Synergistic interactions with PI(4,5)P2 and activated Rho1, which are enriched at the apical membrane of tubular organs, facilitate tight apical targeting of the formin Diaphanous.
A novel role of endosomal machineries retromer and ESCRT in membrane delivery of Drosophila septate junction components is required for maintenance of junction integrity and epithelial barrier function.
Pierre Mangeol, Dominique Massey-Harroche ... André Le Bivic
Proteins pivotal to epithelial polarization organize in separated clusters, simplifying the current view by showing that a small fraction of the many protein–protein interactions proposed are prominent in mature epithelia.
Polarity cascade initiated by aPKC in the periderm is transduced by adherens junction component E-cadherin to the basal epidermis during the development of zebrafish bilayered epidermis.
Stefan Harmansa, Ilaria Alborelli ... Markus Affolter
Functionalized protein binders, anchored to specific regions along the cell cortex, provide a novel approach to directly modify protein localization and dispersal in vivo.
Drosophila enteroblasts form septate junctions, an apical domain and an internal lumen inside the midgut epithelium before they reach the gut surface, thereby maintaining the intestinal barrier as they integrate.
Par3 is polarized in the plane of the vertebrate neural plate, binds and recruits Prickle3 to the apical membrane and promotes the formation of core planar cell polarity complexes.
Austin T Baldwin, Juliana H Kim ... John B Wallingford
Tissue-level cell tracking and analysis of actomyosin and N-cadherin adhesion dynamics, coupled with disruption of the regulatory gene Shroom3, reveals region-specific cell behaviors along the anteroposterior axis during neural tube closure.
Alexandre Francou, Kathryn V Anderson, Anna-Katerina Hadjantonakis
High-resolution live and fixed tissue imaging combined with cell and tissue scale data analyses revealed that a ratchet-like constriction of the apical surfaces of cells drives the first step of an epithelial-to-mesenchymal transition taking place during mouse gastrulation.
Olga Martzoukou, Sotiris Amillis ... George Diallinas
In filamentous fungi the AP-2 complex, which in mammals is an adaptor of clathrin-mediated endocytosis, is recruited to specific clathrin-independent apical endocytosis necessary for proper lipid maintenance and polar growth.
