Abstract
Cell crowding is a common microenvironmental factor that affects various disease processes, but its impact on cell invasiveness into surrounding tissues is not well understood. In this study, we investigated the biomechanical changes induced by cell crowding, focusing on pro-invasive cell volume reduction. We discovered that cell crowding enhanced the invasiveness of high-grade ductal carcinoma in situ (DCIS) cells, which experienced significant cell volume reduction compared to hyperplasia-mimicking or normal cells. Mass spectrometry analyses revealed that cell crowding relocated ion channels, including TRPV4, a calcium-permeant ion channel, to the plasma membrane selectively in high-grade DCIS cells but not in less aggressive or normal cells. Cell crowding inhibited TRPV4 in high-grade DCIS cells, which led to decreased intracellular calcium levels and subsequent volume reduction. TRPV4 inhibition also prompted relocation of TRPV4 to the plasma membrane. This relocation primed inactive TRPV4 for activation, effectively counterbalancing the calcium loss from crowding-induced channel inhibition. Analyses of patient-derived breast cancer tissues validated that TRPV4 selectively associated with the plasma membrane in high-grade DCIS but not in lower-grade DCIS or less aggressive pathologies. The extent of plasma membrane TRPV4 association scaled with cell volume reduction and increased cell invasiveness and motility, suggesting its utility as an active pro-invasive mechanotransduction pathway indicator. Additionally, hyperosmotic conditions and pharmacologic TRPV4 inhibition mimicked the pro-invasive volume reduction observed under cell crowding, while TRPV4 activation reversed this effect by inducing cell volume increase. In summary, our study reveals a previously unrecognized pro-invasive mechanotransduction pathway triggered by cell crowding, which is selective in high-grade DCIS cells. This discovery offers new biophysical perspectives on cell invasiveness, highlighting the critical role of a selective mechanotransduction mechanism in the progression of breast cancer cells that are considered non-invasive but associated with high risk.
eLife assessment
In this important study, Bu et al investigate how cell overcrowding triggers a mechano-transduction pathway involving TRPV4 channels, focusing on high-grade ductal carcinoma in situ (DCIS) cells. The authors show that cell crowding in these malignant cells leads to a reduction in cell volume and promotes a pro-invasive phenotype through calcium homeostasis and TRPV4 channel trafficking to the plasma membrane; this phenomenon is specific to invasive cell lines like MCF10CA and DCIS and is corroborated by patient tissue samples. The work suggests the role of TRPV4 in cell motility and mechanical sensing, offering potential therapeutic insights for targeting cancer metastasis. While the study presents robust and convincing data, the absence of TRPV4 genetic ablation is a critical limitation, which would further confirm its role in these processes.
Significance of findings
important: Findings that have theoretical or practical implications beyond a single subfield
- landmark
- fundamental
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- valuable
- useful
Strength of evidence
convincing: Appropriate and validated methodology in line with current state-of-the-art
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Introduction
The complex interplay between cellular mechanics and invasive behaviors is crucial to understanding various physiological and pathological processes, such as wound healing1 and disease progression2,3. Numerous studies show that these processes are mediated by mechanotransduction, whereby cells translate mechanical stimuli into cellular activity2,4–6. While mechanotransduction is traditionally studied concerning fluid stress7,8, matrix stiffness4,9, and other biomechanical changes such as osmotic stress10,11, the role of cell crowding, characterized by increased cell density and spatial constraints, is relatively less explored. A recent report shows that cell crowding plays a role in facilitating wound closure and repair by enhancing cell proliferation12. As tissues develop, repair, or undergo pathological transformation, cell crowding becomes common13,14. This challenges individual cells, forcing them to perceive and respond to the mechanical constraints of a crowded environment. Our study used human breast cell line model systems to describe a novel mechanotransduction pathway triggered by cell crowding that induces invasiveness into surrounding tissues. Interestingly, this pathway exhibited unique selectivity, as it specifically associated with a type of non-invasive cancer pathology and was not present in lower-grade or less aggressive pathologies. This suggests that not all cells possess the ability to translate mechanical stimuli, such as cell crowding, into cell invasiveness.
To assess the role of cell crowding in cell invasiveness, we chose in vitro cell lines linked to different pathological states that reflect cell crowding conditions in vivo, including atypical ductal hyperplasia (ADH)15 and ductal carcinoma in situ (DCIS)16–18. ADH is an intraductal clonal epithelial cell proliferative lesion18 and represents an intermediate step between normal breast tissue and in situ carcinomas19–21. ADH is associated with high risk as it is reported that an ADH diagnosis is associated with a five-fold increased risk of breast cancer15. DCIS is non-invasive form of cancer characterized by proliferating malignant epithelial cells16–18. Unlike ADH, DCIS is considered a precursor to invasive breast cancer22. However, the mechanism of how DCIS transitions to invasive cancer is not well understood and therefore, there is currently no reliable and robust method to differentiate which DCIS cells are at high risk of becoming invasive23,24.
Both ADH and DCIS conditions can potentially expose cells to crowding in vivo. However, how ADH and DCIS cells respond to such changes in cell density remains unknown. Our study revealed that cell crowding selectively triggered a pro-invasive mechanotransduction program in a specific type of DCIS cell line associated with high-grade comedo-type pathology25,26. The mechanotransduction program induced by cell crowding involved the inhibition of ion channels, such as transient receptor potential vanilloid 4 (TRPV4)27, a calcium-permeable ion channel, as identified from our mass spectrometry assay. This inhibition prompted the relocation of TRPV4 and other ion channels to the plasma membrane, leading to decreased intracellular calcium levels, subsequent induction of reduced cell volume and cortical stiffening, thereby promoting cell invasiveness.
We confirmed that inhibition of TRPV4 led to channel relocation to the plasma membrane in high-grade DCIS cells using TRPV4-specific pharmacologic inhibitors under normal cell density conditions without crowding. Pharmacologically activating TRPV4 in high-grade DCIS cells under cell crowding conditions resulted in a significant calcium spike, reflecting that the majority (>80%) of TRPV4 was located on the plasma membrane due to crowding. These data suggest that crowding-induced TRPV4 relocation to the plasma membrane via channel inhibition primed the inactive ion channel for activation, thereby counteracting decreased calcium levels. In contrast, activating TRPV4 under cell crowding conditions triggered channel uptake, indicating that TRPV4 trafficking depended on the channel’s activation status.
Remarkably, high-grade DCIS cells exhibited significant cell volume reduction under cell crowding conditions, indicating high cell volume plasticity. In contrast, ADH-mimicking cells showed largely unchanged cell volumes, reflecting low cell volume plasticity and a lack of mechanosensing capability. This high cell volume plasticity in high-grade DCIS cells was further evidenced by the dose-dependent cell volume changes observed with TRPV4 inhibitor and activator treatments, which correlated with the extent of plasma membrane relocation of TRPV4. Cell volume reduction induced by cell crowding or pharmacologic TRPV4 inhibition increased cell invasiveness and motility. This increase strongly correlated with the greater association of TRPV4 with the plasma membrane in high-grade DCIS cells, highlighting a marked selectivity for this cell type.
Further analysis of patient samples indeed confirmed that the selective relationship between plasma membrane TRPV4 association and pro-invasive cell volume reduction pathway was exclusive to high-grade DCIS cells, which was not present in lower-grade DCIS or less aggressive pathologies including ADH. This underscored the selectivity of this mechanotransduction pathway. Additionally, our findings revealed a dichotomy in the pro-invasive mechanosensing capabilities of invasive breast cancer cells, with some lacking these abilities. Notably, this selective pro-invasive mechanotransduction pathway was also activated by hyperosmotic stress, which led to ion channel relocation to the plasma membrane and osmotic cell volume reduction. The convergence of these endpoints resulted from distinct mechanical stimuli highlights the pivotal role of cell volume reduction in facilitating the invasion of high-grade DCIS cells, which are normally categorized as non-invasive but associated with high risk.
