturbed in sister chromatid cohesion 3 (scc3).
(A) Meiotic chromosome behavior in wild-type and scc3. Bars, 5 μm. (B) Immunolocalization of ZEP1 (green, from mouse) and REC8 (red, from rabbit) in wild-type and scc3 meiocytes. ZEP1 was severely …
Figure 5—figure supplement 1
Chromosome behaviors of spo11 and cytogenetic analysis of homologous pairing in wild-type and sister chromatid cohesion 3 (scc3).
(A) Chromosome behavior of spo11 in metaphase I and anaphase I. Bars, 5 μm. (B) Telomere bouquets were detected in both wild-type and scc3 with the telomere-specific probe (pAtT4, green) by …
Figure 5—figure supplement 2
Immunolocalization of ZEP1, PAIR2, and PAIR3 in sister chromatid cohesion 3 (scc3) at zygotene.
Bar, 5 μm.
Figure 6
Sister chromatid cohesion 3 (SCC3) affects recombination progress and crossover (CO) formation.
(A) Immunolocalization of histone γH2AX (red), COM1 (green, from mouse), DMC1 (green, from mouse), RAD51 (green, from rabbit), ZIP4 (green, from mouse), and HEI10 (green, from mouse) in wild-type …
Figure 7 with 3 supplements
Sister chromatid cohesion 3 (SCC3) loading onto meiotic chromosomes depends on REC8.
(A) Immunolocalization of γH2AX (red, from rabbit), COM1 (green, from mouse), RAD51 (green, from rabbit), SCC3 (green, from mouse), PAIR2 (green, from rabbit), and PAIR3 (green, from rabbit) in rec8 …
Figure 7—figure supplement 1
Sister chromatid cohesion 3 (SCC3) does not interact with REC8, SMC1, or SMC3.
(A) SCC3 does not interact with REC8, SMC1, and SMC3 in yeast-two-hybrid assays. (B) SCC3 does not interact with REC8, SMC1, and SMC3 in bimolecular fluorescence complementation assays in rice …
Figure 7—figure supplement 2
The expression levels of cohesin-related genes in wild-type and other mutants.
(A) The expression levels of SMC1, SMC3, SCC1, REC8, PAIR2, PAIR3, SGO1 and ZEP1 in sister chromatid cohesion 3 (scc3) and wild-type. Values are shown as mean ± SD of three biological replicates. * …
Figure 7—figure supplement 3
Sister chromatid cohesion 3 (SCC3) acts as the cohesin and promotes homologous pairing and synapsis.
(A) Structure model of the cohesin complex. The SCC3 subunit is associated with the middle region of SCC1. (B) During meiosis, SCC3’s localization pattern probably changes to the root of DNA loop …
Additional files
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Supplementary file 1
List of primers used in this study.
- https://cdn.elifesciences.org/articles/94180/elife-94180-supp1-v1.docx
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MDAR checklist
- https://cdn.elifesciences.org/articles/94180/elife-94180-mdarchecklist1-v1.pdf
