Figure 4—figure supplement 3. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 4—figure supplement 3.

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California Institute of Technology, United States
Figure 4—figure supplement 3.
Download figureOpen in new tabFigure 4—figure supplement 3. Reduced sleep at night due to Hcrt overexpression is suppressed by prazosin.

(A, C) Sleep traces from a single representative experiment; yellow region indicates time of HS during day 6. (B, D) Bar graphs show mean ± s.e.m. percentage change in sleep during night 6 compared to night 5 from two combined experiments. (B) In the absence of the hsp:Hcrt transgene, HS during day 6 has no significant effect on the Sleep Night 6/Night 5 ratio, whether the animals are treated with prazosin or vehicle. (D) The reduced sleep induced by Hcrt overexpression is significantly diminished by prazosin. n indicates number of larvae. **, p < 0.01 and ns, not significant by one-way ANOVA.

DOI: http://dx.doi.org/10.7554/eLife.07000.014