Results
Cell Crowding Enhances Invasiveness in High-Grade DCIS Cells
Cell crowding reflects a common condition in tumor microenvironments for ADH and DCIS, arising from aberrant cell proliferation within spatially constrained intraductal spaces. We examined the influence of this prevalent environmental factor on the invasiveness of these cells in vitro. To conduct this investigation, we assembled a panel of cell lines derived from the normal breast epithelial cell line MCF10A, including its H-RAS mutation-driven derivatives associated with various pathologies28: MCF10AT1, MCF10DCIS.com, and invasive cells MCF10CA1a. MCF10AT1 resembles ADH15, MCF10DCIS.com mimics high-grade DCIS26, and MCF10CA1a represents a malignant invasive cancer that was observed to form metastatic lesions in a mouse xenograft29. The current classification of DCIS relies on histological factors such as cell growth patterns and cytonuclear features30,31. Comedo-DCIS is a histologic subtype, which is characterized by apoptotic cell death, representing a high-grade DCIS with higher invasive potential than those of lower-grades25. MCF10DCIS.com forms comedo-type DCIS lesions that can spontaneously transition to invasive cancer when xenografted26.
To compare cell invasiveness under normal cell density and cell crowding conditions in vitro, we opted for a modified 2D matrix degradation assay. This approach allowed us to quantify overall cell invasiveness by normalizing it with the total cell number, thereby accounting for differences in cell densities. We chose this method over transwell, 3D Matrigel, or spheroid assays, where quantifying cell invasiveness as a function of cell density is challenging. By modifying an existing collagen-crosslinked polyacrylamide hydrogel matrix-based invasion assay32–34, we could determine the invasive cell fraction out of the total cell population by fluorescence imaging of the invasion gel bed and cell nuclei through a low magnification (4X) imaging (Fig. 1A; for a detailed procedure, see Suppl. Fig. 1) when cells were at normal density versus under cell crowding conditions.
We observed alterations in live cell morphology and invasiveness as they progressed from normal density (ND: 40-70%) to full confluence (100%) and beyond. Our goal was to determine a distinct time window, termed overconfluence (OC), where cell crowding-induced changes level off. The OC time window was achieved as cells cultured for an additional 5-10 days after reaching 100% confluence, with the growth medium replaced twice per day to prevent acidification. The schematic of the timeline to achieve the OC conditions, and low-magnification (4X) bright-field images at different time points from the ND to OC conditions are shown in Suppl. Fig. 2A-B. The time-dependent equilibration of cell invasiveness after the cells reached confluence is demonstrated in Suppl. Fig. 2C-D using our quantifiable collagen-crosslinked polyacrylamide hydrogel matrix-based invasion assay. Cell crowding mechanosensing significantly increased the invasive cell fraction of MCF10DCIS.comcells from ∼24% (ND cells) to 59% (OC cells). In contrast, other cell types such as normal breast epithelial cells MCF10A and MCF10AT1, which mimic ADH conditions in vivo, did not display invasiveness in ND conditions or enhanced invasiveness in OC conditions, suggesting insensitivity to cell crowding (Fig. 1B-C).
The invasive cell fraction of ND MCF10CA1a cells was already ∼80%, and the additional increase in invasiveness due to cell crowding was not readily discernible, with a slight increase to ∼82% under crowded conditions. These data suggest a striking and selective mechanosensing effect of cell crowding on cell invasiveness in MCF10DCIS.comcells. To ensure that acidification did not affect the invasiveness of MCF10DCIS.com cells despite the frequent replacement of the cell growth medium, we incubated ND cells for two days with acidified medium from cultures of OC MCF10DCIS.comcells. We observed that medium acidity did not alter cell invasiveness (Suppl. Fig. 2E), reinforcing that the increased invasiveness under OC conditions was induced by cell crowding.
Cell crowding reduces cell volume and stiffens MCF10DCIS.comcells
As cells became crowded, we observed a reduction in cell size. Previous reports indicate an inverse relationship between cell volume and cortical stiffness35,36, leading us to hypothesize that reduced cell volume would be accompanied by increased cortical stiffness. Research on glioma cell invasion underscores the critical role of hydrodynamic cell volume changes in penetration into surroundings37,38, suggesting that significant cell volume reduction facilitates cell invasion. Additionally, increased cortical stiffness is known to help cells overcome the physical barriers of the dense extracellular matrix39,40. We thus investigated whether cell crowding selectively induces pro-invasive cell volume reduction and cell stiffening in MCF10DCIS.comcells, thereby priming them for invasion.
To measure the cell volume of individual cells, we used confocal microscopy with a 60X oil immersion objective to obtain z-stack images of live or fixed cells stably expressing red fluorescent protein (RFP)41. Using a nanoindenter attached to the confocal microscope (Suppl. Fig. 3A), we extracted Young’s modulus of live individual cells to assess changes in cortical stiffness. Cell crowding significantly reduced cell volume (Fig. 2A, B) and increased cortical stiffness (Fig. 2C) in both MCF10DCIS.comand MCF10A cells. However, the changes in volume and stiffness between ND and OC conditions were more pronounced in MCF10DCIS.comcells (Suppl. Fig. 3B, C). The cell crowding-induced alterations in cell volume and stiffness were negligible in MCF10AT1 cells (Fig. 2A-C). In MCF10CA1a cells, cell crowding led to an increase in stiffness, but a decrease in volume was not evident. This was expected because the cell volume was already too small to be accurately measured with the available resolution (Fig. 2A-C). The notable plasticity in cell volume and stiffness changes observed in MCF10DCIS.comcells in response to cell crowding potentially underscores the critical link between this plasticity and mechanosensitive increases in cell invasiveness. We assessed cell volume changes only as an effector event of cell crowding, without measuring cell stiffness, because cell volume reflects the mechanical properties of the entire cell, while Young’s modulus can vary depending on the location of indentations in the plasma membrane42,43. The observed cell volume changes by cell crowding (Fig. 2A) showed the same trend in the ND cell volume, with final volumes under crowded conditions being comparable across all cells. Consequently, the ND cell volume and the volume changes from ND to OC conditions exhibited an approximately linear relationship, with an R² ∼ 0.97 (Suppl. Fig. 3D), indicating a highly linear correlation. This finding suggests that the cell volume at ND could serve as an indicator of relative cell volume plasticity.
To validate the pro-invasive nature of cell volume reduction in MCF10DCIS.comcells, we examined the effect of hyperosmotic cell volume reduction in ND cells using polyethylene glycol (PEG) 300 for 15 minutes. The cell volume reduction effects of this treatment were previously described36,44. PEG 300 effectively reduced ND MCF10DCIS.com cell volume in a dose-dependent manner, showing a greater reduction with 148.8 mOsmol/kg PEG 300 than with 74.4 mOsmol/kg PEG 300 (Fig. 2D). To assess the impact of PEG-induced cell volume reduction on cell invasiveness, we used collagen-crosslinked polyacrylamide hydrogel matrix-based invasion assay and exposed cells to 74.4 mOsmol/kg PEG 300 for 2 days. While treatment with 148.8 mOsmol/kg PEG 300 significantly reduced cell viability, cells treated with 74.4 mOsmol/kg PEG 300 remained viable for 2 days. Monitoring changes in cell invasiveness revealed that exposure of ND MCF10DCIS.com cells to 74.4 mOsmol/kg PEG 300 increased cell invasiveness (Fig. 2E), confirming the causal relationship between cell volume reduction and increased cell invasiveness.
Cell crowding induces TRPV4 relocation to plasma membrane in MCF10DCIS.com cells
Cell volume regulation typically depends on osmotic gradients that direct the movement of water across cell membranes45. This process is facilitated by ion flux modulation, which is controlled by ion channels and ion transporters located on the plasma membrane46–49. Given the observation that cells capable of attaining minimal cell volume can successfully penetrate neighboring tissues38, we speculated that the high cell volume plasticity observed in MCF10DCIS.com cells reflects this ability. For such efficient cell volume changes, we hypothesized that cell crowding mechanosensing in MCF10DCIS.com cells would alter the number of plasma membrane-associated ion channels or transporters.
