Figure 2—figure supplement 1. | Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

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Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

Figure 2—figure supplement 1.

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Yale University, United States; University of Georgia, United States; University of Connecticut, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Properties of the co-culture and its relationship to Drosophila preference.

(A) pH of experimental groups as a function of microbial growth time. Mean pH ± SEM of three experiments with one pooled replicate per experiment. (B) Relationship between A. malorum populations and Drosophila preference for the co-culture versus the separate-culture mixture. Each data point represents viable A. malorum populations in the co-culture along with the mean RI behavioral value toward the co-culture containing A. malorum. A semi-log standard curve with an unconstrained slope was generated and compared to a null model with slope = 0. The data do not fit to an unconstrained slope better than to the null slope = 0 model (p=0.1132). (C) Drosophila attraction to the co-culture versus sterile media as a function of co-culture age (grown 34 hr – 127 hr). Mean RI ± SEM of three experiments with 16–18 total replicates. A one-sample t-test assessed whether the group means were different from 0. NS p>0.05; *p≤0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001 Significance is denoted beside or within bars of each experimental group. ACV = apple cider vinegar (25% in water); PO = paraffin oil. (D) Behavior of Drosophila toward the co-culture grown for 72 hr versus S. cerevisiae alone, A. malorum alone, or the separate-culture mixture grown for different periods of time that correspond to different stages of growth (e.g. late log, stationary; see [E]). Mean ± SEM of 11–12 replicates in two experiments. A one-sample t-test compared the experimental group means to 0. (E) Viable populations of conditions in (D) Mean ± SEM of 2 pooled replicates where each replicate contains two replicate cultures.

DOI: http://dx.doi.org/10.7554/eLife.18855.012

Figure 2—figure supplement 1—source data 1.Raw Drosophila preference data and microbial population data for Figure 2—figure supplement 1D,E.

DOI: http://dx.doi.org/10.7554/eLife.18855.013

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