Figure 3. | The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

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The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

Figure 3.

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Cornell University, United States
Figure 3.
Download figureOpen in new tabFigure 3. The HDS4 domain of Sec7 is important for function but dispensable for TGN localization. 

(A) Centromeric plasmids encoding GFP-Sec7 constructs expressed from the SEC7 promoter were introduced into a SEC7 plasmid shuffling strain (CFY409). Growth on 5-FOA measures the ability of the construct to complement the sec7∆ mutation. (B) The same plasmids were imaged in an otherwise wild-type strain expressing endogenously tagged Sec7-RFPMars (‘Sec7-RFP’) (CFY578).

DOI: http://dx.doi.org/10.7554/eLife.12411.012