To compare the plasma membrane associated proteins between ND and OC conditions, we employed mass spectrometry and profiled those proteins using the streptavidin-pulled surface-biotinylated cell lysates. Fig. 3A depicts the relative protein densities on the plasma membrane between the ND (blue bars) and OC (red bars) conditions for the top 25 proteins showing over 5-fold increases (triangle plots) in protein expression on the plasma membrane in the OC condition compared to the ND condition in MCF10DCIS.com cells. The gene names of these 25 proteins are presented in Fig. 3A (Suppl. Table 1 provides the corresponding protein names along with the corresponding fold increases). Notably, TRPV4, a member of the transient receptor potential family of ion channels known for its mechanosensitive properties50,51, showed a remarkable 153-fold increase in plasma membrane association in response to cell crowding, as illustrated in Fig. 3A. Additionally, we observed an increase in plasma membrane association of other ion channels, such as SCN11A (the alpha subunit of the voltage-gated sodium channel Nav1.9) with ∼42-fold enrichment, and KCNN4 (the small-conductance calcium-activated potassium channel 3 SK3) showing ∼6-fold increase, as shown in Fig. 3A. To evaluate the relative plasma membrane association of proteins under OC versus ND conditions, we performed a comparable mass spectrometry analysis of cell-surface biotinylated cell lysates for MCF10A, MCF10AT1, and MCF10CA1a cells. The gene/protein names showing increased plasma membrane associations in these cells are listed in Suppl. Table 2. Unlike in MCF10DCIS.com cases, there was no cell-crowding-induced relocation of ion channels and ion transporters to the plasma membrane in MCF10A and MCF10AT1 cells. However, in MCF10CA1a cells, ion transporters such as ATP2B4 showed a ∼37-fold greater plasma membrane association under crowding conditions. This suggests that plasma membrane relocations of these ion channels and ion transporters in response to cell crowding selectively occurred in MCF10DCIS.com cells and, to an extent, in invasive ductal cancer MCF10CA1a cells.
TRPV4 plays a pivotal role in facilitating the passage of calcium ions (Ca2+) and is integral in detecting various forms of mechanical stresses, including temperature fluctuations and osmotic pressure52,53. Activation of TRPV4 channels elevates intracellular calcium levels, leading to cell volume increase via osmotic water influx52,54. Nav1.9 primarily contributes to the generation and propagation of action potentials in sensory neurons and is associated with various pain disorders55–57. SK3 channels are activated by elevated intracellular calcium levels and serve diverse physiological functions58,59. However, unlike TRPV4, Nav1.9 and SK3 channels are not generally classified as mechanosensors. Consequently, we focused on TRPV4, the mechanosensor channel displaying the most significant increase in the plasma membrane association under cell crowding conditions, to further investigate its role in promoting pro-invasive cell volume reduction in MCF10DCIS.comcells.
To confirm the selective plasma membrane association of TRPV4 in OC MCF10DCIS.comcells suggested by mass spectrometry results, we conducted immunoblots on surface-biotinylated lysates from all four cell types under both conditions. Fig. 3B and Suppl. Fig. 4A showed a notable association of TRPV4 with the plasma membrane in OC MCF10DCIS.comcells, which was absent in ND cells. This association was not observed in MCF10A and MCF10AT1 cells under any conditions. Interestingly, in MCF10CA1a cells, TRPV4 associated with the plasma membrane under both conditions, with a slight increase in OC conditions. This increase suggests that these cells may also possess mechanosensing abilities, enabling them to respond to cell crowding. Additionally, immunoblots from whole-cell lysates showed consistent TRPV4 expression levels across different cell types and densities (Fig. 3C, Suppl. Fig. 4B), indicating that cell crowding influences ion channel trafficking without altering overall expression. This evidence strongly suggests that the relocation of TRPV4 to the plasma membrane is a mechanosensitive response to cell crowding.
To investigate the redistribution of TRPV4 in response to cell crowding, we performed IF imaging with confocal microscopy across all four cell types under both ND and OC conditions. The binding specificity of the TRPV4 antibody was confirmed through IF imaging (Suppl. Fig. 5A) and immunoblotting (Suppl. Fig. 5B) of MCF10DCIS.comcells treated with TRPV4 shRNA. As anticipated from the mass spectrometry results, TRPV4 (red) was significantly associated with the plasma membrane, selectively observed in OC MCF10DCIS.comcells in the IF images shown in Fig. 3D. In MCF10CA1a cells, TRPV4 was already associated with plasma membrane under ND conditions, but cell crowding increased the association significantly. Plasma membrane associations were verified using the plasma membrane marker DiIC18(3), which we previously used to visualize the plasma membrane60. IF images showed that TRPV4 successfully colocalized with DiIC18(3) in OC MCF10DCIS.comand OC MCF10CA1a cells (Suppl. Fig. 6A). However, the non-interacting protein transferrin receptor (TfR; green) remained similarly distributed in the OC cells compared to the ND cells, indicating that the protein trafficking changes induced by cell crowding are specific to TRPV4 (Fig. 3D).
To investigate whether other ion channels identified through mass spectrometry and a well-known mechanosensory channel PIEZO161,62, exhibit similar behavior, we conducted IF imaging on ND and OC MCF10DCIS.comcells targeting KCNN4 and PIEZO1. Unfortunately, due to the absence of specific antibodies, we were unable to examine SCN11. In ND MCF10DCIS.comcells, KCNN4 was observed to be mostly cytosolic, while PIEZO1 was mildly but slightly more associated with the plasma membrane than TRPV4 under ND conditions. However, under OC conditions, both PIEZO1 and KCNN4 also relocated to the plasma membrane, albeit to a lesser extent than TRPV4 (Suppl. Fig. 6B). The varying degrees of plasma membrane association of PIEZO1 and KCNN4 are quantified using line analysis (Supple. Fig. 6C).
As expected, in ND MCF10A, MCF10AT1, and MCF10DCIS.comcells, TRPV4 was predominantly intracellular, with notable enrichment in the nuclei (Fig. 3D). However, in OC MCF10DCIS.comcells, approximately 80% of TRPV4 was located in the plasma membrane based on line analysis (Fig. 3E). In contrast, in OC MCF10A and OC MCF10AT1 cells, TRPV4 remained cytosolic but migrated out of the nuclei (Fig. 3D). TRPV4 association with the plasma membrane was observed in both ND and OC MCF10CA1a cells, with an observable increase under OC conditions, further confirming the immunoprecipitation results shown in Fig. 3B. Plasma membrane-associated TRPV4 was quantified using line analysis and plotted in Fig. 3E. Refer to Suppl. Fig. 7A for plots of the relative TRPV4 associations with the plasma membrane, cytosol, and nucleus between ND and OC conditions in all four cell types. These IF imaging results underscore the mechanosensitive plasma membrane relocation of ion channels in MCF10DCIS.co, and to an extent, in MCF10CA1a cells, contrasting with the observed insensitivity of MCF10A and MCF10AT1 cells to cell crowding.
To assess the relationship between cell crowding-induced cell volume reduction and plasma membrane relocation of TRPV4, we investigated whether hyperosmotic conditions, which reduce cell volume without cell crowding, would also result in TRPV4 relocation. We subjected ND cells to the same PEG 300 condition (74.4 mOsm/kg PEG for 15 minutes) used in Fig. 2D to induce cell volume reduction. In ND MCF10DCIS.comcells, such hyperosmotic cell volume reduction prompted plasma membrane relocation of TRPV4, a response not observed in MCF10A and MCF10AT1 cells, but seen in MCF10CA1a cells (Fig. 3F). Quantitative line analysis confirmed these findings by assessing the relative TRPV4 association with the plasma membrane (Fig. 3G; Suppl. Fig. 7B for plots of the relative TRPV4 association with the plasma membrane, cytosol, or nucleus). This finding underscores the relationship between mechanosensitive cell volume reduction and plasma membrane relocation of TRPV4, highlighting that both cell crowding and hyperosmotic stress lead to the same effect, particularly pronounced in MCF10DCIS.comcells.
Patient tissue analysis shows selective plasma membrane association of TRPV4 in high-grade DCIS cells
MCF10DCIS.comcells represent a basal cell model for high-grade DCIS cells driven by HRAS mutation26,63. However, the majority of patient-derived DCIS cells originate from the luminal cell population and lack the HRAS mutation63. Moreover, there are limited options for patient-derived DCIS cell line models that have not linked to well-defined pathology64,65. Thus, to seek generality of our finding about the selective association between plasma membrane relocation and high-grade DCIS pathology, we examined 39 breast cancer patient tissue blocks to assess the selectivity in vivo. We designed an early-stage retrospective study by selecting various breast tissue pathologies that range from benign to invasive cancer. Those pathologies include usual ductal hyperplasia, papilloma, columnar changes, ADH, low-to high-grade DCIS, and invasive ductal carcinoma (IDC). We also incorporated normal regions for comparison. Hematoxylin and eosin (H&E) staining was used to visualize tissue sections from each patient, excluding samples with prior cancer diagnoses or drug treatments. In total, 97 regions of interest (ROIs) from H&E-stained sections of 39 patient tissue blocks were selected. Two pathologists independently assessed TRPV4 distribution patterns at the single-cell level in corresponding ROIs in the immunohistochemistry (IHC) images (Fig. 3H). A detailed methodology is described in the Methods section and more example images are shown in Suppl. Fig. 8. High-grade DCIS cells, as depicted in Fig. 3H, clearly demonstrated plasma membrane-associated TRPV4, a feature absent in lower-grade DCIS cells (intermediate- and low-grade). This distinction sets them apart not only from cells with ADH and benign pathologies but also from lower-grade DCIS cells. As demonstrated by the IF images in Fig. 3D for MCF10CA1a (invasive cell mimic), IDC cells exhibited notable plasma membrane TRPV4, suggesting that they possess a similar pro-invasive mechanotransduction capability to high-grade DCIS cells.
