Figure 3. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 3.

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California Institute of Technology, United States
Figure 3.
Download figureOpen in new tabFigure 3. dbh mutants and bopindolol-treated WT larave exhibit lower arousal threshold.

Stimulus-response curves generated by a tapping assay for dbh−/ and sibling control larvae (A), and bopindolol and DMSO treated WT larvae (B). Thirty trials were performed at each stimulus intensity, with a 1 min inter-trial interval. Each data point indicates mean ± s.e.m. Stimulus-response curves were constructed using the non-linear variable slope module in Prism and fitted using ordinary least squares. Dashed lines mark the ETP50 values for different genotypes and drug treatments. dbh−/ have an ETP50 value of 2.1 vs 3.1 for sibling controls (32% decrease, p < 0.0001 by extra sum-of-squares F test) (A), and bopindolol treated animals have an ETP50 value of 2.6 vs 5.7 for sibling controls (54% decrease, p < 0.0001 by extra sum-of-squares F test) (B). n indicates number of larvae.

DOI: http://dx.doi.org/10.7554/eLife.07000.010