Distinct catecholaminergic pathways projecting to hippocampal CA1 transmit contrasting signals during navigation in familiar and novel environments
- The Department of Neurobiology, The University of Chicago, United States
Figures
Figure 1 with 3 supplements
Distinct activity dynamics in ventral tegmental area (VTA) and locus coeruleus (LC) axons during navigation of familiar environments.
(a) Experimental setup (top), created with BioRender.com. Example virtual reality environment. (b) Schematic representation of injection procedure (left). Representative coronal brain sections …
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Figure 1—source data 1
Fluorescence data of ventral tegmental area (VTA) and locus coeruleus (LC) axons in familiar virtual reality (VR) environments.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
LC and VTA axon activity as a function of time and distance to reward.
(a, i) Population activity s.e.m. binned by the virtual distance to reward for ventral tegmental area (VTA) regions of interest (ROIs) (orange, 200 m track n = 9 ROIs form 8 sessions in 8 mice) …
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Figure 1—figure supplement 1—source data 1
Fluorescence data for ventral tegmental area (VTA) and locus coeruleus (LC) axons aligned by time and distance to reward.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig1-figsupp1-data1-v1.xlsx
Figure 1—figure supplement 2
VTA DA axons expressing axon-GCaMP6s or axon-GCaMP7b show the same trends as a function of behavioral variables.
(a) Population average position binned of ventral tegmental area (VTA) GCaMP6s regions of interest (ROIs) (orange, n = 5 ROIs in 4 mice) and VTA GCaMP7b ROIs (dark orange, n = 4 ROIs, in 4 mice) …
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Figure 1—figure supplement 2—source data 1
Fluorescence data of GCaMP6s and GCaMP7b ventral tegmental area (VTA) axons.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig1-figsupp2-data1-v1.xlsx
Figure 1—figure supplement 3
Distinct activity dynamics in VTA DA axons expressing axon-GCaMP6s and LC axons expressing axon-GCaMP6 during navigation of familiar environments.
(a, i) Population average position binned s.e.m. in axon-GCaMP6s expressing ventral tegmental area (VTA) regions of interest (ROIs) (orange, n = 5 ROIs in 4 mice) and locus coeruleus (LC) ROIs …
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Figure 1—figure supplement 3—source data 1
Fluorescence data of GCaMP6s ventral tegmental area (VTA) and locus coeruleus (LC) axons in familiar virtual reality (VR) environments.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig1-figsupp3-data1-v1.xlsx
Figure 2
Removal of reward restructures ventral tegmental area (VTA) but not locus coeruleus (LC) input activity during spatial navigation.
(a) Experimental paradigm. (b, i) Population average position binned of VTA regions of interest (ROIs) (n = 6 ROIs in 6 mice) in the rewarded (VTA rew, orange) and unrewarded conditions (VTA …
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Figure 2—source data 1
Fluorescence data of ventral tegmental area (VTA) and locus coeruleus (LC) axons in rewarded and unrewarded virtual reality (VR) environments.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig2-data1-v1.xlsx
Figure 3 with 2 supplements
Exposure to a novel environment causes an abrupt and sustained increase in activity in locus coeruleus (LC) but not ventral tegmental area (VTA) inputs to dCA1.
(a, i) Experimental paradigm. (ii) Behavior from example mouse during the transition from the familiar virtual reality (VR) environment to a novel VR environment showing the animals track position …
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Figure 3—source data 1
Fluorescence data of ventral tegmental area (VTA) and locus coeruleus (LC) axons in novel virtual reality (VR) environments.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig3-data1-v1.xlsx
Figure 3—figure supplement 1
Reward related activity in VTA DA axons is diminished in a novel environment.
(a) Population average position binned of ventral tegmental area (VTA) regions of interest (ROIs) (n = 7 ROIs in 7 mice) in the familiar (orange) and novel (green) rewarded environments. Linear …
Figure 3—figure supplement 2
Increased LC axon activity following exposure to a familiar environment and during immobile periods in a novel environment.
(a, i) Mean normalized fluorescence of locus coeruleus (LC) regions of interest (ROIs) (n = 50, 11 sessions in 9 mice) aligned to the switch from dark to the familiar environment. To define a …
Figure 4
Novelty-induced changes in behavior explain the late but not early increases in locus coeruleus (LC) activity.
(a) Good behavior from example mouse following removal of freezing periods (velocity <0.2 cm/s) during the transition from the familiar to a novel virtual reality (VR) environment showing the …
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Figure 4—source data 1
Fluorescence data of locus coeruleus (LC) axons during good behavior in novel virtual reality (VR) environments.
- https://cdn.elifesciences.org/articles/95213/elife-95213-fig4-data1-v1.xlsx