We found that the sensitivity and specificity for plasma membrane TRPV4 in high-grade DCIS cells were 0.75 ± 0.19 (15/20) and 0.98 ± 0.03 (61/62) respectively (95% confidence interval) (Fig. 3I). It is important to note that the specificity calculation excluded IDC cases. Notably, even in less aggressive pathologies, a significant amount of TRPV4 was localized in the nuclei, as shown for MCF10A and MCF10AT1 cells in the IF images in Fig. 3D. This underscores our interpretation from the in vitro results in Fig. 3A-D that increased plasma membrane association of TRPV4 in high-grade DCIS cells results from changes in protein localization through trafficking alterations, rather than differences in expression levels. These initial in vivo results clearly demonstrate the selective and specific association of TRPV4 with the plasma membrane in high-grade DCIS cells. Notably, this association is absent in lower-grade DCIS, ADH, benign, and normal cells, thereby confirming our in vitro findings using MCF10A cell derivatives. These results suggest a potentially critical role for TRPV4 in the progression of high-grade DCIS.
Cell crowding inhibits ion channels and triggers their plasma membrane relocations
A calcium-permeant ion channel like TRPV4 is known to influence intracellular calcium dynamics54. While activation of TRPV4 typically elevates calcium levels and this potentially increases cell volume66–68, the impact of its inhibition is less clear, given the multifaceted nature of calcium signaling in cell volume control69. This complexity is compounded by compensatory cellular mechanisms and the involvement of other ion channels in response to altered calcium homeostasis46. Considering these factors, we hypothesized that cell crowding might inhibit calcium-permeant ion channels, including TRPV4, which would then lower intracellular calcium levels and subsequently reduce cell volume via osmotic water movement. To test this, we employed the Fluo-4 calcium assay70 to compare relative intracellular calcium levels of MCF10DCIS.comcells between ND and confluent (Con) conditions through 4X confocal microscopy imaging with 488 nm excitation. For this calcium assay, we opted for confluent conditions instead of OC conditions to collect the Fluo-4 signal from confluent monolayers of MCF10DCIS cells, which demonstrates comparable cell-crowding induced TRPV4 relocation to the plasma membrane (Suppl. Fig. 9A) as observed in the OC condition. This choice was made because more than one layer of cells was occasionally observed in OC conditions, which would yield overestimation of crowding-dependent intracellular calcium levels. The intracellular Fluo-4 signal under Con conditions was significantly lower than that under ND conditions, as shown in the fluorescence images of ND versus Con cells in Fig. 4A. Time-dependent Fluo-4 images were acquired over 25 minutes. For ND cell images, the average Fluo-4 intensity values were calculated from 10-15 selected cells (highlighted in the white box in Fig. 4A). For Con cell images, intensity values were derived from all cells within the entire 50 μm by 50 μm field of view, as depicted in Fig. 4A. After background subtraction, the intensity values were plotted in Fig. 4B. The temporal Fluo-4 intensity profiles from both ND and Con cells remained largely constant over the measured 20-minute period. However, Con cells exhibited significantly lower Fluo-4 intensity compared to ND cells, indicating reduced intracellular calcium levels. Lower Fluo-4 intensity in Con cells than in ND cells was not due to limited Fluo-4 reagent in Con samples. Within cell clusters, cells experiencing crowding exhibited notably lower Fluo-4 levels compared to those at the periphery (Suppl. Fig. 9B), suggesting that cell crowding had resulted in a decrease in intracellular calcium levels, likely due to the mechanosensitive inhibition of calcium-permeable channels like TRPV4.
To investigate whether TRPV4 inhibition was involved in decreased intracellular calcium levels and subsequent relocation of TRPV4 to the plasma membrane, we employed TRPV4-specific pharmacological agents to alter TRPV4 activity and modulate calcium concentrations in ND MCF10DCIS.comcells without applying cell crowding conditions. To inhibit TRPV4 activity, we used TRPV4 inhibitor (GSK2193874; GSK219)71 at 0.2 and 1 nM, which we confirmed to have insignificant effects on cell viability in both ND and OC conditions for two days (Suppl. Fig. 10A). This allowed us to use these conditions for the invasion assays described below.
GSK219 treatments immediately reduced the Fluo-4 signal, followed by a recovery, indicating cellular homeostatic activity. Fig. 4D shows Fluo-4 images before and at the signal dip after applying 1 nM GSK219. Fig. 4E presents the temporal profile of Fluo-4 intensity, marking two time points: t1 (baseline) and t2 (dip). Although the Fluo-4 dip at t2 was not visibly apparent in the image of ND MCF10DCIS.comcells (Fig. 4D), the temporal profile (Fig. 4E) clearly showed TRPV4 inhibition by GSK219. The extent of inhibition was reflected in the calcium depletion, ΔCa (vertical dashed line between the dip and baseline), followed by homeostatic recovery.
In Con cells, 1 nM GSK219 led to a very small dip in Fluo-4 intensity with no changes afterward, likely due to the already low intracellular calcium levels caused by cell crowding. Fig. 4F shows the baseline and dip Fluo-4 images, while Fig. 4G presents the temporal Fluo-4 intensity profile, highlighting the two time points. The reduction in Fluo-4 intensity induced by GSK219 was smaller in Con MCF10DCIS.comcells (mean ΔCa ∼ −39 ± 9) compared to ND cells (mean ΔCa ∼ −49 ± 18), as illustrated in Fig. 4H. This smaller reduction in calcium in Con cells may also be due to less active TRPV4 channels, resulting from cell crowding-induced TRPV4 inhibition. The dose-dependent (0.2 nM and 1 nM GSK219, labeled 219L and 219H) and cell density-dependent (ND and Con) Fluo-4 signal reductions were reflected in the ΔCa values plotted in Fig. 4H. The results show the most significant TRPV4 inhibition in ND MCF10DCIS.comcells at 1 nM GSK219. These data suggest that a significant portion of TRPV4 that relocated to the plasma membrane in response to cell crowding was in an inactive state.
Administering a TRPV4 activator (GSK1016790A; GSK101)72,73 immediately caused a notably greater spike in Fluo-4 signal in Con cells than in ND cells, evidencing that a large number of inactive TRPV4 channels were indeed associated with the plasma membrane in Con cells. In Con cells, 0.2 pM GSK101 led to a Fluo-4 signal spike with ΔCa ∼ +2,348 ± 597, while in ND cells, ΔCa was ∼ +324 ± 130. This difference is clearly depicted in the Fluo-4 images (Fig. 4I for ND and Fig. 4K for Con) and the time-dependent Fluo-4 intensity plots (Fig. 4J for ND and Fig. 4L for Con). The dose (101L and 101H representing 0.05 pM and 0.2 pM GSK101) and cell-density (ND and Con) dependent Fluo-4 signal spikes are reflected in the ΔCa values, as shown in Fig. 4M. The results show the most significant TRPV4 activation in Con MCF10DCIS.comcells at 0.2 pM GSK101, confirming a large number of primed inactive TRPV4 channels at the plasma membrane in Con cell.
Based on the differing effects of GSK219 and GSK101 on immediate calcium responses, we hypothesized that cell crowding induced TRPV4 inhibition and subsequent calcium depletion, which triggered the plasma membrane relocation of TRPV4 to compensate for the loss of calcium. We examined GSK219 and GSK101 dose-dependent relocation in both ND and OC MCF10DCIS.comcells via IF imaging (Fig. 4N for higher GSK101/219 concentrations and Suppl. Fig. 10B for both concentrations). Treating ND cells with GSK219 for 1 hour confirmed plasma membrane relocation of TRPV4 (∼53 ± 7%) at 0.2 nM and more (∼62 ± 7%) at 1 nM, significantly higher than in control ND cells (∼15 ± 6%), as shown by the line analysis (Fig. 4P). These data indicate that TRPV4 inhibition triggered its strategic relocation to the plasma membrane, preparing it for rapid activation to offset the channel inhibition effects.
