Figure 2—figure supplement 3. | The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

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The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

Figure 2—figure supplement 3.

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Cornell University, United States
Figure 2—figure supplement 3.
Download figureOpen in new tabFigure 2—figure supplement 3. MALS traces of S. cerevisiae and T. terrestris Sec7 constructs. 

Normalized gel filtration peak profiles of all Sec7 constructs are shown with MALS molecular weight measurements superimposed. The right-hand molecular weight axis for each panel is scaled to each construct’s calculated mass, with monomeric, dimeric, and trimeric masses indicated. Due to the wide variation in construct size, a single gel filtration column was not used for every construct, leading to the variation in elution profiles seen.

DOI: http://dx.doi.org/10.7554/eLife.12411.011