Following the trend of GSK101-induced small Fluo-4 spikes in ND MCF10DCIS.comcells (Fig. 4J), the plasma membrane associated TRPV4 remained similarly with GSK101 to the control condition (Fig. 4N, 4P). However, in Con cells, GSK101 diminished the plasma membrane associated TRPV4 channels in a dose-dependent manner through internalization (Fig. 4O, 4Q), consistent with previous findings65. These data suggest that plasma membrane TRPV4 levels were largely regulated by the channel activity status. Specifically, channel activation led to the internalization of TRPV4, while channel inhibition promoted the relocation of TRPV4 to the plasma membrane. However, we observed that GSK219 treatment in Con cells results in a slight reduction of TRPV4 from the plasma membrane, rather than an expected increase via additional plasma membrane relocation (Fig. 4O, 4Q). Considering that nuclear TRPV4 levels increase with higher doses of GSK219 in Con cells (Suppl. Fig. 10B), as opposed to the cytoplasmic TRPV4 levels, we postulate that a distinct trafficking alteration occurs when TRPV4 is further inhibited under crowding conditions, where the channels are largely inactive.
Given the role of TRPV4 in osmoregulation53,74 and its significant relocation to the plasma membrane under hyperosmotic conditions (∼55% plasma membrane association by line analysis; Fig. 3E, 4P) in ND MCF10DCIS.comcells, we explored the potential involvement of TRPV4 inhibition during this relocation. Using the Fluo-4 assay, we observed an initial calcium spike following 74.4 mOsmol/kg PEG 300 in ND MCF10DCIS.comcells due to osmotic water outflow, which was followed by a homeostatic relaxation aimed at restoring calcium levels (Suppl. Fig. 11A). This relaxation likely involved the inhibition of ion channels like TRPV4 and other ion transporters, followed by their relocations to the plasma membrane. Indeed, we confirmed the relocation of other ion channels that responded to cell crowding, including KCNN4 and PIEZO1, to the plasma membrane following the same PEG 300 treatment (Suppl. Fig. 11B for IF images and line analysis results). The data suggest that the increased association of TRPV4 with the plasma membrane under hyperosmotic stress may reflect an adaptive inhibition response to water outflow.
Cell crowding-induced inactive TRPV4 relocation to the plasma membrane indicates inhibition-mediated volume reduction and enhanced invasiveness and motility
We next investigated whether the inhibition-driven plasma membrane relocation of inactive TRPV4 precedes the reduction in cell volume and the enhancement of cell invasiveness observed in response to cell crowding (as shown in Fig. 2A, 2B). To explore these relationships, we examined the effects of 2-day treatments with GSK101 and GSK219 on the cell volumes of ND and OC MCF10DCIS.comcells. This duration was selected to correlate the treatments with changes in cell volume and invasiveness.
Under ND conditions, activating TRPV4 with GSK101 (0.05 or 0.2 pM for 2 days), which caused minor calcium spikes and left TRPV4 distribution largely unaltered (Fig. 3A; Suppl. Fig. 6A), did not significantly alter cell volume (Fig. 5A). Conversely, TRPV4 inhibition by GSK219 treatment (1 nM for 2 days) that induced greater calcium changes (dips rather than spikes) and a significant plasma membrane relocation of TRPV4, led to a noticeable cell volume reduction (Fig. 5A). 0.2 nM GSK219 treatment had a negligible impact on ND cell volume (Fig. 5A). The GSK219 effect in ND MCF10DCIS.comcells was similar to that observed under hyperosmotic conditions by 74.7 mOsm/Kg PEG 300 (Fig. 5B). In contrast, under OC conditions, GSK101, which triggered dose-dependent calcium spikes, led to corresponding increases in cell volume. In contrast, GSK219, which had a minimal impact on intracellular calcium levels and did not significantly alter TRPV4 distribution at the plasma membrane, resulted in no significant change in cell volume. The effect of GSK219 on cell volume reduction in OC conditions was similar to that of PEG 300 (Fig. 5B). As expected, a significant linear correlation was observed between the plasma membrane association of TRPV4 and changes in cell volume (R² ∼ 0.59) (Fig. 5C). This finding confirms that the channel inhibition-driven relocation of TRPV4 to the plasma membrane in response to cell crowding, and the subsequent decrease in intracellular calcium, are associated with a reduction in cell volume in MCF10DCIS.comcells.
Next, we examined the overall connections between TRPV4 activity, its plasma membrane association, cell volume changes, and cell invasiveness. Thus, we evaluated changes in invasiveness following 2-day treatments with GSK101 or GSK219 using collagen-crosslinked polyacrylamide hydrogel matrix-based invasion assay used in Fig. 1. In ND MCF10DCIS.comcells, 0.2 pM GSK101 led to only a mild suppression of cell invasiveness, and 0.05 pM GSK101 did not alter cell invasiveness (Fig. 5D). In contrast, under ND conditions, GSK219 caused a dose-dependent increase in the invasive cell fraction, mirroring the patterns for Fluo-4 signal changes and plasma membrane-associated TRPV4 (Fig. 5D). Under OC conditions, GSK101 increased cell volume and suppressed cell invasiveness in a dose-dependent manner (Fig. 5E). Conversely, both GSK219 and PEG 300, which reduced cell volume, led to increased cell invasiveness (Fig. 5E). The treatment dependent invasion results are shown in Suppl. Fig. 12A. This solidified the relationship between TRPV4 activity, plasma membrane-associated TRPV4, cell volume, and cell invasiveness. There were significant linear correlations between plasma membrane-associated TRPV4 and cell invasiveness changes (Fig. 5F; R² ∼ 0.69), and between cell volume and cell invasiveness changes (Fig. 5G; R² ∼ 0.89).
Cell invasiveness refers to the ability of cells to penetrate and move within their surrounding tissues or environments. As expected from the increased cortical stiffness of OC MCF10DCIS.comcells (Fig. 2C), we observed that OC MCF10DCIS.comcells underwent cytoskeletal rearrangements, including the formation of stress fibers (Suppl. Fig. 12B), which likely enhanced cell motility75. To assess how TRPV4 inhibition and activation, which reduced and increased cell volume, respectively, affected the motility of ND MCF10DCIS.comcells and the role of these motility changes on cell invasiveness, we conducted single-cell tracking experiments similar to single molecule tracking methods60,76–80. We utilized wheat germ agglutinin (WGA) as a nuclear marker, which stained the cell nucleus within 1 hour of incubation with cells. We then analyzed the diffusivity (D) and directionality (v) of single cell movements. We tracked overall single-cell trajectories for 3 hr with 1 min intervals, marking cell displacement over each interval in different colors, ranging from navy for the slowest to red for the fastest (Fig. 5H). Consistent with the trend in cell invasiveness, TRPV4 activation with 1 pM GSK101 significantly reduced single-cell movements, while inhibition with 0.2 nM GSK219 and 74.4 mOsm/kg PEG 300 notably increased these movements. Interestingly, while GSK219 and hyperosmotic conditions promoted increased cell movement (D; Fig. 5I), they also reduced cell directionality (v; Fig. 5I). This reduction in directionality suggests that while the cells become more mobile, their movements are less targeted, potentially indicating a more random or exploratory behavior under mechanical stress. Remarkably, cell movement increased in proportion to the amount of TRPV4 at the plasma membrane (Fig. 5K; R² ∼ 0.73) and decreased with increased cell volume (Fig. 5L; R² ∼ 0.89). Cell movement and invasiveness showed a strong linear correlation (Fig. 5M; R² ∼ 0.85), suggesting that these behaviors are governed by the same underlying mechanisms.
Mechanosensitive TRPV4 relocation to plasma membrane indicates enhanced invasiveness
We investigated whether the mechanosensitive relocation of TRPV4 to the plasma membrane can reliably predict a cell’s ability to undergo pro-invasive mechanosensitive cell volume reduction. To achieve this, we used changes in cell motility as markers for overall shifts in cell invasiveness, given the strong correlation observed between cell motility and invasiveness (Fig. 5M). Our hypothesis is that cells with such mechanotransduction capability, like MCF10DCIS.comcells, should show increased plasma membrane association of TRPV4 when inhibited with GSK219 or under hyperosmotic conditions, evidenced by increased cell motility (D) while demonstrating the opposite effect with GSK101 treatment.
For comparison, we employed three uncharacterized cell types: MDA-MB-231, a triple-negative invasive breast cancer cell line, and two recently developed patient-derived DCIS cell lines, ETCC-06 and ETCC-1064,65, which have not yet been histologically classified by grade. These cells were compared with positive control groups, including MCF10DCIS.comand MCF10CA1a, which were observed to possess pro-invasive mechanotransduction capability (Fig. 5D, 6A-6C). MCF10AT1 served as a negative control, as it lacks this capability (Fig. 6D-6F).
MCF10CA1a cells showed plasma membrane relocation of TRPV4 in response to TRPV4 inhibition (1 nM GSK219; ND 219H), PEG 300 (74.4 mOsm/kg; ND PEG300), and OC conditions. In contrast, treatment with 0.2 pM GSK101 did not affect TRPV4 plasma membrane association (Supplementary Fig. 13 and Fig. 6A, showing IF images for TRPV4 (red) and DAPI (cyan)). This response was similar to that observed in MCF10DCIS.comcells under OC conditions. The plasma membrane associations of TRPV4 were quantified by line analysis, showing an increase under mechanical stresses (PEG and OC) and TRPV4 inhibition with GSK219, as plotted in Fig. 6B. As expected, based on the differences in plasma membrane TRPV4 association, MCF10CA1a cells exhibited increased motility (D) in response to TRPV4 inhibition (GSK219) and hyperosmotic conditions (PEG). Additionally, their movement became more directional (v) with TRPV4 activation (GSK101), similar to the observations in MCF10DCIS.comcells (Fig. 5I, 5J).
In contrast, the negative control MCF10AT1 cells did not exhibit mechanical stress and TRPV4 activity-dependent plasma membrane relocation of TRPV4, as shown by IF images (Suppl. Fig. 13, Fig. 6D) and line analysis results (Fig. 6E). In these cells, TRPV4 inhibition (GSK219) decreased diffusivity (D), while PEG 300 had no effect. Moreover, movement directionality (v) remained unchanged under all treatment conditions (Fig. 6F).
The three test group cells, MDA-MB-231 (Fig. 6G, 6H), ETCC-06 (Fig. 6J, 6K), and ETCC-10 (Fig. 6M, 6N), failed to relocate TRPV4 to the plasma membrane in response to mechanical stresses (PEG and OC), as shown by IF images and quantification. This suggests that these cells lack the pro-invasive mechanosensitive cell volume reduction capability observed in MCF10DCIS.comcells. Consistently, none of these cells showed increased motility (D) under hyperosmotic conditions or TRPV4 inhibition (Fig. 6I, 6L, 6O).
We plotted these results to show cell diffusivity (D) scaling with varying plasma membrane associations of TRPV4, where MCF10DCIS.com(green hexagon) and MCF10CA1a (red hexagon) exhibited a positive scaling (Fig. 5P). However, the changes in plasma membrane TRPV4 occurred over a narrower range (31 – 79%) for MCF10CA1a compared to MCF10DCIS.comcells (16 - 75%), reflecting the larger mechanosensitive cell volume plasticity of MCF10DCIS.comcells. In contrast, MDA-MB-231, ETCC-6, and ETCC-10 cells did not show this scaling (Fig. 5Q). Like MCF10AT1 cells, plasma membrane TRPV4 associations in these cells remained at similar percentages (Fig. 5Q).
This dichotomy in the presence of a pro-invasive mechanotransduction program is evident in the plots of diffusivity (D) increase versus plasma membrane TRPV4 relocation (Fig. 5R), where only MCF10CA1a and MCF10DCIS.comcells show a significant increase in both parameters. Furthermore, the relationship between plasma membrane-associated TRPV4 and D increase with GSK219 versus GSK101 can be used to assess the presence of a pro-invasive, mechanosensitive cell volume reduction program. Most non-mechanotransducing cells remained close to the baseline value of 1, while mechanotransducing cells showed a significant increase in D with GSK219 treatment.
Discussion
Our study explored how cell crowding, a common condition in diseases and development, impacts cell invasiveness. We employed novel assays to quantify cell invasion and single-cell motility in cells with breast tissue pathologies, including DCIS and ADH, which experience cell crowding due to abnormal proliferation in confined ducts. Our results revealed a novel mechanotransduction pathway activated by crowding in high-grade DCIS cells, establishing a direct link between crowding and invasiveness. This pathway highlights the intricate interplay between mechanical stimuli, ion channel activation, trafficking changes, cell volume reduction, stiffening, and invasiveness (Fig. 7). It distinguishes high-grade DCIS cells from normal, hyperplastic, or lower-grade DCIS cells, supporting previous findings that minimal cell volume reduction enables efficient invasion38.
The correlation between cell volume reduction and increased invasiveness highlights the specialized mechanotransduction abilities of high-grade DCIS cells. Using mass spectrometry, we identified TRPV4 as a crucial component of this pathway, providing insights into how non-invasive breast cancer cells may evolve into invasive phenotypes. By employing Fluo-4 assays and TRPV4 activators and inhibitors, we found that cell crowding triggered TRPV4 inhibition, leading to reduced intracellular calcium and subsequent cell volume reduction. Notably, TRPV4 inhibition prompted its relocation to the plasma membrane, priming it for activation under suitable signaling conditions, thereby maintaining cellular homeostasis. In high-grade DCIS cells, TRPV4 inhibition by cell crowding increased invasiveness and motility, while TRPV4 activation had the opposite effect. This phenomenon differed from other invasive breast cancer cell lines, where TRPV4 inhibition typically suppresses invasiveness, and activation enhances it. However, the modest mechanotransduction capability observed in MCF10CA1a cells suggests heterogeneity in invasive cancer progression.81–83.
Our discovery of the selective mechanotransduction pathway in high-grade DCIS cells was further validated by analyzing patient-derived breast cancer tissues. We found that TRPV4 was predominantly associated with the plasma membrane in high-grade DCIS lesions, but not in lower-grade DCIS or less aggressive pathologies. This selective relocation reinforces TRPV4’s crucial role in the pro-invasive mechanotransduction pathway unique to high-grade DCIS cells. The association of TRPV4 with the plasma membrane may serve as a valuable biomarker for identifying high-grade DCIS lesions.
Our data indicate that ion channels beyond TRPV4 may contribute to pro-invasive mechanotransduction. Notably, SCN11A and KCNN4 showed increased plasma membrane association under cell crowding conditions, suggesting their roles in modulating ion flux and cell volume and thus contributing to the mechanotransduction response. Although Piezo1 is known to respond to mechanical stimuli62, its lesser plasma membrane relocation compared to TRPV4 under cell crowding and hyperosmotic conditions suggests a lesser role in promoting invasiveness in this case. Further comparisons are needed to determine the dominant ion channel driving mechanotransduction in high-grade DCIS cells. However, it is evident that cell crowding-induced pro-invasive cell volume reduction pathway involves the coordinated action of multiple ion channels27,84.
We made the intriguing discovery that hyperosmotic conditions, which reduced’ cell volume through osmotic water outflow, mimic the effects of cell crowding by inducing similar pro-invasive cell volume reduction. Treatment with hyperosmotic agents like PEG 300 led to TRPV4 relocation to the plasma membrane and increased cell invasiveness, highlighting TRPV4’s mechanosensitive role in response to external mechanical stimuli. This suggests that the convergence of mechanical stresses, including cell crowding and hyperosmotic stress, is a crucial trigger for pro-invasive cell volume reduction pathways, leading to a unified mechanotransduction signaling cascade.
The study’s insights have far-reaching implications, unlocking new research paths in cancer and biological sciences. By elucidating mechanosensitive responses to cell crowding, we can shed light on tissue development, wound healing, and physiological processes involving cell density changes. The parallels between cell crowding and hyperosmotic conditions in driving pro-invasive behaviors warrant further investigation into the intricate mechanisms of cytoskeleton reorganization, ion channel and transporter relocation, and enhanced cell invasiveness and motility. Moreover, our findings pave the way for developing diagnostic and prognostic strategies that leverage the selective intracellular localization patterns of TRPV4 and other mechanosensitive ion channels, potentially guiding clinical decisions for patients with high-grade DCIS and other cancers where cell crowding plays a crucial role.
Materials and methods
Cell culture and treatments
MCF10A cells were purchased from ATCC, and MCF10AT1, MCF10DCIS.co, and MCF10CA1a cells were procured from the Barbara Ann Karmanos Cancer Institute after establishing material transfer. These cells were authenticated prior to purchase, and cells with a low passage number (< 15) were used for experiments. We confirmed that the cells were mycoplasma-free using PCR analysis. MCF10A and MCF10AT1 cells were maintained in DMEM/F12 supplemented with 5% horse serum, 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 10 μg/mL insulin, and 1% penicillin and streptomycin. MCF10A cells were also supplemented with 100 ng/mL cholera toxin. MCF10DCIS.comand MCF10CA1a cells were cultured in DMEM/F12 supplemented with 5% horse serum and 1% penicillin and streptomycin. cells were cultured in 5% CO2 at 37 °C. For TRPV4 activator and inhibitor, we used GSK 1016790A (GSK101; Tocris 6433) and GSK 2193874 (GSK219; Tocris 5106).
For volume measurements, parental cells were mixed with those expressing RFP at a 9:1 ratio to distinguish individual cells. Cells were treated with 2% (74.4 mOsmol/kg) or 4% PEG-300 (148.8 mOsmol/kg; v/v; Millipore Sigma 8074845000) in culture medium for 15 min, 2% PEG-300 for 48 h, or GSK1016790A (Tocris; 0.05 and 0.2 pM) or GSK2193874 (Tocris; 0.2 and 1 nM) for 48 h.
TRPV4 knockdown by shRNA
Cells in growth medium without penicillin and streptomycin were transfected with the hTRPV4 shRNA plasmid (1 and 2 µg; Santa Cruz sc-61726-SH) and Lipofectamine3000 in opti-MEM. At 6 h after transfection, the medium was replaced, and cells were cultured for 30 h before processing for western blot and immunofluorescence analyses.
Immunofluorescence
The cells were fixed with 4% paraformaldehyde (Fisher Scientific) for 20 min, followed by permeabilization with 0.1% saponin (Fisher Scientific) for 5 min at room temperature (RT). After permeabilization, the cells were washed three times in PBS and blocked in 0.1% saponin + 10% BSA in PBS at RT for 1 h. Cells were incubated with primary antibodies overnight at 4 °C in 0.1% saponin in PBS. The primary antibodies used were TRPV4 (Abcam 39260; 1:500 dilution), TfR (ThermoFisher Scientific 13-6800; 1:500 dilution), Piezo1 (Alomone APC-087; 1:500), and KCNN4 (Alomone ALM-051; 1:500). Cells were washed three times in PBS, incubated with fluorescent-tagged anti-rabbit-Alexa 550 or anti-mouse-Alexa 488 secondary antibodies (Thermo Fisher Scientific) and DAPI for 1 h at RT, and imaged by confocal microscopy. For plasma membrane staining, DiIC18(3) (’1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate; DiIC18(3); Thermo Fisher Scientific, Cat # D282) was diluted in warm PBS to 5 uM. Media was removed from the cells, then the 5 uM DiIC18(3) in PBS was added. The dish was transferred to the incubator (37 C, 5% CO2) for 10 minutes. Following the 10 min incubation, the DiIC18(3) in PBS was removed and the cells were washed 2x with complete cell growth media. The cells were then fixed with 2% PFA in PBS
Confocal imaging and image processing
2D images were acquired using a Yokogawa spinning-disk confocal microscope (Andor Technology) installed in a Nikon Eclipse TE2000 inverted microscope using a 60x/1.49NA Plan Apo objective (Nikon). The samples were illuminated using 430, 488, 561, and 647 nm solid-state lasers (Andor Technology). Images were acquired using an iXon back-illuminated EMCCD camera (Andor Technology). For volume measurements, 3D confocal images were acquired using z-step sizes calculated based on Nyquist conditions. Images were processed using the ImageJ (NIH) or Imaris (Bitplane) software. For live cell imaging, cells were maintained in 5% CO2 at 37 °C in a stage-top incubator (Oko-lab).
Western blotting
ND cells (40–60% cell density) were lysed in SDS sample buffer, and OC cells were lysed in cytoskeleton buffer (10 mM Tris pH 7.4,100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate). SDS sample buffer (Bio-Rad) supplemented with reducing reagent was added to lysates and boiled at 100°C for 10 min. The samples were separated on 4–15% SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked in TBST (TBS + 0.1% Tween-20) containing 5% non-fat milk and then incubated with primary TRPV4 antibody (ThermoFisher Scientific PA541066; 1:1000) and GAPDH (Santa Cruz sc-32233; 1:2000) overnight at 4 °C. Membranes were washed three times in TBST (5 min each) and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit (ThermoFisher Scientific A28177) or anti-mouse (ThermoFisher Scientific A28177) secondary antibodies for 30 min at RT. Blots were visualized using West Femto maximum sensitivity chemiluminescent substrate (Thermo Fisher Scientific).
Lentiviral infection and stable cell generation
To produce lentiviruses encoding RFP, HEK-293 cells were transfected with the lentiviral vector plasmid DNA using Lenti-X Packaging Single Shots (Takara). pCSII-EF-miRFP670v1-hGem(1/110) was a gift from Vladislav Verkhusha (Addgene plasmid # 80006; http://n2t.net/addgene:80006; RRID:Addgene_80006). Supernatants containing lentiviral pseudoparticles were harvested 24 and 48 h post-transfection. harvested lentiviral particles were immediately stored at −80 °C. To establish stable cell lines, 70% confluent cells at one day post-seeding were infected with lentivirus in the presence of 8 µg/mL polybrene (Thermo Fisher Scientific). Two days after transduction, 1 µg/mL puromycin (Thermo Fisher Scientific) was added to the medium to select for stably transduced cells. The samples were visualized daily to ensure that the untransduced cells in the wells were not viable. Once the polyclonal populations had sufficiently expanded, cell stocks were prepared and harvested for protein expression assays.
Cell invasion assay
For cell invasion assays, 8-well chamber slides (Nunc Lab-Tek) were coated with 50 µg/mL poly-L-lysine (Thermo Fisher Scientific) for 30 min and washed with PBS. The slides were fixed with 0.5% glutaraldehyde (Thermo Fisher Scientific) for 20 min and washed. Gelatin was conjugated with fluorescein by mixing 200 µL fluorescein-gelatin (1 mg/mL; ThemoFisher Scientific) with 800 μL unlabeled gelatin (4 mg/mL), followed by incubation for 5 min at 60 °C. The gelatine mix was allowed to cool at RT for 5 min and then applied, and the slides were incubated for 15 min. Slides were washed with PBS and disinfected with 70% ethanol for 30 min. Following three PBS washes, the residual reactive groups were quenched with growth medium by incubating at room temperature for 30 min in the dark. Cells were seeded in a fresh growth medium and incubated undisturbed on a horizontal surface at RT for 30 min to encourage cell distribution. Chamber slides were incubated with 5% CO2 at 37 °C. To detect cell invasion, cells were fixed with 4% formaldehyde and stained with DAPI (Thermo Fisher Scientific), and imaging was performed using a x4/0.2 NA Plan Apo objective (Nikon). Cell invasion was determined by quantifying the sites of the degraded matrix, which were visible as dark areas in the bright-green fluorescent gelatin matrix. The area of gelatin digestion and the number of cells were calculated using the ImageJ software.
Nanoindenter assay for measuring cortical stiffness
Stiffness measurements were performed on live cells in 5% CO2 at 37 °C, maintained in a stage-top chamber (Oko-lab) using a nanoindenter (Optics11 Chiaro) attached to the confocal microscope. Nanoindentation was performed at the cell surface of single cells with an indentation probe with a spring constant (0.24 N/m) and tip diameter (10 μm). The Hertzian contact model was used to fit the data to extract Young’s modulus in the elastic regime.
Surface biotinylation and pull-down assay
ND cells that were 40%–70% confluent and OC cells were detached and washed twice with PBS. The cells were resuspended at a concentration of 25 × 106 cells/mL in PBS containing 2 mM sulfo-NHS-biotin (Invitrogen). The reaction mixture was then incubated at RT for 60 min. Cells were washed three times with 1 M Tris pH 8.0 to quench and remove the excess biotin reagent. Cell pellets were lysed in 1.0 mL of cold lysis buffer (50 mM HEPES pH 7.2, 150 mM NaCl, 1.0% Triton X-100, 1.0% CHAPS, 100x protease and phosphatase inhibitors). Lysates were incubated on ice for 30 min, transferred to tubes, and centrifuged (10,000 × g for 5 min) to remove insoluble material. Supernatants were collected, and protein concentrations were measured using the bicinchoninic acid assay (Pierce). We washed 50 µL resin (ThermoFisher Scientific) with immobilized streptavidin twice with binding buffer (0.1 M phosphate, 0.15 M NaCl pH 7.2) and centrifuged at 5000 x g for 1 min. Biotin-labelled cell lysate (1 mg) was added to the resin and incubated with rotation for 1 h at RT. The resin was washed by resuspending in binding buffer, centrifuging to pellet the resin, and removing the supernatant by aspiration. The wash was repeated four times. Samples were boiled in SDS-PAGE sample buffer with DTT and separated by electrophoresis.
Mass spectrometry
Peptide mixtures from each sample were analyzed by LC-MS/MS using a nano-LC system (Easy nLC1000) connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). The platform was configured with a nano-electrospray ion source (Easy-Spray, ThermoFisher Scientific), an Acclaim PepMap 100 C18 nanoViper trap column (3 μm particle size, 75 μm ID × 20 mm length), and an EASY-Spray C18 analytical column (2 μm particle size, 75 μm ID × 500 mm length). Peptides were eluted at a flow rate of 300 nL/min using linear gradients of 5–25% acetonitrile (in aqueous phase and 0.1% formic acid) for 40 min, followed by 45% for 10 min, and static flow at 90% for 10 min. Mass spectrometry data were collected in a data-dependent manner, switching between one full-scan MS mode (m/z:380–1400; resolution: 60 K; AGC:3e6; maximum ion time:20 ms), and 10 MS/MS scans (resolution: 15 K; AGC:1e5; maximum ion time:120 ms; nCE:27) of the top 10 target ions. Ions were sequenced once and dynamically excluded from the list for 20 s. The datasets were searched using MaxQuant at default parameters against the UniProt Human Proteome database.
Line analysis
IF images of ion channels (TRPV4, PIEZO1, or KCNN4), DiIC18(3), and DAPI were opened in ImageJ. These images were concatenated so that a line crossing a cell in all three images could be used to measure approximate percentage protein localization in the plasma membrane, cytosol, or nucleus. Background was subtracted from all images. DiIC18(3) signal was used as a guide for plasma membrane location (between 50% of the peak intensity) and DAPI signal (between 50% of the peak intensity) for nuclear locations. The cytosol location was defined as the region between the 50% peak intensity points of the DiIC18(3) and DAPI signals. The ion channel signal in each window along the line was then averaged. The percentage of protein localization in the plasma membrane, cytosol, or nucleus for a cell was calculated by dividing the average intensity of each location by the sum of intensities in all three locations.
Pipeline for analyses of patient histologic samples
Specimen collection: A retrospective clinical study was designed to collect patient tissue blocks. A pathologist chose 39 patient breast formalin-fixed paraffin-embedded (FFPE) tissue specimens of a combination of the following pathologies (benign, ADH, IDC, low-, intermediate-, or high-grade DCIS, and IDC), along with normal tissue for each patient. Specimens associated with a past cancer diagnosis and drug treatment history were excluded.
Sample preparation for histopathologic evaluation: For each FFPE block, two serial dissections were carried out. Tissue sections on unlabeled slides were stained with hematoxylin and eosin (H&E) and subjected to immunohistochemical (IHC) staining using TRPV4 antibodies (Abcam 39260; 1:100 dilution). Whole-slide images were captured using the Olympus VS200 whole-slide imaging system. To validate the binding specificity of the antibody, both negative and positive control samples were prepared.
Two sequential annotations: A breast surgical pathologist annotated the pathology stages within specified regions of interest (ROIs) on wholeslide H&E images, based on cell morphological features. Two pathologists independently evaluated the same ROIs in the corresponding IHC images. They annotated the protein distribution using a three-tier classification:
Case 1: Absence of TRPV4.
Case 2: Intracellular TRPV4 localization.
Case 3: Presence of TRPV4 in the plasma membrane, with or without intracellular TRPV4.
The independent annotations concerning protein localization by the two pathologists were then subjected to statistical tests for selectivity and specificity. Any IHC ROIs with classification disagreements between the pathologists were designated as equivocal cases.
Calcium reporter assay
This assay was completed using the Fluo-4 Direct™ Calcium Assay Kit (Thermo Fisher F10471), including the assay buffer (Thermo Fisher), 2X Fluo-4 calcium assay reagent (Thermo Fisher), and preweighed, water-soluble probenecid (Thermo Fisher P36400; 2.5 mM). The 2X Fluo-4 calcium assay reagent was first prepared by adding 10 mL of the Fluo-4 calcium assay buffer and 200 μL of 250 mM probenecid to the desiccated calcium assay reagent at room temperature (21 °C). This mixture was then vortexed and allowed to sit for 15 minutes. While waiting for the 2X Fluo-4 calcium assay reagent to finish being prepared, the media from the well of a LabTek II Chambered Coverglass with Cover #1.5 Borosilicate Sterile 8-well plate (Thermo Fisher 155409) was removed and replaced with 200 μL of complete media. Once the 2X Fluo-4 calcium assay reagent was fully prepared, 200 μL of the reagent was added to the well with 200 μL of complete media. The 8-well plate was then placed at 37 °C and 5% CO₂ for 30 minutes. Following 30 minutes at 37 °C and 5% CO₂, the 8-well plate was removed from the incubator and placed at room temperature for 30 minutes. After incubation in the reagent for 30 minutes at room temperature, the 8-well plate was imaged using the confocal microscope with a 488 nm laser for 30 minutes with 30-second intervals between images and an exposure time of 200 ms. If the sample was treated, the sample was imaged for 35 minutes with 30-second intervals, with the treatment being added after 5 minutes had elapsed. Added treatments all had a final volume of 200 μL and were composed of: 1 nM GSK 2 in 1:1 media:2X Fluo-4 calcium assay reagent, 0.2 nM GSK 2 in 1:1 media:2X Fluo-4 calcium assay reagent, 0.2 pM GSK 1 in 1:1 media:2X Fluo-4 calcium assay reagent, 1:1:1 water:media:2X Fluo-4 calcium assay reagent, and 0.3 Osm/L PEG 300 in 1:1 media:2X Fluo-4 calcium assay reagent.
Cell motility assay
Cells were first seeded in MatTek 35 mm dishes with No. 1.5 coverslip, 14 mm glass diameter, and Poly-D-Lysine coated glass bottoms (MatTek P35GC-1.5-14-C). Once the cells adhered, they were stained with 10 μg/mL WGA-488 (Thermo Fisher W11261) for 10 minutes at room temperature. Following the staining, the cells were washed with complete media twice and then incubated at 37 °C and 5% CO₂ for 1 hour. During this one-hour incubation, the cells received the following treatments: 0.2 pM GSK 101 in complete media for 1 hour, 0.05 pM GSK 101 in complete media for 1 hour, 1 nM GSK 219 in complete media for 1 hour, 0.2 nM GSK 219 in complete media for 1 hour, 74.4 mOsm/L PEG 300 in complete media for 15 minutes, and 1:1 hypoosmotic solution in complete media for 15 minutes. After the incubation, the sample was placed in an OkoLab chamber at 37 °C and 5% CO₂ for imaging. Imaging was completed using a confocal microscope with a 488 nm laser for 3 hours with 1-minute intervals.
Data availability
Data available on reasonable request from the corresponding author (I.C.).
Acknowledgements
I. Chung is supported by the Elsa Pardee Foundation Award, Clinical and Translational Science Institute at Children’s National Fund, George Washington Cross-Disciplinary Research Fund, George Washington Cancer Center, and Katzen Research Cancer Research Pilot Award. We thank M. Sliwkowski, M. Sagola, and Genentech, Inc. for kindly arranging a transfer for an optical microscope and previous scientific discussions, I. Teng for initially assisting with invasion assays, P.F.G. Rodriguez. for helping with setting up the nanoindenter, P. Aswini for assisting with MS data analysis, S. Simmens for statistical consultation, N. Suh for discussion of MCF10A cell derivatives, K. Schill in DigitalScope for assisting with IHC image data management, H. Yoo for summarizing in vivo data, and A. Yohannes and H. Yoo, J. John, and S. Rachidi for managing cells.
Competing Interest
The authors declare no competing non-financial interests but the following competing financial interests: The USPTO has issued a notice of allowance for a patent related to the findings described in this manuscript. The patent application is pending final issuance.
Supplementary information
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